Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0291.Seq
(548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20350.1 68418.m02421 zinc finger (DHHC type) family protein ... 29 1.5
At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive eff... 29 2.0
At3g59110.1 68416.m06590 protein kinase family protein contains ... 28 3.6
At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DN... 28 4.7
At5g47455.3 68418.m05859 expressed protein 27 6.2
At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive eff... 27 6.2
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 27 6.2
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 27 6.2
At2g37260.1 68415.m04571 WRKY family transcription factor (TTG2)... 27 6.2
At5g47455.6 68418.m05857 expressed protein 27 8.3
At5g47455.5 68418.m05855 expressed protein 27 8.3
At5g47455.4 68418.m05860 expressed protein 27 8.3
At5g47455.2 68418.m05858 expressed protein 27 8.3
At5g47455.1 68418.m05856 expressed protein 27 8.3
At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive eff... 27 8.3
At2g21840.1 68415.m02595 CHP-rich zinc finger protein, putative 27 8.3
>At5g20350.1 68418.m02421 zinc finger (DHHC type) family protein /
ankyrin repeat family protein similar to patsas protein
[Drosophila melanogaster] GI:6002770; contains Pfam
profiles PF00023: Ankyrin repeat, PF01529: DHHC zinc
finger domain
Length = 592
Score = 29.5 bits (63), Expect = 1.5
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -1
Query: 365 TGPGLLAWLGLQRSSKAIFVFH-LSRSEPPATRL 267
TG G LAWLG ++ +F+F+ SR +P R+
Sbjct: 279 TGIGALAWLGFILATAGLFLFYRCSRKDPGYIRM 312
>At5g52090.1 68418.m06466 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 676
Score = 29.1 bits (62), Expect = 2.0
Identities = 12/44 (27%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = -3
Query: 318 GHFRVPPVTQRATRDASLSAVR-CLFIVRTESTMRVPGSVWRAI 190
G+ +V + R + +L+ R CL+++ E+T+ + GS+W +
Sbjct: 583 GNGKVGFLNNRQRANVALTRARHCLWVIGNETTLALSGSIWATL 626
>At3g59110.1 68416.m06590 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 512
Score = 28.3 bits (60), Expect = 3.6
Identities = 15/35 (42%), Positives = 20/35 (57%)
Frame = -3
Query: 351 VGLARAAAVLEGHFRVPPVTQRATRDASLSAVRCL 247
VG RA V++ PP T RA + A L A+RC+
Sbjct: 411 VGTRRAEEVVDSRIEPPPAT-RALKRALLVALRCV 444
>At3g23890.1 68416.m03002 DNA topoisomerase, ATP-hydrolyzing / DNA
topoisomerase II / DNA gyrase (TOP2) identical to
SP|P30182 DNA topoisomerase II (EC 5.99.1.3)
{Arabidopsis thaliana}
Length = 1473
Score = 27.9 bits (59), Expect = 4.7
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -3
Query: 546 WYSXSFWTXSRXGSGKSGCTFTIV-MFEEPRSTSI 445
WY T + S + GCT+TI ++EE T+I
Sbjct: 880 WYRGFKGTIEKTASKEGGCTYTITGLYEEVDETTI 914
>At5g47455.3 68418.m05859 expressed protein
Length = 106
Score = 27.5 bits (58), Expect = 6.2
Identities = 19/61 (31%), Positives = 28/61 (45%)
Frame = -1
Query: 542 IPSRSGPXPGLEAXSQAAPSQLXCSKSLVRLRFYCGASVLGSAGCMXGSXRTARGLRPVT 363
IPS+ P PS+L C +SL+ L A+ L S C+ + R++R L
Sbjct: 43 IPSKPAASPLPRFSFSRCPSELGCVQSLLPLHSTVAAARLTS--CLSTTSRSSRALTQEM 100
Query: 362 G 360
G
Sbjct: 101 G 101
>At5g37150.1 68418.m04460 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 839
Score = 27.5 bits (58), Expect = 6.2
Identities = 10/37 (27%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -3
Query: 297 VTQRATRDASLSAVR-CLFIVRTESTMRVPGSVWRAI 190
+ R + +L+ R CL+++ E+T+ + GS+W +
Sbjct: 753 LNNRQRANVALTRARHCLWVIGNETTLALSGSIWATL 789
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 27.5 bits (58), Expect = 6.2
Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +1
Query: 232 RPYDEQTPHG*QRRVAGGSLRDRWNTKMAFEDRCSPSQANSPGPVT--GRSPRAVRXDPX 405
+P+D P A +LR +T++ + R +P SP P + G SP+A+ P
Sbjct: 160 KPFDRSKPSSASAN-APPTLRPA-STRVPSQ-RITPHSVPSPRPSSPRGASPQAISSKPP 216
Query: 406 IQPAEPSTL 432
AEP TL
Sbjct: 217 SPRAEPPTL 225
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 27.5 bits (58), Expect = 6.2
Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +1
Query: 232 RPYDEQTPHG*QRRVAGGSLRDRWNTKMAFEDRCSPSQANSPGPVT--GRSPRAVRXDPX 405
+P+D P A +LR +T++ + R +P SP P + G SP+A+ P
Sbjct: 159 KPFDRSKPSSASAN-APPTLRPA-STRVPSQ-RITPHSVPSPRPSSPRGASPQAISSKPP 215
Query: 406 IQPAEPSTL 432
AEP TL
Sbjct: 216 SPRAEPPTL 224
>At2g37260.1 68415.m04571 WRKY family transcription factor (TTG2)
contains Pfam profile: PF03106 WRKY DNA -binding domain
Length = 349
Score = 27.5 bits (58), Expect = 6.2
Identities = 13/41 (31%), Positives = 20/41 (48%), Gaps = 2/41 (4%)
Frame = -2
Query: 337 GCSGPRRPFSCST--CHAASHPRRVSVSREVFVHRKDGEHN 221
G + PR + C++ C A H R S F+ +G+HN
Sbjct: 284 GNAYPRSYYRCTSANCRARKHVERASDDPRAFITTYEGKHN 324
>At5g47455.6 68418.m05857 expressed protein
Length = 100
Score = 27.1 bits (57), Expect = 8.3
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -1
Query: 542 IPSRSGPXPGLEAXSQAAPSQLXCSKSLVRLRFYCGASVLGSAGCMXGSXRTARGL 375
IPS+ P PS+L C +SL+ L A+ L S C+ + R++R L
Sbjct: 43 IPSKPAASPLPRFSFSRCPSELGCVQSLLPLHSTVAAARLTS--CLSTTSRSSRAL 96
>At5g47455.5 68418.m05855 expressed protein
Length = 108
Score = 27.1 bits (57), Expect = 8.3
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -1
Query: 542 IPSRSGPXPGLEAXSQAAPSQLXCSKSLVRLRFYCGASVLGSAGCMXGSXRTARGL 375
IPS+ P PS+L C +SL+ L A+ L S C+ + R++R L
Sbjct: 43 IPSKPAASPLPRFSFSRCPSELGCVQSLLPLHSTVAAARLTS--CLSTTSRSSRAL 96
>At5g47455.4 68418.m05860 expressed protein
Length = 116
Score = 27.1 bits (57), Expect = 8.3
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -1
Query: 542 IPSRSGPXPGLEAXSQAAPSQLXCSKSLVRLRFYCGASVLGSAGCMXGSXRTARGL 375
IPS+ P PS+L C +SL+ L A+ L S C+ + R++R L
Sbjct: 43 IPSKPAASPLPRFSFSRCPSELGCVQSLLPLHSTVAAARLTS--CLSTTSRSSRAL 96
>At5g47455.2 68418.m05858 expressed protein
Length = 104
Score = 27.1 bits (57), Expect = 8.3
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -1
Query: 542 IPSRSGPXPGLEAXSQAAPSQLXCSKSLVRLRFYCGASVLGSAGCMXGSXRTARGL 375
IPS+ P PS+L C +SL+ L A+ L S C+ + R++R L
Sbjct: 43 IPSKPAASPLPRFSFSRCPSELGCVQSLLPLHSTVAAARLTS--CLSTTSRSSRAL 96
>At5g47455.1 68418.m05856 expressed protein
Length = 100
Score = 27.1 bits (57), Expect = 8.3
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -1
Query: 542 IPSRSGPXPGLEAXSQAAPSQLXCSKSLVRLRFYCGASVLGSAGCMXGSXRTARGL 375
IPS+ P PS+L C +SL+ L A+ L S C+ + R++R L
Sbjct: 43 IPSKPAASPLPRFSFSRCPSELGCVQSLLPLHSTVAAARLTS--CLSTTSRSSRAL 96
>At5g37030.1 68418.m04441 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 638
Score = 27.1 bits (57), Expect = 8.3
Identities = 11/34 (32%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -3
Query: 297 VTQRATRDASLSAVR-CLFIVRTESTMRVPGSVW 199
++ R + +L+ R CL+++ EST+ GS+W
Sbjct: 574 LSNRQRANVALTRARHCLWVIGNESTLARSGSIW 607
>At2g21840.1 68415.m02595 CHP-rich zinc finger protein, putative
Length = 746
Score = 27.1 bits (57), Expect = 8.3
Identities = 10/25 (40%), Positives = 13/25 (52%)
Frame = -2
Query: 319 RPFSCSTCHAASHPRRVSVSREVFV 245
+ FSC TC HP + V R + V
Sbjct: 548 KAFSCVTCEDIYHPMCIQVGRRILV 572
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,060,430
Number of Sequences: 28952
Number of extensions: 216890
Number of successful extensions: 690
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 674
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 690
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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