Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0287.Seq
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 112 4e-26
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 104 1e-23
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 58 6e-10
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 57 1e-09
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 1.8
SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 26 3.2
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 26 4.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 4.2
SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 25 5.6
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 5.6
SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pomb... 25 5.6
SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 25 7.3
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 25 7.3
SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac... 25 9.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 112 bits (269), Expect = 4e-26
Identities = 57/137 (41%), Positives = 75/137 (54%)
Frame = +2
Query: 53 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 232
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 233 HVPRAVFAIXXXXXXMRSALAHTDSCFIQNNLLLVRKMRXXXXXXXXXXLEXKS*IWF*D 412
+VPR+++ + F L+ ++ + + D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 413 RIRKLADQCXGLXGXLI 463
+IR++AD C GL G L+
Sbjct: 121 KIRRIADNCSGLQGFLV 137
Score = 93.9 bits (223), Expect = 1e-20
Identities = 40/50 (80%), Positives = 45/50 (90%)
Frame = +1
Query: 256 DLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGXEIVDLV 405
DLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+G E+VD V
Sbjct: 69 DLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEV 118
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 104 bits (249), Expect = 1e-23
Identities = 60/142 (42%), Positives = 76/142 (53%), Gaps = 5/142 (3%)
Frame = +2
Query: 53 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 217
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 218 TGAGKHVPRAVFAIXXXXXXMRSALAHTDSCFIQNNLLLVRKMRXXXXXXXXXXLEXKS* 397
TG GK VPR+++ + F ++ ++ + +
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 398 IWF*DRIRKLADQCXGLXGXLI 463
+RIR++AD C GL G L+
Sbjct: 120 DSVLERIRRMADNCSGLQGFLV 141
Score = 92.7 bits (220), Expect = 3e-20
Identities = 37/51 (72%), Positives = 46/51 (90%)
Frame = +1
Query: 256 DLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGXEIVDLVL 408
DLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+G E++D VL
Sbjct: 73 DLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVL 123
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 58.4 bits (135), Expect = 6e-10
Identities = 33/96 (34%), Positives = 53/96 (55%), Gaps = 1/96 (1%)
Frame = +2
Query: 56 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 235
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 236 VPRAVFAIXXXXXXMRSALAHT-DSCFIQNNLLLVR 340
+PRA+ I + + L+ T S + N+L+ +
Sbjct: 61 IPRAIL-IDLEPRVVNNILSDTYGSLYNPENILITK 95
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 57.2 bits (132), Expect = 1e-09
Identities = 25/53 (47%), Positives = 35/53 (66%)
Frame = +1
Query: 250 LRDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGXEIVDLVL 408
L DLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VL
Sbjct: 65 LVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVL 117
Score = 49.6 bits (113), Expect = 3e-07
Identities = 25/66 (37%), Positives = 33/66 (50%)
Frame = +2
Query: 53 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 232
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 233 HVPRAV 250
+VPRAV
Sbjct: 59 YVPRAV 64
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 27.1 bits (57), Expect = 1.8
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = +3
Query: 132 STASSLMARCPQTRPSGVETILSTLSSARPELASTXPVLSS 254
ST SSL + ++PS T ST SSA P S+ + SS
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSS 213
Score = 24.6 bits (51), Expect = 9.7
Identities = 21/66 (31%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
Frame = +3
Query: 60 SASLYTLAKPESRSVMPAGSFTAWSTASSL-MARCPQTRPSGVETILSTLSSARPELAST 236
S+S + + S S P+ S ++ T+SSL + P T S T S SS+ AS+
Sbjct: 206 SSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASS 265
Query: 237 XPVLSS 254
SS
Sbjct: 266 SSSSSS 271
>SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1496
Score = 26.2 bits (55), Expect = 3.2
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +1
Query: 328 ITGKEDAANNYARGHYTIGXEIVDLVLGQN 417
IT + NN GH T+G + V L LG +
Sbjct: 883 ITDFSRSVNNQRNGHLTVGSDAVCLSLGDS 912
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 25.8 bits (54), Expect = 4.2
Identities = 11/38 (28%), Positives = 17/38 (44%)
Frame = -3
Query: 288 ADLINNSRFKIAKTARGTCLPAPVSLKKVLKESSPPPM 175
AD+IN SR+ I + + T + L P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 4.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 151 WPDAHRQDHRGWRRFFQHFLQR 216
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC11E3.11c |||guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 942
Score = 25.4 bits (53), Expect = 5.6
Identities = 30/99 (30%), Positives = 49/99 (49%), Gaps = 6/99 (6%)
Frame = +3
Query: 39 KLKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARC----PQTRPSGVETILSTL 206
K KSK ++ + P S+SVM S T+ T +S +R R G+ T +S++
Sbjct: 85 KDKSKASMSTNDLPSHPRSQSVMGFSSSTSQLTGTSNSSRTRLNKDMRRDFGM-TSMSSI 143
Query: 207 SSARPELASTXPVLSSRS*TYC--C**GPHWHIQTVVSS 317
+S+ P S PV S S ++ GP + +T++SS
Sbjct: 144 TSSTP-TPSQLPVRPSTSLSFFDDIPLGPSFSAETILSS 181
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 5.6
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 42 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 164
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 983
Score = 25.4 bits (53), Expect = 5.6
Identities = 24/83 (28%), Positives = 36/83 (43%), Gaps = 6/83 (7%)
Frame = +3
Query: 24 FKHRKKLKSKCVSASLYTLAK----PESRS--VMPAGSFTAWSTASSLMARCPQTRPSGV 185
F + K KS S LYT+ + PES + A + WST + + P+T +
Sbjct: 648 FVNAIKAKSFEFSTFLYTVIEYCVNPESPEHVIFEADAMELWSTFLMYIQKLPETFTLLI 707
Query: 186 ETILSTLSSARPELASTXPVLSS 254
+L LS A L ++SS
Sbjct: 708 PHLLYHLSQATSTLPFVLMIVSS 730
>SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 404
Score = 25.0 bits (52), Expect = 7.3
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = -2
Query: 418 DSVLKPNLRFPFQWCSDH 365
DS+L+ L+ WCS+H
Sbjct: 163 DSILQQELKEVLSWCSEH 180
>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 400
Score = 25.0 bits (52), Expect = 7.3
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -2
Query: 106 ITDLDSGLANVYRDALTHFDLSF 38
+T +D GLA YRD TH + +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168
>SPBC3H7.15 |hhp1||serine/threonine protein kinase
Hhp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 365
Score = 24.6 bits (51), Expect = 9.7
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
Frame = -2
Query: 160 HLAIRLDAVLQAV-KLPAGITDLDSGLANVYRDALTHFDLSF 38
H I+ D L + K + +D GLA YRD TH + +
Sbjct: 128 HRDIKPDNFLMGIGKRGNQVNIIDFGLAKKYRDHKTHLHIPY 169
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,988,867
Number of Sequences: 5004
Number of extensions: 38948
Number of successful extensions: 126
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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