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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0271.Seq
         (499 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC336.10c |tif512||translation initiation factor|Schizosacchar...   118   6e-28
SPAC26H5.10c |tif51||translation initiation factor eIF5A|Schizos...   118   6e-28
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic...    27   2.1  
SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|ch...    27   2.1  
SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa...    25   4.8  
SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces po...    25   6.3  
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos...    25   8.4  
SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar...    25   8.4  

>SPBC336.10c |tif512||translation initiation
           factor|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 157

 Score =  118 bits (283), Expect = 6e-28
 Identities = 52/76 (68%), Positives = 62/76 (81%)
 Frame = +1

Query: 49  MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 228
           M + E   FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+
Sbjct: 1   MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60

Query: 229 VGIDIFNGKSMKISVP 276
           V +DIFNG+  +   P
Sbjct: 61  VALDIFNGRKYEDMSP 76



 Score =   99 bits (238), Expect = 2e-22
 Identities = 42/81 (51%), Positives = 62/81 (76%)
 Frame = +3

Query: 255 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGXQLRXD 434
           KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM  +G  ++D+++P+G+LG ++   
Sbjct: 70  KYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGNEIEEG 128

Query: 435 FDSGKELLCTVLKSCGEECVI 497
           F+ GK+L+ TV+ + GEE  +
Sbjct: 129 FEEGKDLIITVVSAMGEEIAL 149


>SPAC26H5.10c |tif51||translation initiation factor
           eIF5A|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score =  118 bits (283), Expect = 6e-28
 Identities = 52/76 (68%), Positives = 62/76 (81%)
 Frame = +1

Query: 49  MGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHL 228
           M + E   FE G++GAS TFPMQCSALRKNG V++KGRPCKIV+MSTSKTGKHGHAKVH+
Sbjct: 1   MAEEEHVDFEGGEAGASLTFPMQCSALRKNGHVVIKGRPCKIVDMSTSKTGKHGHAKVHI 60

Query: 229 VGIDIFNGKSMKISVP 276
           V +DIFNG+  +   P
Sbjct: 61  VALDIFNGRKYEDMSP 76



 Score =  100 bits (239), Expect = 1e-22
 Identities = 42/81 (51%), Positives = 62/81 (76%)
 Frame = +3

Query: 255 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGXQLRXD 434
           KYED+ PSTHNMDVP VKR++YQL +I DDGYL LM  +G  ++D+++P+G+LG ++   
Sbjct: 70  KYEDMSPSTHNMDVPVVKRDEYQLVNI-DDGYLNLMTTDGTTKDDVRLPEGELGNEIEEG 128

Query: 435 FDSGKELLCTVLKSCGEECVI 497
           FD G++L+ TV+ + GEE  +
Sbjct: 129 FDEGRDLIITVVSAMGEETAL 149


>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
            subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1729

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 9/23 (39%), Positives = 15/23 (65%)
 Frame = +3

Query: 285  NMDVPHVKREDYQLTDISDDGYL 353
            NM +P+++ E  Q+ D + D YL
Sbjct: 968  NMSIPYIRGEYVQMIDANQDNYL 990


>SPCC825.04c |||N-acetyltransferase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 204

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +3

Query: 141 FRYAEGSSMQDC*NVHIQNRKARPR*SSLGWD 236
           + + E   +Q C N+  +N +A  R SS GWD
Sbjct: 41  YHHLEKGLLQQCFNLVKKNMEALYRQSSFGWD 72


>SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase
           Ubp7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 875

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 10/17 (58%), Positives = 14/17 (82%)
 Frame = +3

Query: 303 VKREDYQLTDISDDGYL 353
           +KREDY+LT  ++ GYL
Sbjct: 91  IKREDYELTLCANCGYL 107


>SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 594

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = +3

Query: 255 KYEDICPSTHNMDVPHVKRED 317
           K +D CPST   D+ H+ R D
Sbjct: 186 KLQDRCPSTSLKDIDHLFRVD 206


>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
           Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 449

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 12/49 (24%), Positives = 22/49 (44%), Gaps = 1/49 (2%)
 Frame = +1

Query: 109 PMQCSALRKNGFVMLKGRPCKIVEMSTSKTGK-HGHAKVHLVGIDIFNG 252
           P +CS  ++     ++   C  V       G+ H     H++G+D+ NG
Sbjct: 35  PRRCSTCKRINKRSIRCLSCHSVGCLWGHHGEEHAMEHTHMIGVDVKNG 83


>SPAC57A7.04c |pabp||mRNA export shuttling protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 653

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 12/40 (30%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
 Frame = +1

Query: 124 ALRKNGFVMLKGRPCKIV-EMSTSKTGKHGHAKVHLVGID 240
           AL +  + ++KGRPC+I+         K G   V +  +D
Sbjct: 137 ALDELNYTLIKGRPCRIMWSQRDPSLRKMGTGNVFIKNLD 176


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,107,721
Number of Sequences: 5004
Number of extensions: 43090
Number of successful extensions: 152
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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