Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0271.Seq
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 105 2e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 101 2e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 97 8e-21
At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 30 1.00
At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 2.3
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 2.3
At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 4.0
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 4.0
At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 5.3
At3g51290.1 68416.m05614 proline-rich family protein 27 7.0
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 7.0
At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 27 7.0
At1g56500.1 68414.m06497 haloacid dehalogenase-like hydrolase fa... 27 7.0
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 27 9.3
At4g12460.1 68417.m01971 oxysterol-binding family protein simila... 27 9.3
At4g11430.1 68417.m01841 hydroxyproline-rich glycoprotein family... 27 9.3
At3g09770.2 68416.m01158 zinc finger (C3HC4-type RING finger) fa... 27 9.3
At3g09770.1 68416.m01157 zinc finger (C3HC4-type RING finger) fa... 27 9.3
At1g34355.1 68414.m04265 forkhead-associated domain-containing p... 27 9.3
At1g12380.1 68414.m01431 expressed protein 27 9.3
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 105 bits (252), Expect = 2e-23
Identities = 43/72 (59%), Positives = 56/72 (77%)
Frame = +1
Query: 61 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 240
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 241 IFNGKSMKISVP 276
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
Score = 99 bits (238), Expect = 8e-22
Identities = 44/79 (55%), Positives = 60/79 (75%), Gaps = 1/79 (1%)
Frame = +3
Query: 255 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGXQLRX 431
K EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q++
Sbjct: 69 KLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIKS 128
Query: 432 DFDSGKELLCTVLKSCGEE 488
FD GK+L+ +V+ + GEE
Sbjct: 129 GFDDGKDLVVSVMSAMGEE 147
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 101 bits (243), Expect = 2e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +1
Query: 61 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 240
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 241 IFNGKSMKISVP 276
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
Score = 94.7 bits (225), Expect = 3e-20
Identities = 42/79 (53%), Positives = 59/79 (74%), Gaps = 1/79 (1%)
Frame = +3
Query: 255 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGXQLRX 431
K EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L QL+
Sbjct: 69 KLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLKN 128
Query: 432 DFDSGKELLCTVLKSCGEE 488
F+ GK+++ +V+ + GEE
Sbjct: 129 GFEEGKDIVVSVMSAMGEE 147
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 96.7 bits (230), Expect = 8e-21
Identities = 40/72 (55%), Positives = 52/72 (72%)
Frame = +1
Query: 61 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 240
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 241 IFNGKSMKISVP 276
IF K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
Score = 95.9 bits (228), Expect = 1e-20
Identities = 42/79 (53%), Positives = 59/79 (74%), Gaps = 1/79 (1%)
Frame = +3
Query: 255 KYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGXQLRX 431
K EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R
Sbjct: 69 KLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMRL 128
Query: 432 DFDSGKELLCTVLKSCGEE 488
FD GK+++ +V+ S GEE
Sbjct: 129 GFDEGKDIVVSVMSSMGEE 147
>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 322
Score = 29.9 bits (64), Expect = 1.00
Identities = 20/69 (28%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = -3
Query: 470 QHGAQQFLAAVEVXTQLXAEVTIRDFEVLTQVTIV-SHQGQVAIIRDISQLVVFALHVGY 294
+HGA + AA++V L +RD E +V V S + + + D++ ++V G
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232
Query: 293 VHVVCGGTD 267
+ CGG D
Sbjct: 233 RELSCGGDD 241
>At5g41460.1 68418.m05035 fringe-related protein strong similarity
to unknown protein (pir||T13026) similarity to predicted
proteins + similar to hypothetical protein GB:AAC23643
[Arabidopsis thaliana] + weak similarity to Fringe
[Schistocerca gregaria](GI:6573138);Fringe encodes an
extracellular protein that regulates Notch signalling.
Length = 524
Score = 28.7 bits (61), Expect = 2.3
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +2
Query: 26 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 112
++ F T Q K++ S P +P P PP P
Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 2.3
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -3
Query: 161 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 54
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At3g53330.1 68416.m05884 plastocyanin-like domain-containing
protein similar to mavicyanin SP:P80728 from [Cucurbita
pepo]
Length = 310
Score = 27.9 bits (59), Expect = 4.0
Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%)
Frame = +2
Query: 59 SKTHT-SRPETPGPQPPS 109
SKTH SRP TP P PPS
Sbjct: 127 SKTHERSRPITPSPPPPS 144
Score = 26.6 bits (56), Expect = 9.3
Identities = 12/17 (70%), Positives = 12/17 (70%), Gaps = 1/17 (5%)
Frame = +2
Query: 59 SKTHT-SRPETPGPQPP 106
SKTH SRP TP P PP
Sbjct: 144 SKTHEPSRPNTPPPPPP 160
>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
contains INTERPRO domain, IPR001878: Zn-finger CCHC type
Length = 353
Score = 27.9 bits (59), Expect = 4.0
Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%)
Frame = +2
Query: 62 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 187
KT T P PP PCN PC S + + R CP
Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66
>At1g20860.1 68414.m02613 phosphate transporter family protein
similar to phosphate transporter [Catharanthus roseus]
GI:2208908, inorganic phosphate transporter 1 [Solanum
tuberosum] GI:1420871; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 534
Score = 27.5 bits (58), Expect = 5.3
Identities = 17/45 (37%), Positives = 20/45 (44%)
Frame = +2
Query: 38 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 172
K Q V S++H S T P PP P P K S C +H R
Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284
>At3g51290.1 68416.m05614 proline-rich family protein
Length = 602
Score = 27.1 bits (57), Expect = 7.0
Identities = 10/25 (40%), Positives = 13/25 (52%)
Frame = +2
Query: 38 KTQQWVTSKTHTSRPETPGPQPPSP 112
+T W T+ T + P P P PP P
Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.1 bits (57), Expect = 7.0
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
Frame = +3
Query: 333 ISDDGYLTLMADNG--DLREDLKIPDGDLGXQLRX----DFDSGKELLCTV 467
+SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At2g27680.1 68415.m03354 aldo/keto reductase family protein
contains Pfam profile PF00248: oxidoreductase, aldo/keto
reductase family
Length = 384
Score = 27.1 bits (57), Expect = 7.0
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +3
Query: 261 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKI 398
++I S MDV + + D ++DGYL + DL+E+ KI
Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKEEGKI 192
>At1g56500.1 68414.m06497 haloacid dehalogenase-like hydrolase
family protein low similarity to SP|P95649 CbbY protein
{Rhodobacter sphaeroides}; contains InterPro accession
IPR005834: Haloacid dehalogenase-like hydrolase
Length = 1055
Score = 27.1 bits (57), Expect = 7.0
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = -3
Query: 446 AAVEVXTQLXAEVTIRDFEVLTQVTIVSHQGQVAIIRDI 330
AAV+V T++ EVT+ DF G VA ++++
Sbjct: 95 AAVDVFTEMGVEVTVDDFVPFMGTGEAKFLGGVASVKEV 133
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 26.6 bits (56), Expect = 9.3
Identities = 14/34 (41%), Positives = 15/34 (44%)
Frame = +3
Query: 390 LKIPDGDLGXQLRXDFDSGKELLCTVLKSCGEEC 491
L IPDGD L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At4g12460.1 68417.m01971 oxysterol-binding family protein similar
to SP|P22059 Oxysterol-binding protein 1 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 694
Score = 26.6 bits (56), Expect = 9.3
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +1
Query: 64 DTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCK 171
D +E G SG S + +A +G+ +GR CK
Sbjct: 367 DQAYEYGKSGKSLLRALNVAAFAVSGYASTEGRHCK 402
>At4g11430.1 68417.m01841 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965; related to hydroxyproline-rich
glycoprotein [Phaseolus vulgaris] gi|169349|gb|AAA33765
Length = 219
Score = 26.6 bits (56), Expect = 9.3
Identities = 16/50 (32%), Positives = 19/50 (38%)
Frame = +2
Query: 68 HTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKPESTATL 217
H P P P PP P + P V T + H R P P P T+
Sbjct: 116 HRRSPPPPPPPPPPPPTITPPV-TTTTAGHHHHRRSPPPPPPPPPPPPTI 164
>At3g09770.2 68416.m01158 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 341
Score = 26.6 bits (56), Expect = 9.3
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = +2
Query: 62 KTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKP 199
+ HT+ P P P PPS P + T +V A + P+P P
Sbjct: 17 RNHTAAPPPP-PPPPSSSLPPPPLPTEIQANPIVFAAVTPYPNPNP 61
>At3g09770.1 68416.m01157 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 388
Score = 26.6 bits (56), Expect = 9.3
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = +2
Query: 62 KTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKP 199
+ HT+ P P P PPS P + T +V A + P+P P
Sbjct: 17 RNHTAAPPPP-PPPPSSSLPPPPLPTEIQANPIVFAAVTPYPNPNP 61
>At1g34355.1 68414.m04265 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 1477
Score = 26.6 bits (56), Expect = 9.3
Identities = 9/28 (32%), Positives = 15/28 (53%)
Frame = +1
Query: 34 IQNTTMGDIEDTHFETGDSGASATFPMQ 117
+ NT +GD ED H + G ++ P +
Sbjct: 341 LANTALGDDEDLHLDVTSEGTGSSVPSE 368
>At1g12380.1 68414.m01431 expressed protein
Length = 793
Score = 26.6 bits (56), Expect = 9.3
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +2
Query: 56 TSKTHTSRPETPGPQPPSP 112
T+ HT+ +TP P PP P
Sbjct: 3 TTNAHTATQQTPPPPPPPP 21
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,396,639
Number of Sequences: 28952
Number of extensions: 247727
Number of successful extensions: 819
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 738
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 805
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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