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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0270.Seq
         (498 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce...    86   3e-18
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ...    42   7e-05
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom...    25   4.8  
SPBC215.08c |arg4||carbamoyl-phosphate synthase Arg4|Schizosacch...    25   6.3  
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    25   6.3  
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc...    25   8.4  
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce...    25   8.4  

>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 568

 Score = 85.8 bits (203), Expect = 3e-18
 Identities = 41/72 (56%), Positives = 49/72 (68%)
 Frame = -2

Query: 491 KIKSFKQCRAAWTTAASTESVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYW 312
           +IKSF   R        TESVD LMP VGEIVGGS R  D +E +  YKREGIDP+P YW
Sbjct: 463 EIKSFYMKRVV-DRPELTESVDCLMPNVGEIVGGSMRISDIQELLAAYKREGIDPAPYYW 521

Query: 311 YTDQRKFGSVPH 276
           +T+QRK+G+  H
Sbjct: 522 FTEQRKYGTTEH 533



 Score = 56.0 bits (129), Expect = 3e-09
 Identities = 21/30 (70%), Positives = 24/30 (80%)
 Frame = -1

Query: 261 GFERFLCWLLDRYHIRDVCLYPRFLERCTP 172
           G ERFL WL DRY +R+ CL+PRF ERCTP
Sbjct: 539 GLERFLAWLCDRYTVRECCLFPRFTERCTP 568


>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 441

 Score = 41.5 bits (93), Expect = 7e-05
 Identities = 27/72 (37%), Positives = 36/72 (50%)
 Frame = -2

Query: 491 KIKSFKQCRAAWTTAASTESVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYW 312
           KIK F    +   T A+   VD+L+P VGE+ GGS R  DH +  + Y      P    W
Sbjct: 344 KIKPFYMKSSGPDTVAA---VDLLVPQVGELAGGSLRK-DHLDEYKTY------PPELQW 393

Query: 311 YTDQRKFGSVPH 276
           Y D  K+ + PH
Sbjct: 394 YLDLMKYSNAPH 405


>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1562

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 14/32 (43%), Positives = 16/32 (50%), Gaps = 1/32 (3%)
 Frame = +2

Query: 215 RMWYRSRSQQRKRSNPARSRRE-APSRTCAGP 307
           R W RSRSQ+    +  RS  E  P RT   P
Sbjct: 340 RRWRRSRSQESASRSTIRSTDEREPERTSEDP 371


>SPBC215.08c |arg4||carbamoyl-phosphate synthase
            Arg4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1160

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 9/25 (36%), Positives = 15/25 (60%)
 Frame = -2

Query: 464  AAWTTAASTESVDVLMPGVGEIVGG 390
            A W    ST++  + +PG G ++GG
Sbjct: 1003 AYWAALQSTQNFKIPLPGQGILLGG 1027


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 15/62 (24%), Positives = 30/62 (48%)
 Frame = +2

Query: 107 QYTQRHVIRQNKDDTSSGRGAQGVHRSRKRG*RHTSRMWYRSRSQQRKRSNPARSRREAP 286
           Q   RH + +  + T+S R +  V R +    R+T+ +     S++   + P+ +  + P
Sbjct: 3   QTNSRHSLIETDEPTTSSRVSMLVDRVKLLWKRYTTPLSTEDGSREAHFAVPSENYADTP 62

Query: 287 SR 292
           SR
Sbjct: 63  SR 64


>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 498

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 10/19 (52%), Positives = 13/19 (68%)
 Frame = -2

Query: 458 WTTAASTESVDVLMPGVGE 402
           W TAAS ++ DV  PG+ E
Sbjct: 32  WVTAASGKTFDVENPGLNE 50


>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1067

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
 Frame = -2

Query: 437  ESVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYWYTD--QRKFGSV 282
            ++V   + G+ EI+       D E+ +  Y +EG+   P  WY D  +R F ++
Sbjct: 989  KTVAAKLSGMKEILFPKSNLADWEQ-LPDYVKEGLTGVPVAWYDDVFKRVFSNI 1041


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,582,256
Number of Sequences: 5004
Number of extensions: 26238
Number of successful extensions: 65
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 62
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 64
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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