BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0270.Seq (498 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 86 3e-18 SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 42 7e-05 SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 25 4.8 SPBC215.08c |arg4||carbamoyl-phosphate synthase Arg4|Schizosacch... 25 6.3 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 6.3 SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 25 8.4 SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 25 8.4 >SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces pombe|chr 2|||Manual Length = 568 Score = 85.8 bits (203), Expect = 3e-18 Identities = 41/72 (56%), Positives = 49/72 (68%) Frame = -2 Query: 491 KIKSFKQCRAAWTTAASTESVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYW 312 +IKSF R TESVD LMP VGEIVGGS R D +E + YKREGIDP+P YW Sbjct: 463 EIKSFYMKRVV-DRPELTESVDCLMPNVGEIVGGSMRISDIQELLAAYKREGIDPAPYYW 521 Query: 311 YTDQRKFGSVPH 276 +T+QRK+G+ H Sbjct: 522 FTEQRKYGTTEH 533 Score = 56.0 bits (129), Expect = 3e-09 Identities = 21/30 (70%), Positives = 24/30 (80%) Frame = -1 Query: 261 GFERFLCWLLDRYHIRDVCLYPRFLERCTP 172 G ERFL WL DRY +R+ CL+PRF ERCTP Sbjct: 539 GLERFLAWLCDRYTVRECCLFPRFTERCTP 568 >SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 41.5 bits (93), Expect = 7e-05 Identities = 27/72 (37%), Positives = 36/72 (50%) Frame = -2 Query: 491 KIKSFKQCRAAWTTAASTESVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYW 312 KIK F + T A+ VD+L+P VGE+ GGS R DH + + Y P W Sbjct: 344 KIKPFYMKSSGPDTVAA---VDLLVPQVGELAGGSLRK-DHLDEYKTY------PPELQW 393 Query: 311 YTDQRKFGSVPH 276 Y D K+ + PH Sbjct: 394 YLDLMKYSNAPH 405 >SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1562 Score = 25.4 bits (53), Expect = 4.8 Identities = 14/32 (43%), Positives = 16/32 (50%), Gaps = 1/32 (3%) Frame = +2 Query: 215 RMWYRSRSQQRKRSNPARSRRE-APSRTCAGP 307 R W RSRSQ+ + RS E P RT P Sbjct: 340 RRWRRSRSQESASRSTIRSTDEREPERTSEDP 371 >SPBC215.08c |arg4||carbamoyl-phosphate synthase Arg4|Schizosaccharomyces pombe|chr 2|||Manual Length = 1160 Score = 25.0 bits (52), Expect = 6.3 Identities = 9/25 (36%), Positives = 15/25 (60%) Frame = -2 Query: 464 AAWTTAASTESVDVLMPGVGEIVGG 390 A W ST++ + +PG G ++GG Sbjct: 1003 AYWAALQSTQNFKIPLPGQGILLGG 1027 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 25.0 bits (52), Expect = 6.3 Identities = 15/62 (24%), Positives = 30/62 (48%) Frame = +2 Query: 107 QYTQRHVIRQNKDDTSSGRGAQGVHRSRKRG*RHTSRMWYRSRSQQRKRSNPARSRREAP 286 Q RH + + + T+S R + V R + R+T+ + S++ + P+ + + P Sbjct: 3 QTNSRHSLIETDEPTTSSRVSMLVDRVKLLWKRYTTPLSTEDGSREAHFAVPSENYADTP 62 Query: 287 SR 292 SR Sbjct: 63 SR 64 >SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 498 Score = 24.6 bits (51), Expect = 8.4 Identities = 10/19 (52%), Positives = 13/19 (68%) Frame = -2 Query: 458 WTTAASTESVDVLMPGVGE 402 W TAAS ++ DV PG+ E Sbjct: 32 WVTAASGKTFDVENPGLNE 50 >SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1067 Score = 24.6 bits (51), Expect = 8.4 Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 2/54 (3%) Frame = -2 Query: 437 ESVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYWYTD--QRKFGSV 282 ++V + G+ EI+ D E+ + Y +EG+ P WY D +R F ++ Sbjct: 989 KTVAAKLSGMKEILFPKSNLADWEQ-LPDYVKEGLTGVPVAWYDDVFKRVFSNI 1041 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,582,256 Number of Sequences: 5004 Number of extensions: 26238 Number of successful extensions: 65 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 62 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 64 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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