BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0270.Seq
(498 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g70980.1 68414.m08188 asparaginyl-tRNA synthetase, cytoplasmi... 49 2e-06
At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplas... 48 3e-06
At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplas... 46 1e-05
At3g62330.1 68416.m07002 zinc knuckle (CCHC-type) family protein... 35 0.026
At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 30 1.00
At1g61290.1 68414.m06908 syntaxin, putative (SYP124) similar to ... 28 3.0
At1g11250.1 68414.m01288 syntaxin, putative (SYP125) similar to ... 27 5.3
At5g58610.1 68418.m07345 PHD finger transcription factor, putative 27 7.0
At2g31660.1 68415.m03865 importin beta-2 subunit family protein ... 27 7.0
>At1g70980.1 68414.m08188 asparaginyl-tRNA synthetase, cytoplasmic,
putative / asparagine-tRNA ligase, putative similar to
SYNC1 protein GI:5670315 [SP|Q9SW96] from [Arabidopsis
thaliana]
Length = 571
Score = 49.2 bits (112), Expect = 2e-06
Identities = 20/53 (37%), Positives = 33/53 (62%)
Frame = -2
Query: 434 SVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYWYTDQRKFGSVPH 276
++DVL+P VGE++GGS R ++ + + G+ P WY D R++G+V H
Sbjct: 484 AMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKH 536
>At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplasmic
/ asparagine-tRNA ligase 1 (SYNC1) identical to
SP|Q9SW96
Length = 572
Score = 48.4 bits (110), Expect = 3e-06
Identities = 20/53 (37%), Positives = 33/53 (62%)
Frame = -2
Query: 434 SVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYWYTDQRKFGSVPH 276
++DVL+P VGE++GGS R ++ + + G+ P WY D R++G+V H
Sbjct: 485 AMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKH 537
>At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplast,
mitochondrial / asparagine-tRNA ligase / AsnRS (SYNO)
nearly identical to SP|O48593
Length = 567
Score = 46.0 bits (104), Expect = 1e-05
Identities = 21/54 (38%), Positives = 35/54 (64%), Gaps = 1/54 (1%)
Frame = -2
Query: 434 SVDVLMPGVGEIVGGSXRXWDHEEXMEGYKRE-GIDPSPXYWYTDQRKFGSVPH 276
++D+L+P +GE++GGS R + E +E E ++ +WY D R++GSVPH
Sbjct: 480 AMDMLVPRIGELIGGSQRE-ERLEVLEARLDELKLNKESYWWYLDLRRYGSVPH 532
>At3g62330.1 68416.m07002 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 479
Score = 35.1 bits (77), Expect = 0.026
Identities = 18/61 (29%), Positives = 34/61 (55%), Gaps = 2/61 (3%)
Frame = +2
Query: 116 QRHVIRQNKDDTSSGRGAQGVHRSRKRG*RHTSRMWYRSRSQQRKRSN--PARSRREAPS 289
++ +I KD +G VH+ ++ G +S + +RSRS+ +R++ P+R+R P
Sbjct: 155 EKFIIVSGKDRLILRKGVDAVHKVKEDGEMKSSSVSHRSRSRSPRRTSVGPSRARNSEPQ 214
Query: 290 R 292
R
Sbjct: 215 R 215
>At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative simialr to
aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila
melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus
norvegicus SP|P15178; contains Pfam profile PF00152 tRNA
synthetases class II (D, K and N)
Length = 532
Score = 29.9 bits (64), Expect = 1.00
Identities = 21/56 (37%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Frame = -2
Query: 440 TESVDVLMPGVGEIVGGSXRXWDHEEXMEGYKRE-GIDPSPXYWYTDQRKFGSVPH 276
+ S DV + G EI+ G+ R D E +E RE GID Y D ++G+ PH
Sbjct: 444 SNSFDVFIRGE-EIMSGAQRIHD-PELLEKRARECGIDVKTISTYIDAFRYGAPPH 497
>At1g61290.1 68414.m06908 syntaxin, putative (SYP124) similar to
syntaxin-related protein Nt-syr1 GI:4206787 from
[Nicotiana tabacum]
Length = 303
Score = 28.3 bits (60), Expect = 3.0
Identities = 18/68 (26%), Positives = 36/68 (52%)
Frame = +3
Query: 135 KIKTIRRRVEALRACTALGSEGRDTRPGCGTGPGASRGSAQTQPVAAVRHRAELALVRIP 314
++K I++++EAL A ++R G GPG+S +T V+ + + + +
Sbjct: 90 RVKMIKQKLEALEKANA------NSRNVSGCGPGSSTDRTRTSVVSGLGKKLKDLMDSFQ 143
Query: 315 VVRARVDA 338
+RAR++A
Sbjct: 144 GLRARMNA 151
>At1g11250.1 68414.m01288 syntaxin, putative (SYP125) similar to
syntaxin-related protein At-SYR1 GB:AAD11809 GI:4206789
from [Arabidopsis thaliana]
Length = 298
Score = 27.5 bits (58), Expect = 5.3
Identities = 19/71 (26%), Positives = 38/71 (53%)
Frame = +3
Query: 123 MLSGKIKTIRRRVEALRACTALGSEGRDTRPGCGTGPGASRGSAQTQPVAAVRHRAELAL 302
M+ ++K I++++EAL A R+ PGC GPG+S ++ V+ + + + +
Sbjct: 81 MVLKRVKIIKQKLEALEKANA---NSRNV-PGC--GPGSSTDRTRSSVVSGLGKKLKDLM 134
Query: 303 VRIPVVRARVD 335
+RAR++
Sbjct: 135 DSFQGLRARMN 145
>At5g58610.1 68418.m07345 PHD finger transcription factor, putative
Length = 1065
Score = 27.1 bits (57), Expect = 7.0
Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 2/42 (4%)
Frame = +2
Query: 164 GAQGVHRSRKRG*RHTSR--MWYRSRSQQRKRSNPARSRREA 283
G Q V+RS +RG HTS+ M ++R R ++ RSR+ A
Sbjct: 535 GLQNVNRSMRRG--HTSKKLMDIKNRVTSRGKTRVLRSRKRA 574
>At2g31660.1 68415.m03865 importin beta-2 subunit family protein
similar to D-Importin 7/RanBP7 [Drosophila melanogaster]
GI:7542336; contains Pfam profile PF03810: Importin-beta
N-terminal domain
Length = 1040
Score = 27.1 bits (57), Expect = 7.0
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +2
Query: 218 MWYRSRSQQRKRSNPARSRREAPSRTCAGPYTS 316
+W++ Q+RK PA +RE + C TS
Sbjct: 823 LWFQMLQQKRKSGLPANFKREHDKKVCCLGLTS 855
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,353,252
Number of Sequences: 28952
Number of extensions: 143837
Number of successful extensions: 440
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 413
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 436
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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