BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0270.Seq (498 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g70980.1 68414.m08188 asparaginyl-tRNA synthetase, cytoplasmi... 49 2e-06 At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplas... 48 3e-06 At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplas... 46 1e-05 At3g62330.1 68416.m07002 zinc knuckle (CCHC-type) family protein... 35 0.026 At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 30 1.00 At1g61290.1 68414.m06908 syntaxin, putative (SYP124) similar to ... 28 3.0 At1g11250.1 68414.m01288 syntaxin, putative (SYP125) similar to ... 27 5.3 At5g58610.1 68418.m07345 PHD finger transcription factor, putative 27 7.0 At2g31660.1 68415.m03865 importin beta-2 subunit family protein ... 27 7.0 >At1g70980.1 68414.m08188 asparaginyl-tRNA synthetase, cytoplasmic, putative / asparagine-tRNA ligase, putative similar to SYNC1 protein GI:5670315 [SP|Q9SW96] from [Arabidopsis thaliana] Length = 571 Score = 49.2 bits (112), Expect = 2e-06 Identities = 20/53 (37%), Positives = 33/53 (62%) Frame = -2 Query: 434 SVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYWYTDQRKFGSVPH 276 ++DVL+P VGE++GGS R ++ + + G+ P WY D R++G+V H Sbjct: 484 AMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKH 536 >At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplasmic / asparagine-tRNA ligase 1 (SYNC1) identical to SP|Q9SW96 Length = 572 Score = 48.4 bits (110), Expect = 3e-06 Identities = 20/53 (37%), Positives = 33/53 (62%) Frame = -2 Query: 434 SVDVLMPGVGEIVGGSXRXWDHEEXMEGYKREGIDPSPXYWYTDQRKFGSVPH 276 ++DVL+P VGE++GGS R ++ + + G+ P WY D R++G+V H Sbjct: 485 AMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKH 537 >At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplast, mitochondrial / asparagine-tRNA ligase / AsnRS (SYNO) nearly identical to SP|O48593 Length = 567 Score = 46.0 bits (104), Expect = 1e-05 Identities = 21/54 (38%), Positives = 35/54 (64%), Gaps = 1/54 (1%) Frame = -2 Query: 434 SVDVLMPGVGEIVGGSXRXWDHEEXMEGYKRE-GIDPSPXYWYTDQRKFGSVPH 276 ++D+L+P +GE++GGS R + E +E E ++ +WY D R++GSVPH Sbjct: 480 AMDMLVPRIGELIGGSQRE-ERLEVLEARLDELKLNKESYWWYLDLRRYGSVPH 532 >At3g62330.1 68416.m07002 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 479 Score = 35.1 bits (77), Expect = 0.026 Identities = 18/61 (29%), Positives = 34/61 (55%), Gaps = 2/61 (3%) Frame = +2 Query: 116 QRHVIRQNKDDTSSGRGAQGVHRSRKRG*RHTSRMWYRSRSQQRKRSN--PARSRREAPS 289 ++ +I KD +G VH+ ++ G +S + +RSRS+ +R++ P+R+R P Sbjct: 155 EKFIIVSGKDRLILRKGVDAVHKVKEDGEMKSSSVSHRSRSRSPRRTSVGPSRARNSEPQ 214 Query: 290 R 292 R Sbjct: 215 R 215 >At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / aspartate--tRNA ligase, putative simialr to aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus norvegicus SP|P15178; contains Pfam profile PF00152 tRNA synthetases class II (D, K and N) Length = 532 Score = 29.9 bits (64), Expect = 1.00 Identities = 21/56 (37%), Positives = 29/56 (51%), Gaps = 1/56 (1%) Frame = -2 Query: 440 TESVDVLMPGVGEIVGGSXRXWDHEEXMEGYKRE-GIDPSPXYWYTDQRKFGSVPH 276 + S DV + G EI+ G+ R D E +E RE GID Y D ++G+ PH Sbjct: 444 SNSFDVFIRGE-EIMSGAQRIHD-PELLEKRARECGIDVKTISTYIDAFRYGAPPH 497 >At1g61290.1 68414.m06908 syntaxin, putative (SYP124) similar to syntaxin-related protein Nt-syr1 GI:4206787 from [Nicotiana tabacum] Length = 303 Score = 28.3 bits (60), Expect = 3.0 Identities = 18/68 (26%), Positives = 36/68 (52%) Frame = +3 Query: 135 KIKTIRRRVEALRACTALGSEGRDTRPGCGTGPGASRGSAQTQPVAAVRHRAELALVRIP 314 ++K I++++EAL A ++R G GPG+S +T V+ + + + + Sbjct: 90 RVKMIKQKLEALEKANA------NSRNVSGCGPGSSTDRTRTSVVSGLGKKLKDLMDSFQ 143 Query: 315 VVRARVDA 338 +RAR++A Sbjct: 144 GLRARMNA 151 >At1g11250.1 68414.m01288 syntaxin, putative (SYP125) similar to syntaxin-related protein At-SYR1 GB:AAD11809 GI:4206789 from [Arabidopsis thaliana] Length = 298 Score = 27.5 bits (58), Expect = 5.3 Identities = 19/71 (26%), Positives = 38/71 (53%) Frame = +3 Query: 123 MLSGKIKTIRRRVEALRACTALGSEGRDTRPGCGTGPGASRGSAQTQPVAAVRHRAELAL 302 M+ ++K I++++EAL A R+ PGC GPG+S ++ V+ + + + + Sbjct: 81 MVLKRVKIIKQKLEALEKANA---NSRNV-PGC--GPGSSTDRTRSSVVSGLGKKLKDLM 134 Query: 303 VRIPVVRARVD 335 +RAR++ Sbjct: 135 DSFQGLRARMN 145 >At5g58610.1 68418.m07345 PHD finger transcription factor, putative Length = 1065 Score = 27.1 bits (57), Expect = 7.0 Identities = 17/42 (40%), Positives = 25/42 (59%), Gaps = 2/42 (4%) Frame = +2 Query: 164 GAQGVHRSRKRG*RHTSR--MWYRSRSQQRKRSNPARSRREA 283 G Q V+RS +RG HTS+ M ++R R ++ RSR+ A Sbjct: 535 GLQNVNRSMRRG--HTSKKLMDIKNRVTSRGKTRVLRSRKRA 574 >At2g31660.1 68415.m03865 importin beta-2 subunit family protein similar to D-Importin 7/RanBP7 [Drosophila melanogaster] GI:7542336; contains Pfam profile PF03810: Importin-beta N-terminal domain Length = 1040 Score = 27.1 bits (57), Expect = 7.0 Identities = 11/33 (33%), Positives = 17/33 (51%) Frame = +2 Query: 218 MWYRSRSQQRKRSNPARSRREAPSRTCAGPYTS 316 +W++ Q+RK PA +RE + C TS Sbjct: 823 LWFQMLQQKRKSGLPANFKREHDKKVCCLGLTS 855 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,353,252 Number of Sequences: 28952 Number of extensions: 143837 Number of successful extensions: 440 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 413 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 436 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 878448512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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