BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0268.Seq (489 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 28 0.87 SPBC19C2.12 |mrpl51||mitochondrial ribosomal protein subunit L51... 26 2.7 SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 6.1 SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom... 25 6.1 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 27.9 bits (59), Expect = 0.87 Identities = 18/46 (39%), Positives = 25/46 (54%) Frame = +2 Query: 2 APTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLPPLRLVFPGV 139 AP PAAP+ P+A P Q ++P A+ V + PP+ V P V Sbjct: 544 APQRPAAPVVPEA-PSVPQRPAVPVVPEALSVPQ--PPVAPVAPEV 586 >SPBC19C2.12 |mrpl51||mitochondrial ribosomal protein subunit L51|Schizosaccharomyces pombe|chr 2|||Manual Length = 145 Score = 26.2 bits (55), Expect = 2.7 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +3 Query: 6 RQCQQHRFHLKHVRGKHNHFRQFFYLGRFKYI 101 ++ Q FH+ + +GKH R ++ GR K I Sbjct: 53 KESQDVEFHVTNRQGKHPLIRAYYNTGREKVI 84 >SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 268 Score = 25.0 bits (52), Expect = 6.1 Identities = 9/28 (32%), Positives = 14/28 (50%) Frame = +3 Query: 150 REQTFTQAWSRKNMPLLRKHIRYSLPSP 233 R + +AW P L H +Y +P+P Sbjct: 118 RRFIYQKAWKSYENPSLSSHRQYQIPTP 145 >SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 437 Score = 25.0 bits (52), Expect = 6.1 Identities = 14/35 (40%), Positives = 22/35 (62%) Frame = +2 Query: 164 YTSLVKEKYAFASKTYSIFLTISVRISVFNKLNTT 268 YT L ++ +K Y I+LT + RIS+ + LNT+ Sbjct: 389 YTGLNPQQVDVLAKQYHIYLTKNGRISI-SGLNTS 422 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,808,710 Number of Sequences: 5004 Number of extensions: 37869 Number of successful extensions: 86 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 82 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 86 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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