BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0268.Seq
(489 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 28 0.87
SPBC19C2.12 |mrpl51||mitochondrial ribosomal protein subunit L51... 26 2.7
SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 6.1
SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom... 25 6.1
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 27.9 bits (59), Expect = 0.87
Identities = 18/46 (39%), Positives = 25/46 (54%)
Frame = +2
Query: 2 APTVPAAPIPPQARPGQAQPLQAVLLPRAVQVHRTLPPLRLVFPGV 139
AP PAAP+ P+A P Q ++P A+ V + PP+ V P V
Sbjct: 544 APQRPAAPVVPEA-PSVPQRPAVPVVPEALSVPQ--PPVAPVAPEV 586
>SPBC19C2.12 |mrpl51||mitochondrial ribosomal protein subunit
L51|Schizosaccharomyces pombe|chr 2|||Manual
Length = 145
Score = 26.2 bits (55), Expect = 2.7
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 6 RQCQQHRFHLKHVRGKHNHFRQFFYLGRFKYI 101
++ Q FH+ + +GKH R ++ GR K I
Sbjct: 53 KESQDVEFHVTNRQGKHPLIRAYYNTGREKVI 84
>SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 268
Score = 25.0 bits (52), Expect = 6.1
Identities = 9/28 (32%), Positives = 14/28 (50%)
Frame = +3
Query: 150 REQTFTQAWSRKNMPLLRKHIRYSLPSP 233
R + +AW P L H +Y +P+P
Sbjct: 118 RRFIYQKAWKSYENPSLSSHRQYQIPTP 145
>SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 437
Score = 25.0 bits (52), Expect = 6.1
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +2
Query: 164 YTSLVKEKYAFASKTYSIFLTISVRISVFNKLNTT 268
YT L ++ +K Y I+LT + RIS+ + LNT+
Sbjct: 389 YTGLNPQQVDVLAKQYHIYLTKNGRISI-SGLNTS 422
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,808,710
Number of Sequences: 5004
Number of extensions: 37869
Number of successful extensions: 86
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 82
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 86
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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