BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0264.Seq (399 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family prote... 32 0.16 At1g69280.1 68414.m07943 expressed protein 31 0.37 At3g61670.1 68416.m06911 expressed protein weak similarity to ex... 29 0.86 At1g53130.1 68414.m06016 stigma-specific Stig1 family protein si... 28 2.0 At1g05890.1 68414.m00617 zinc finger protein-related contains lo... 27 6.1 At4g30825.1 68417.m04371 pentatricopeptide (PPR) repeat-containi... 26 8.0 >At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family protein low similarity to SP|Q02104 Lipase 1 precursor (EC 3.1.1.3) (Triacylglycerol lipase) {Psychrobacter immobilis}, SP|P27747|ACOC_ALCEU Dihydrolipoamide acetyltransferase component of acetoin cleaving system (EC 2.3.1.12) [Ralstonia eutropha] {Alcaligenes eutrophus}; contains Pfam profile PF00561: hydrolase, alpha/beta fold family Length = 417 Score = 31.9 bits (69), Expect = 0.16 Identities = 11/44 (25%), Positives = 25/44 (56%), Gaps = 3/44 (6%) Frame = -1 Query: 165 CIHKCCYHQMY-WILR-CLSLNQCYWNCRWMI-HFHHDCWNCIH 43 C C +H+++ W+++ C+ + +W + + H HH W+ +H Sbjct: 292 CFIICKHHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMH 335 >At1g69280.1 68414.m07943 expressed protein Length = 400 Score = 30.7 bits (66), Expect = 0.37 Identities = 21/76 (27%), Positives = 30/76 (39%), Gaps = 4/76 (5%) Frame = -1 Query: 249 SWXXRCLNSNQCYWNCRW-MIHFHHDCWNCIHKC-CYHQMYWILRCLSLNQCYWN--CRW 82 SW C N+ C+ CR ++ C + C C+ W C +N C W Sbjct: 301 SWLC-CKNTGPCFSCCRLPSCGYNFFCCKRLKCCPCFSWCRW-------PSCDYNSSCGW 352 Query: 81 MIHFHHDCWNCIHKCC 34 + H CW+C CC Sbjct: 353 LFCCHWSCWSCC--CC 366 >At3g61670.1 68416.m06911 expressed protein weak similarity to extra-large G-protein [Arabidopsis thaliana] GI:3201682 Length = 790 Score = 29.5 bits (63), Expect = 0.86 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = -1 Query: 189 HFHHDCWNCIHKCCYHQMYW 130 HFHH +C H CY YW Sbjct: 322 HFHHSSCSCYH--CYDNKYW 339 Score = 27.9 bits (59), Expect = 2.6 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -2 Query: 302 HFHHDSXNCLHESXNRGFPGSXAV 231 HFHH S +C H N+ + GS V Sbjct: 322 HFHHSSCSCYHCYDNKYWRGSAPV 345 Score = 27.5 bits (58), Expect = 3.5 Identities = 9/20 (45%), Positives = 11/20 (55%) Frame = -1 Query: 75 HFHHDCWNCIHKCCYRQMFW 16 HFHH +C H CY +W Sbjct: 322 HFHHSSCSCYH--CYDNKYW 339 >At1g53130.1 68414.m06016 stigma-specific Stig1 family protein similar to stigma-specific protein STIG1 [Nicotiana tabacum] GI:496647; contains Pfam profile PF04885: Stigma-specific protein, Stig1 Length = 168 Score = 28.3 bits (60), Expect = 2.0 Identities = 17/60 (28%), Positives = 26/60 (43%) Frame = -1 Query: 216 CYWNCRWMIHFHHDCWNCIHKCCYHQMYWILRCLSLNQCYWNCRWMIHFHHDCWNCIHKC 37 C +CR ++ ++C C HKC + Q RC C C ++ + C C KC Sbjct: 105 CKKHCRNVLGDRNNCGRCGHKCGFGQ-----RC-----CGGVCTYVNFNPNHCGKCTRKC 154 >At1g05890.1 68414.m00617 zinc finger protein-related contains low similarity to zinc finger proteins and Pfam PF01485: IBR domain Length = 552 Score = 26.6 bits (56), Expect = 6.1 Identities = 23/86 (26%), Positives = 33/86 (38%), Gaps = 7/86 (8%) Frame = -1 Query: 249 SWXXRCLNSNQCYWNCRWMIHFHHDC-----WNCIHKCCYHQMYWILRCLSLNQCYWNCR 85 S+ CL S+ WNC H DC W + M WIL + C C+ Sbjct: 238 SYDVSCLCSHSFCWNCTEEAHRPVDCDTVGKWILKNSAESENMNWIL--ANSKPCP-KCK 294 Query: 84 WMIHFHHDCWN--CIHKCCYRQMFWI 13 I +H C + C C + + W+ Sbjct: 295 RPIEKNHGCMHMTCTPPCKF-EFCWL 319 >At4g30825.1 68417.m04371 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 904 Score = 26.2 bits (55), Expect = 8.0 Identities = 10/31 (32%), Positives = 17/31 (54%), Gaps = 1/31 (3%) Frame = -1 Query: 111 LNQCYWNCRWM-IHFHHDCWNCIHKCCYRQM 22 L Y+ R IH++ + +NC+ CC R + Sbjct: 648 LQHLYYRIRKSGIHWNQEMYNCVINCCARAL 678 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 6,045,876 Number of Sequences: 28952 Number of extensions: 107519 Number of successful extensions: 240 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 183 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 238 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 575830496 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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