BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0262.Seq
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g00100.1 68417.m00010 40S ribosomal protein S13 (RPS13A) simi... 142 1e-34
At3g60770.1 68416.m06798 40S ribosomal protein S13 (RPS13A) AtRP... 142 1e-34
At1g65720.1 68414.m07459 expressed protein 32 0.25
At5g50280.1 68418.m06226 pentatricopeptide (PPR) repeat-containi... 30 1.00
At4g17570.1 68417.m02627 zinc finger (GATA type) family protein 28 4.0
At2g28340.1 68415.m03444 zinc finger (GATA type) family protein ... 28 4.0
At5g43930.1 68418.m05374 transducin family protein / WD-40 repea... 27 7.0
At5g20300.1 68418.m02416 chloroplast outer membrane protein, put... 27 7.0
At2g38000.1 68415.m04664 chaperone protein dnaJ-related weak sim... 27 7.0
At2g04160.1 68415.m00400 subtilisin-like protease (AIR3) almost ... 27 7.0
At5g25980.2 68418.m03091 glycosyl hydrolase family 1 protein con... 27 9.3
At5g25980.1 68418.m03090 glycosyl hydrolase family 1 protein con... 27 9.3
>At4g00100.1 68417.m00010 40S ribosomal protein S13 (RPS13A) similar
to ribosomal protein S13; PF00312 (View Sanger Pfam):
ribosomal protein S15; identical to cDNA AtRPS13A mRNA
for cytoplasmic ribosomal protein S13 GI:6521011
Length = 151
Score = 142 bits (344), Expect = 1e-34
Identities = 68/82 (82%), Positives = 74/82 (90%)
Frame = -2
Query: 261 KDLRIMKAMGLAPDLPEDLYYLIKKAVAMRKHLERNRKDKDSKFRLILVESRIHRLARYY 82
K LRI+KA GLAP++PEDLY+LIKKAVA+RKHLERNRKDKDSKFRLILVESRIHRLARYY
Sbjct: 70 KILRILKAHGLAPEIPEDLYHLIKKAVAIRKHLERNRKDKDSKFRLILVESRIHRLARYY 129
Query: 81 KTKSVLPPNWKYESSTASALVA 16
K LPP WKYES+TAS LVA
Sbjct: 130 KKTKKLPPVWKYESTTASTLVA 151
Score = 98.7 bits (235), Expect = 2e-21
Identities = 46/67 (68%), Positives = 53/67 (79%)
Frame = -3
Query: 455 HAPGKGISQSALPNRRSVPTWLKLTADDVKEQIYKLGKKGLTPSQIGVMLRDSHGVAQVR 276
H+ GKGIS SALP +RS P+WLK T+ DV E I K KKGLTPSQIGV+LRDSHG+ QV+
Sbjct: 5 HSRGKGISASALPYKRSSPSWLKTTSQDVDESICKFAKKGLTPSQIGVILRDSHGIPQVK 64
Query: 275 FVTGKKI 255
VTG KI
Sbjct: 65 SVTGSKI 71
>At3g60770.1 68416.m06798 40S ribosomal protein S13 (RPS13A)
AtRPS13A mRNA for cytoplasmic ribosomal protein S13,
Arabidopsis thaliana,AB031739
Length = 151
Score = 142 bits (344), Expect = 1e-34
Identities = 68/82 (82%), Positives = 74/82 (90%)
Frame = -2
Query: 261 KDLRIMKAMGLAPDLPEDLYYLIKKAVAMRKHLERNRKDKDSKFRLILVESRIHRLARYY 82
K LRI+KA GLAP++PEDLY+LIKKAVA+RKHLERNRKDKDSKFRLILVESRIHRLARYY
Sbjct: 70 KILRILKAHGLAPEIPEDLYHLIKKAVAIRKHLERNRKDKDSKFRLILVESRIHRLARYY 129
Query: 81 KTKSVLPPNWKYESSTASALVA 16
K LPP WKYES+TAS LVA
Sbjct: 130 KKTKKLPPVWKYESTTASTLVA 151
Score = 97.9 bits (233), Expect = 3e-21
Identities = 46/67 (68%), Positives = 52/67 (77%)
Frame = -3
Query: 455 HAPGKGISQSALPNRRSVPTWLKLTADDVKEQIYKLGKKGLTPSQIGVMLRDSHGVAQVR 276
H+ GKGIS SALP +RS P+WLK T DV E I K KKGLTPSQIGV+LRDSHG+ QV+
Sbjct: 5 HSRGKGISASALPYKRSSPSWLKTTPQDVDESICKFAKKGLTPSQIGVILRDSHGIPQVK 64
Query: 275 FVTGKKI 255
VTG KI
Sbjct: 65 SVTGSKI 71
>At1g65720.1 68414.m07459 expressed protein
Length = 180
Score = 31.9 bits (69), Expect = 0.25
Identities = 27/79 (34%), Positives = 37/79 (46%)
Frame = +1
Query: 259 FLPVTNLTWATPCESLSITPICEGVRPFFPSL*ICSFTSSAVNFNQVGTLRRLGSADWEI 438
FL +T +T A PC++ I+ + P P+L FTS+ F V T+RRL
Sbjct: 16 FLSITTITTARPCKTFLISSYSLSITPENPNL-ESDFTST--RFVTVFTIRRLN------ 66
Query: 439 PLPGACXPTHVCGFLLNRR 495
P HV F +NRR
Sbjct: 67 -------PHHVVPFFVNRR 78
>At5g50280.1 68418.m06226 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 723
Score = 29.9 bits (64), Expect = 1.00
Identities = 21/85 (24%), Positives = 43/85 (50%)
Frame = -2
Query: 279 KIRNWQKDLRIMKAMGLAPDLPEDLYYLIKKAVAMRKHLERNRKDKDSKFRLILVESRIH 100
++R++++ K M + +P+ Y +A+ K +NRKDK + L ++ S+
Sbjct: 637 RVRDFKRAFFYHKMMVKSGQVPDPRSYEKLRAILEDKAKTKNRKDKTA--ILGIINSKFG 694
Query: 99 RLARYYKTKSVLPPNWKYESSTASA 25
R+ KTK WKY+++ ++
Sbjct: 695 RVKA--KTKGKKDEFWKYKTNRTTS 717
>At4g17570.1 68417.m02627 zinc finger (GATA type) family protein
Length = 510
Score = 27.9 bits (59), Expect = 4.0
Identities = 12/39 (30%), Positives = 15/39 (38%)
Frame = -2
Query: 381 CRRCKGTNLQTWKEGSHSLTNWCNAEGFTWSCPSKIRNW 265
C C TN W+ G CNA G W + N+
Sbjct: 7 CYHCGVTNTPLWRNGPPEKPVLCNACGSRWRTKGTLVNY 45
>At2g28340.1 68415.m03444 zinc finger (GATA type) family protein
and genefinder
Length = 315
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/29 (34%), Positives = 14/29 (48%)
Frame = -2
Query: 387 IDCRRCKGTNLQTWKEGSHSLTNWCNAEG 301
+ C C+ T W+EG + CNA G
Sbjct: 215 LKCTHCETTTTPQWREGPNGRKTLCNACG 243
>At5g43930.1 68418.m05374 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
similar to WD-repeat protein 5 (SP:Q9UGP9) [Homo
sapiens]
Length = 726
Score = 27.1 bits (57), Expect = 7.0
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -2
Query: 366 GTNLQTWKEGSHSLTNWCNAE 304
GTN ++ KE SLT+W AE
Sbjct: 70 GTNNESMKETGQSLTSWVEAE 90
>At5g20300.1 68418.m02416 chloroplast outer membrane protein,
putative similar to chloroplast protein import component
Toc159 [Pisum sativum] GI:8489806, chloroplast outer
envelope protein 86 [Pisum sativum] GI:599958,
GTP-binding protein [Pisum sativum] GI:576509
Length = 793
Score = 27.1 bits (57), Expect = 7.0
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +3
Query: 267 SYESYLGNSM*IPQHYTNL*GSETLLSKFVNL 362
+YESY+G M + QHY + S+T L V L
Sbjct: 308 NYESYVGQRMDVVQHYIHQAVSDTKLENPVLL 339
>At2g38000.1 68415.m04664 chaperone protein dnaJ-related weak
similarity to Chaperone protein dnaJ (Swiss-Prot:Q9ZFC5)
[Methylovorus sp.]
Length = 419
Score = 27.1 bits (57), Expect = 7.0
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = -2
Query: 381 CRRCKGTNLQTWKEGSHSLTNWCNAEGFTWSCPS 280
C C G L K+GS ++ CN +G CP+
Sbjct: 232 CSTCYGRGLVAHKDGSDTICTNCNGKG-KLPCPT 264
>At2g04160.1 68415.m00400 subtilisin-like protease (AIR3) almost
identical to subtilisin-like protease AIR3 GI:4218991
from [Arabidopsis thaliana], missing 200 aa at
N-terminus
Length = 772
Score = 27.1 bits (57), Expect = 7.0
Identities = 22/76 (28%), Positives = 33/76 (43%), Gaps = 7/76 (9%)
Frame = -3
Query: 497 DRRFSKKPQTWVGXHAPGKGISQSALPNRRSVPTWLKL-----TADDVKEQIYKLGKKG- 336
DR F+ G H G+ +S +ALP+ + P + A + Q+ KLG
Sbjct: 353 DREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDP 412
Query: 335 -LTPSQIGVMLRDSHG 291
T +I V LR +G
Sbjct: 413 IKTKGKILVCLRGQNG 428
>At5g25980.2 68418.m03091 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
identical to thioglucosidase (GI:871992) [Arabidopsis
thaliana]; similar to myrosinase precursor (EC
3.2.3.1)(Sinigrinase) (Thioglucosidase) SP|P37702 from
[Arabidopsis thaliana]
Length = 547
Score = 26.6 bits (56), Expect = 9.3
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = -2
Query: 369 KGTNLQTWKEGSHSLTNWCNAE---GFTWSCPSKIRNWQKDLRIMKAMGL 229
+G L W +H A+ G T +C S R WQKDL +M+ +G+
Sbjct: 74 RGRGLNVWDGFTHRYPEKGGADLGNGDT-TCDS-YRTWQKDLDVMEELGV 121
>At5g25980.1 68418.m03090 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
identical to thioglucosidase (GI:871992) [Arabidopsis
thaliana]; similar to myrosinase precursor (EC
3.2.3.1)(Sinigrinase) (Thioglucosidase) SP|P37702 from
[Arabidopsis thaliana]
Length = 471
Score = 26.6 bits (56), Expect = 9.3
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = -2
Query: 369 KGTNLQTWKEGSHSLTNWCNAE---GFTWSCPSKIRNWQKDLRIMKAMGL 229
+G L W +H A+ G T +C S R WQKDL +M+ +G+
Sbjct: 74 RGRGLNVWDGFTHRYPEKGGADLGNGDT-TCDS-YRTWQKDLDVMEELGV 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,239,907
Number of Sequences: 28952
Number of extensions: 199600
Number of successful extensions: 593
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 592
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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