Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0261.Seq
         (499 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1687.06c |rpl44|rpl28|60S ribosomal protein L28/L44|Schizosa...    52   5e-08
SPAC1142.06 |get3||GET complex ATPase subunit Get3 |Schizosaccha...    27   2.1  
SPCC338.13 |cog4||Golgi transport complex subunit Cog4 |Schizosa...    26   3.6  
SPBC14F5.08 |med7||mediator complex subunit Med7|Schizosaccharom...    25   4.8  
SPBC17A3.06 |||phosphoprotein phosphatase|Schizosaccharomyces po...    25   4.8  
SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr 2...    25   4.8  
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ...    25   6.3  
SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory sub...    25   8.4  

>SPAC1687.06c |rpl44|rpl28|60S ribosomal protein
           L28/L44|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 134

 Score = 52.0 bits (119), Expect = 5e-08
 Identities = 26/62 (41%), Positives = 40/62 (64%), Gaps = 1/62 (1%)
 Frame = +1

Query: 73  VKMSSSLNWMIIRNNNAFLVKKRNIKK-PFSKEPNNVTNLHSFRYNGLIHKKAVGVVENP 249
           + +S+ L W +IR+NN FLVK+       F++EP NV+  ++ R++GL + KAVGV  N 
Sbjct: 1   MSVSNDLIWQVIRDNNRFLVKRPEFGGIQFNREPVNVSGKNAQRFSGLCNDKAVGVQANS 60

Query: 250 DR 255
            R
Sbjct: 61  PR 62



 Score = 36.3 bits (80), Expect = 0.003
 Identities = 29/71 (40%), Positives = 37/71 (52%), Gaps = 3/71 (4%)
 Frame = +3

Query: 255 KGFTVVYK-KAKATRKPAKNLIRRPFKAGAR-RSLYK-VKRLLKAXHXRTDLCKAXLRRA 425
           +G  ++ K   K  +KPAK L R+   A A  R  YK +   +     R DL K  + RA
Sbjct: 62  RGVVLITKTNPKNAQKPAK-LFRKDVIANASSRKTYKSIAGRIGRTGYRDDLVKVSVARA 120

Query: 426 SAILRSQRPIK 458
           SAIL SQRP K
Sbjct: 121 SAILSSQRPKK 131


>SPAC1142.06 |get3||GET complex ATPase subunit Get3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 329

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = -1

Query: 52  PNTTCSQCVRRQK 14
           PNTTC QC+ R+K
Sbjct: 264 PNTTCPQCMARRK 276


>SPCC338.13 |cog4||Golgi transport complex subunit Cog4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 738

 Score = 25.8 bits (54), Expect = 3.6
 Identities = 13/37 (35%), Positives = 18/37 (48%)
 Frame = -3

Query: 137 FFTRNALLLRMIIQFSDDDIFTV*TYFYTKHNVLTMC 27
           FFT ++L     +  S   I     Y Y  HN+LT+C
Sbjct: 449 FFTVSSLFFTRFVNESLIPILRNDYYVYLSHNLLTVC 485


>SPBC14F5.08 |med7||mediator complex subunit
           Med7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 376

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 16/64 (25%), Positives = 32/64 (50%)
 Frame = +1

Query: 70  TVKMSSSLNWMIIRNNNAFLVKKRNIKKPFSKEPNNVTNLHSFRYNGLIHKKAVGVVENP 249
           T++   SL++ + +  +     K +IKKP S   +   +   F  N    KK++ ++E+ 
Sbjct: 311 TLEKYKSLDFNMEKEGDVIQQLKSSIKKPLSGAEDEQKSRSMFSKNDEKLKKSLELMEDV 370

Query: 250 DRRD 261
            +RD
Sbjct: 371 IKRD 374


>SPBC17A3.06 |||phosphoprotein phosphatase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 330

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 9/19 (47%), Positives = 15/19 (78%)
 Frame = +2

Query: 236 SWRTLTEGIHSSVQESKGY 292
           +W+   + IHSSVQE++G+
Sbjct: 6   AWQPQFDEIHSSVQEAEGF 24


>SPBC13A2.02 |||nucleoporin Nup82|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 803

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 10/36 (27%), Positives = 21/36 (58%)
 Frame = +1

Query: 85  SSLNWMIIRNNNAFLVKKRNIKKPFSKEPNNVTNLH 192
           S ++ + + NNN ++V + +I+ P+S    +   LH
Sbjct: 415 SQVHSLYLSNNNPYMVLQPDIQSPYSLIAYHANGLH 450


>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 817

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 10/31 (32%), Positives = 19/31 (61%)
 Frame = +2

Query: 230 LVSWRTLTEGIHSSVQESKGYQKAR*KLNPP 322
           L++  TL   +H+++QE KG  +   ++ PP
Sbjct: 453 LIAEETLRRKLHNTIQELKGNIRVFCRVRPP 483


>SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory
           subunit Ekc1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 838

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = -3

Query: 233 PTAFL*IKPLYLKEWRLVTLFGSLLN 156
           PT+F  IKPL  + +R+  L+  LL+
Sbjct: 347 PTSFGKIKPLGFERFRICELYAELLH 372


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,912,482
Number of Sequences: 5004
Number of extensions: 36801
Number of successful extensions: 99
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)