BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0252.Seq
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g24280.1 68418.m02856 expressed protein ; expression supporte... 29 2.3
At3g03010.1 68416.m00296 expressed protein 28 4.0
At2g40300.1 68415.m04964 ferritin, putative similar to ferritin ... 28 4.0
At3g06030.1 68416.m00688 NPK1-related protein kinase, putative (... 27 5.3
At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /... 27 9.3
At1g55350.4 68414.m06326 calpain-type cysteine protease family i... 27 9.3
At1g55350.3 68414.m06325 calpain-type cysteine protease family i... 27 9.3
At1g55350.2 68414.m06324 calpain-type cysteine protease family i... 27 9.3
At1g55350.1 68414.m06323 calpain-type cysteine protease family i... 27 9.3
>At5g24280.1 68418.m02856 expressed protein ; expression supported by
MPSS
Length = 1634
Score = 28.7 bits (61), Expect = 2.3
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -2
Query: 189 IKTSVINDFQSPCIHKCSAENDXALHEKMIXXAFI 85
+ TS+ +D +S C H S E+D + E I AF+
Sbjct: 1155 LDTSIHHDEKSGCFHTMSIESDSSSVESAIRYAFV 1189
>At3g03010.1 68416.m00296 expressed protein
Length = 179
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -1
Query: 310 SSQCIHISYNNYKKLYKKEHSNVNRW 233
++QC H + YKKL ++ +NRW
Sbjct: 84 AAQCSHATLGLYKKLLQRAPKALNRW 109
>At2g40300.1 68415.m04964 ferritin, putative similar to ferritin
subunit cowpea2 precursor [Vigna unguiculata]
GI:2970654; contains Pfam profile PF00210: Ferritin-like
domain
Length = 259
Score = 27.9 bits (59), Expect = 4.0
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = -1
Query: 370 PTKTVESSTRARYSSECRXTSSQCIHISYNNYKKLYKKEHSNVNRWMLAIFG 215
PT + S R +YS EC ++ I++ Y N +Y ++ +R +A+ G
Sbjct: 81 PTSSHLSLARQKYSDECEAAINEQINVEY-NVSYVYHAMYAYFDRDNIALKG 131
>At3g06030.1 68416.m00688 NPK1-related protein kinase, putative
(ANP3) similar to protein kinase [Nicotiana tabacum]
gi|456309|dbj|BAA05648; identical to cDNA NPK1-related
protein kinase 3 GI:2342426
Length = 651
Score = 27.5 bits (58), Expect = 5.3
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -1
Query: 403 NLSRTTSRGTEPTKTVESSTRARYSSEC 320
N SR GTEPT+ ES+ + +S C
Sbjct: 585 NTSRVMQSGTEPTQVNESTKKGVNNSRC 612
>At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh1p (GI:2739044)
{Glycine max}; similar to polyphosphoinositide binding
protein Ssh2, Glycine max, gb:T05953; contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 668
Score = 26.6 bits (56), Expect = 9.3
Identities = 12/38 (31%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -1
Query: 328 SECRXTSSQCIHISYNNYKKLYKKE-HSNVNRWMLAIF 218
+E R + Q +H+ +NY + K+ NV W LA +
Sbjct: 467 TELRLATKQALHLLQDNYPEFVSKQIFINVPWWYLAFY 504
>At1g55350.4 68414.m06326 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 26.6 bits (56), Expect = 9.3
Identities = 13/45 (28%), Positives = 18/45 (40%)
Frame = -3
Query: 464 RWXTSPTTVXXXDPSS*KXKKLVQDNFSWNGTDKDGRKFNQGKIF 330
+W SP V D +D+ W+G GRK G+ F
Sbjct: 1607 QWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDF 1651
>At1g55350.3 68414.m06325 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 26.6 bits (56), Expect = 9.3
Identities = 13/45 (28%), Positives = 18/45 (40%)
Frame = -3
Query: 464 RWXTSPTTVXXXDPSS*KXKKLVQDNFSWNGTDKDGRKFNQGKIF 330
+W SP V D +D+ W+G GRK G+ F
Sbjct: 1607 QWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDF 1651
>At1g55350.2 68414.m06324 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 26.6 bits (56), Expect = 9.3
Identities = 13/45 (28%), Positives = 18/45 (40%)
Frame = -3
Query: 464 RWXTSPTTVXXXDPSS*KXKKLVQDNFSWNGTDKDGRKFNQGKIF 330
+W SP V D +D+ W+G GRK G+ F
Sbjct: 1607 QWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDF 1651
>At1g55350.1 68414.m06323 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 26.6 bits (56), Expect = 9.3
Identities = 13/45 (28%), Positives = 18/45 (40%)
Frame = -3
Query: 464 RWXTSPTTVXXXDPSS*KXKKLVQDNFSWNGTDKDGRKFNQGKIF 330
+W SP V D +D+ W+G GRK G+ F
Sbjct: 1607 QWTDSPPRVDGWDSDPADVDLYDRDDVDWDGQYSSGRKRRSGRDF 1651
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,331,547
Number of Sequences: 28952
Number of extensions: 166799
Number of successful extensions: 362
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 357
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 362
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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