BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0250.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F12.02c |p23fy||translationally controlled tumor protein ho... 46 4e-06 SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 29 0.64 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 2.6 SPBPJ4664.03 |mfm3||M-factor precursor Mfm3|Schizosaccharomyces ... 26 6.0 SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyce... 25 7.9 >SPAC1F12.02c |p23fy||translationally controlled tumor protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 168 Score = 46.4 bits (105), Expect = 4e-06 Identities = 21/46 (45%), Positives = 30/46 (65%) Frame = -1 Query: 389 AKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDGML 252 A+L+E P++V VF+ N +K IL FK+ F+ GESMD D M+ Sbjct: 97 ARLQESNPERVPVFEKNAIGFVKKILANFKDYDFYIGESMDPDAMV 142 Score = 30.3 bits (65), Expect = 0.28 Identities = 16/29 (55%), Positives = 19/29 (65%) Frame = -3 Query: 255 VAMMEYRDFDGTQIPIMMFFKHGLEEEKF 169 V +M YR+ DG P M+FFK GL EKF Sbjct: 142 VVLMNYRE-DGIT-PYMIFFKDGLVSEKF 168 >SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor Ste6|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 29.1 bits (62), Expect = 0.64 Identities = 16/38 (42%), Positives = 22/38 (57%) Frame = +2 Query: 128 ASLELLSYFLSNI*NFSSSRPCLKNIMIGICVPSKSLY 241 AS ELL+ NFS+ R CL+N ++ CVP +Y Sbjct: 781 ASFELLNNLTEARKNFSNYRDCLENCVLP-CVPFLGVY 817 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 27.1 bits (57), Expect = 2.6 Identities = 13/49 (26%), Positives = 23/49 (46%) Frame = -1 Query: 380 EEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDGMLP*WNIE 234 E+ +Q E K+ + K++ EL+ E++D DG N+E Sbjct: 1479 EQITKEQFEQLKSEKERTEKELADSKNELEHLQSEAVDADGKTEISNLE 1527 >SPBPJ4664.03 |mfm3||M-factor precursor Mfm3|Schizosaccharomyces pombe|chr 2|||Manual Length = 41 Score = 25.8 bits (54), Expect = 6.0 Identities = 10/28 (35%), Positives = 17/28 (60%) Frame = +1 Query: 208 DWYLRTIKVSIFHHGNMPSQSIDSPVKN 291 D T+ S+ + GN PS++++ VKN Sbjct: 2 DSMANTVSSSVVNTGNKPSETLNKTVKN 29 >SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 25.4 bits (53), Expect = 7.9 Identities = 9/27 (33%), Positives = 17/27 (62%) Frame = +3 Query: 288 ELKFLKPAEDVFHYFVHVCFKYFNLVR 368 + FLKP ++ YF+ + +Y +L+R Sbjct: 175 QFDFLKPNNALYPYFMRIVQQYTSLIR 201 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,628,785 Number of Sequences: 5004 Number of extensions: 50547 Number of successful extensions: 113 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 109 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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