Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0243.Seq
         (548 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q8T7L8 Cluster: Seroin 1; n=1; Bombyx mori|Rep: Seroin ...   152   4e-36
UniRef50_Q8T7L7 Cluster: Seroin 2; n=1; Bombyx mori|Rep: Seroin ...    48   1e-04
UniRef50_Q08CK1 Cluster: Evolutionarily conserved signaling inte...    36   0.47 
UniRef50_Q21M78 Cluster: PfkB; n=1; Saccharophagus degradans 2-4...    36   0.82 
UniRef50_A5K647 Cluster: Phosphatidylinositol 3-and 4-kinase, pu...    32   7.6  

>UniRef50_Q8T7L8 Cluster: Seroin 1; n=1; Bombyx mori|Rep: Seroin 1 -
           Bombyx mori (Silk moth)
          Length = 108

 Score =  152 bits (369), Expect = 4e-36
 Identities = 73/84 (86%), Positives = 73/84 (86%)
 Frame = +2

Query: 2   FFFVITLITIASAGFVWEDDDDLFPGFSDTFKMPEIPEIKSLEFDDIKTHVAGDNEQYTG 181
           F FVITLITIASAGFVWEDDDDLFPGFSDTFKM EIPEIKSLEFDDIKTHVAGDNEQYTG
Sbjct: 6   FLFVITLITIASAGFVWEDDDDLFPGFSDTFKMREIPEIKSLEFDDIKTHVAGDNEQYTG 65

Query: 182 EXXXXXXXXXTVNGKTVSSGGVSE 253
           E         TVNGKTVSSGGVSE
Sbjct: 66  ESKSSYSSSSTVNGKTVSSGGVSE 89



 Score = 42.7 bits (96), Expect = 0.005
 Identities = 18/19 (94%), Positives = 19/19 (100%)
 Frame = +1

Query: 253 VTNDGKAVEEKVMEYKDGD 309
           +TNDGKAVEEKVMEYKDGD
Sbjct: 90  LTNDGKAVEEKVMEYKDGD 108


>UniRef50_Q8T7L7 Cluster: Seroin 2; n=1; Bombyx mori|Rep: Seroin 2 -
           Bombyx mori (Silk moth)
          Length = 112

 Score = 48.0 bits (109), Expect = 1e-04
 Identities = 24/40 (60%), Positives = 31/40 (77%), Gaps = 1/40 (2%)
 Frame = +2

Query: 2   FFFVITLITIASAGFVWEDDDDLFPGF-SDTFKMPEIPEI 118
           F F+++LITIASAGFVW+DD+  FPGF SD +   +IP I
Sbjct: 6   FLFMLSLITIASAGFVWQDDN--FPGFPSDMWPSIQIPTI 43


>UniRef50_Q08CK1 Cluster: Evolutionarily conserved signaling
           intermediate in Toll pathway, mitochondrial precursor;
           n=8; Euteleostomi|Rep: Evolutionarily conserved
           signaling intermediate in Toll pathway, mitochondrial
           precursor - Danio rerio (Zebrafish) (Brachydanio rerio)
          Length = 452

 Score = 36.3 bits (80), Expect = 0.47
 Identities = 18/72 (25%), Positives = 33/72 (45%)
 Frame = -2

Query: 322 LDLFNRHLCIP*PFLRRLFRHLSLANSAAAHCLAVDRG*GTVTRLRLSGILLVISRDVSF 143
           LD+F + + +P  F++R+F H           L      G +  +    +L+ I  + S 
Sbjct: 146 LDVFPKEVFVPQNFIQRMFNHYPRQQECGVQVLEQMENYGVMPNIETKVLLVQIFGEKSH 205

Query: 142 NIIKFKRLYLWY 107
            I KF+R+  W+
Sbjct: 206 PIRKFQRIMYWF 217


>UniRef50_Q21M78 Cluster: PfkB; n=1; Saccharophagus degradans
           2-40|Rep: PfkB - Saccharophagus degradans (strain 2-40 /
           ATCC 43961 / DSM 17024)
          Length = 318

 Score = 35.5 bits (78), Expect = 0.82
 Identities = 24/89 (26%), Positives = 35/89 (39%)
 Frame = +2

Query: 14  ITLITIASAGFVWEDDDDLFPGFSDTFKMPEIPEIKSLEFDDIKTHVAGDNEQYTGEXXX 193
           + L+T+     +W D DDL       ++  EI E+      D    V GD+EQ       
Sbjct: 195 LALLTLDDEALLWGDSDDLLADAQKRYQSLEISELVFKRGADDVIIVQGDSEQRVSVPKV 254

Query: 194 XXXXXXTVNGKTVSSGGVSE*QMTEKPSK 280
                 T  G T ++G +S     E P K
Sbjct: 255 ENIIDTTAAGDTFNAGYLSARLKGEPPVK 283


>UniRef50_A5K647 Cluster: Phosphatidylinositol 3-and 4-kinase,
           putative; n=4; cellular organisms|Rep:
           Phosphatidylinositol 3-and 4-kinase, putative -
           Plasmodium vivax
          Length = 4367

 Score = 32.3 bits (70), Expect = 7.6
 Identities = 23/76 (30%), Positives = 39/76 (51%)
 Frame = -3

Query: 498 IFAQXSHKITQI*TKKELLRKFDYTMQR*KTLTFYF*QFKIPFCEIIFLT*IFLFVNNRW 319
           +F Q  H  T +  +K LL+K +  +Q  K LT    +  + F   I+   I+  +N   
Sbjct: 137 LFIQHIHVRTFVTLQKFLLQKKENELQ--KLLTLNHLKILLTFLINIYKHKIYEVLNEEQ 194

Query: 318 IYLIAIFVFHDLFFDG 271
           +Y+I+ F+F  +FF G
Sbjct: 195 LYIISKFIFLIIFFFG 210


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 450,958,525
Number of Sequences: 1657284
Number of extensions: 7688327
Number of successful extensions: 16852
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 16503
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16848
length of database: 575,637,011
effective HSP length: 96
effective length of database: 416,537,747
effective search space used: 35822246242
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)