BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0243.Seq (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1D4.07c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 5.6 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 25 7.3 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 9.7 >SPAC1D4.07c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 146 Score = 25.4 bits (53), Expect = 5.6 Identities = 12/34 (35%), Positives = 20/34 (58%) Frame = -2 Query: 541 LIFFTSKTL*MKXLYFRTXQSQNNTNLNKKRTIT 440 +I T KTL + ++F Q++ N NKKR ++ Sbjct: 1 MISKTIKTLIITKIFFSFKQTKRKKNKNKKRILS 34 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 25.0 bits (52), Expect = 7.3 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +2 Query: 77 GFSDTFKMPEIPEIKSLEFDDIKTHVA 157 GFSD ++ E P + +DDI +H A Sbjct: 919 GFSDINEIKEHPFFNGINWDDIFSHEA 945 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 24.6 bits (51), Expect = 9.7 Identities = 14/51 (27%), Positives = 24/51 (47%), Gaps = 4/51 (7%) Frame = +2 Query: 44 FVWEDDDDLFPGFSD--TFKMPEIPEIKSLEFDDIKTHV--AGDNEQYTGE 184 F WED+D+ G+ D + E+ E S F+ ++ DN+ + E Sbjct: 1943 FEWEDEDNASSGYEDELDYNEDEVGENDSTTFEAMENAFTETSDNDDHLEE 1993 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,956,815 Number of Sequences: 5004 Number of extensions: 35063 Number of successful extensions: 69 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 68 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 69 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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