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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0229.Seq
         (419 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B 27a|Schi...    76   2e-15
SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein L27a.2/L28A|Sc...    76   3e-15
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    25   6.3  
SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyce...    25   6.3  
SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc...    24   8.4  
SPBC3B8.06 |||conserved fungal protein|Schizosaccharomyces pombe...    24   8.4  

>SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B
           27a|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 148

 Score = 76.2 bits (179), Expect = 2e-15
 Identities = 37/75 (49%), Positives = 41/75 (54%)
 Frame = +1

Query: 31  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
           M T   KTRKLRGHVS                  AGG  H R + DKYHPGYFGK+GMR 
Sbjct: 1   MPTHTSKTRKLRGHVSAGHGRIGKHRKHPGGRGKAGGLQHLRSHFDKYHPGYFGKVGMRR 60

Query: 211 FHFRKNKNFCPVLNL 255
           FH  KN  + P +NL
Sbjct: 61  FHLMKNPLWRPTVNL 75



 Score = 42.3 bits (95), Expect = 3e-05
 Identities = 22/68 (32%), Positives = 34/68 (50%)
 Frame = +3

Query: 207 KFPL*KEQEFLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK 386
           +F L K   +  +  LD+LWTLV  +TR KY        PVIN++++           P 
Sbjct: 60  RFHLMKNPLWRPTVNLDRLWTLVPNETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPD 119

Query: 387 QPVIXKSK 410
            PVI +++
Sbjct: 120 TPVIIQTR 127


>SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein
           L27a.2/L28A|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 148

 Score = 75.8 bits (178), Expect = 3e-15
 Identities = 37/75 (49%), Positives = 41/75 (54%)
 Frame = +1

Query: 31  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
           M T   KTRKLRGHVS                  AGG  H R + DKYHPGYFGK+GMR 
Sbjct: 1   MPTHVSKTRKLRGHVSAGHGRIGKHRKHPGGRGKAGGLQHLRSHFDKYHPGYFGKVGMRR 60

Query: 211 FHFRKNKNFCPVLNL 255
           FH  KN  + P +NL
Sbjct: 61  FHLMKNPLWRPTVNL 75



 Score = 39.5 bits (88), Expect = 2e-04
 Identities = 20/68 (29%), Positives = 34/68 (50%)
 Frame = +3

Query: 207 KFPL*KEQEFLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK 386
           +F L K   +  +  LD+LWTL+  + R KY        PVIN++++           P+
Sbjct: 60  RFHLMKNPLWRPTVNLDRLWTLLPNEARDKYLGKNTEVAPVINVLQSGYGKVLGKGRLPE 119

Query: 387 QPVIXKSK 410
            PVI +++
Sbjct: 120 TPVIVQTR 127


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
 Frame = +3

Query: 177 SWILW-QTWYEKFPL*KEQEFLSSFELDK 260
           S I+W Q  Y+     +++ F  SFELDK
Sbjct: 75  SGIIWDQELYQNMDYRQDRTFFHSFELDK 103


>SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 406

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 10/20 (50%), Positives = 15/20 (75%)
 Frame = -1

Query: 341 DNIDDGDLAIWSRCILQPRL 282
           DN+D+ DLA +S+ I +P L
Sbjct: 2   DNLDEDDLAFFSKPIKKPPL 21


>SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 940

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 13/42 (30%), Positives = 23/42 (54%)
 Frame = -3

Query: 318 CHLEQMHTSASSVQRLMSKAYQVQNWTEILVLSKVEISHTKF 193
           C +   HT+A+++Q L+S     Q+ +   VLS ++    KF
Sbjct: 382 CAINFPHTAATAIQYLLSHISDFQSKSASSVLSFIKEVMEKF 423


>SPBC3B8.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 511

 Score = 24.2 bits (50), Expect = 8.4
 Identities = 11/23 (47%), Positives = 14/23 (60%)
 Frame = -2

Query: 370 LPSNL*YPALTILMTGTLPSGAD 302
           LPS   +PALT+  TG + S  D
Sbjct: 379 LPSYNVFPALTVFFTGIMMSAHD 401


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,706,886
Number of Sequences: 5004
Number of extensions: 32779
Number of successful extensions: 82
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 78
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 80
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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