BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0229.Seq (419 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B 27a|Schi... 76 2e-15 SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein L27a.2/L28A|Sc... 76 3e-15 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 6.3 SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyce... 25 6.3 SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc... 24 8.4 SPBC3B8.06 |||conserved fungal protein|Schizosaccharomyces pombe... 24 8.4 >SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B 27a|Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 76.2 bits (179), Expect = 2e-15 Identities = 37/75 (49%), Positives = 41/75 (54%) Frame = +1 Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210 M T KTRKLRGHVS AGG H R + DKYHPGYFGK+GMR Sbjct: 1 MPTHTSKTRKLRGHVSAGHGRIGKHRKHPGGRGKAGGLQHLRSHFDKYHPGYFGKVGMRR 60 Query: 211 FHFRKNKNFCPVLNL 255 FH KN + P +NL Sbjct: 61 FHLMKNPLWRPTVNL 75 Score = 42.3 bits (95), Expect = 3e-05 Identities = 22/68 (32%), Positives = 34/68 (50%) Frame = +3 Query: 207 KFPL*KEQEFLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK 386 +F L K + + LD+LWTLV +TR KY PVIN++++ P Sbjct: 60 RFHLMKNPLWRPTVNLDRLWTLVPNETREKYLGKNTEVAPVINVLQSGYGKVLGKGRLPD 119 Query: 387 QPVIXKSK 410 PVI +++ Sbjct: 120 TPVIIQTR 127 >SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein L27a.2/L28A|Schizosaccharomyces pombe|chr 3|||Manual Length = 148 Score = 75.8 bits (178), Expect = 3e-15 Identities = 37/75 (49%), Positives = 41/75 (54%) Frame = +1 Query: 31 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210 M T KTRKLRGHVS AGG H R + DKYHPGYFGK+GMR Sbjct: 1 MPTHVSKTRKLRGHVSAGHGRIGKHRKHPGGRGKAGGLQHLRSHFDKYHPGYFGKVGMRR 60 Query: 211 FHFRKNKNFCPVLNL 255 FH KN + P +NL Sbjct: 61 FHLMKNPLWRPTVNL 75 Score = 39.5 bits (88), Expect = 2e-04 Identities = 20/68 (29%), Positives = 34/68 (50%) Frame = +3 Query: 207 KFPL*KEQEFLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK 386 +F L K + + LD+LWTL+ + R KY PVIN++++ P+ Sbjct: 60 RFHLMKNPLWRPTVNLDRLWTLLPNEARDKYLGKNTEVAPVINVLQSGYGKVLGKGRLPE 119 Query: 387 QPVIXKSK 410 PVI +++ Sbjct: 120 TPVIVQTR 127 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 24.6 bits (51), Expect = 6.3 Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%) Frame = +3 Query: 177 SWILW-QTWYEKFPL*KEQEFLSSFELDK 260 S I+W Q Y+ +++ F SFELDK Sbjct: 75 SGIIWDQELYQNMDYRQDRTFFHSFELDK 103 >SPBC1921.02 |rad60||DNA repair protein Rad60 |Schizosaccharomyces pombe|chr 2|||Manual Length = 406 Score = 24.6 bits (51), Expect = 6.3 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = -1 Query: 341 DNIDDGDLAIWSRCILQPRL 282 DN+D+ DLA +S+ I +P L Sbjct: 2 DNLDEDDLAFFSKPIKKPPL 21 >SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 940 Score = 24.2 bits (50), Expect = 8.4 Identities = 13/42 (30%), Positives = 23/42 (54%) Frame = -3 Query: 318 CHLEQMHTSASSVQRLMSKAYQVQNWTEILVLSKVEISHTKF 193 C + HT+A+++Q L+S Q+ + VLS ++ KF Sbjct: 382 CAINFPHTAATAIQYLLSHISDFQSKSASSVLSFIKEVMEKF 423 >SPBC3B8.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 511 Score = 24.2 bits (50), Expect = 8.4 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = -2 Query: 370 LPSNL*YPALTILMTGTLPSGAD 302 LPS +PALT+ TG + S D Sbjct: 379 LPSYNVFPALTVFFTGIMMSAHD 401 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,706,886 Number of Sequences: 5004 Number of extensions: 32779 Number of successful extensions: 82 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 78 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 80 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 148351622 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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