SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0229.Seq
         (419 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) si...    94   3e-20
At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) id...    91   2e-19
At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) si...    33   0.078
At2g16100.1 68415.m01846 hypothetical protein                          29   1.7  
At5g10770.1 68418.m01252 chloroplast nucleoid DNA-binding protei...    27   5.2  
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi...    26   9.0  

>At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB)
           similar to 60S RIBOSOMAL PROTEIN L27A GB:P49637
           GI:1710530 from [Arabidopsis thaliana]
          Length = 146

 Score = 94.3 bits (224), Expect = 3e-20
 Identities = 43/75 (57%), Positives = 48/75 (64%)
 Frame = +1

Query: 31  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
           MAT+ KK RK RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR 
Sbjct: 1   MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 211 FHFRKNKNFCPVLNL 255
           FH  +NK FCP++NL
Sbjct: 61  FHKLRNKFFCPIVNL 75



 Score = 29.9 bits (64), Expect = 0.73
 Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
 Frame = +3

Query: 234 FLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIXKSK 410
           F     LDKLW+LV E  + K  S+ D  VP+I++ +            P+ +P + K+K
Sbjct: 69  FCPIVNLDKLWSLVPEDVKAK--SSKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVKAK 125

Query: 411 VL 416
           ++
Sbjct: 126 LI 127


>At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC)
           identical to 60S ribosomal protein L27A GB:P49637
           [Arabidopsis thaliana]
          Length = 146

 Score = 91.5 bits (217), Expect = 2e-19
 Identities = 42/75 (56%), Positives = 46/75 (61%)
 Frame = +1

Query: 31  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 210
           M T  KK RK RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR 
Sbjct: 1   MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 211 FHFRKNKNFCPVLNL 255
           FH  +NK FCP++NL
Sbjct: 61  FHKLRNKFFCPIVNL 75



 Score = 29.5 bits (63), Expect = 0.97
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
 Frame = +3

Query: 234 FLSSFELDKLWTLVSEQTRLKYASAPDGKVPVINIVKAXXXXXXXXXXXPK-QPVIXKSK 410
           F     LDKLW+LV E  + K  S  D  VP+I++ +            P+ +P + K+K
Sbjct: 69  FCPIVNLDKLWSLVPEDVKAK--STKD-NVPLIDVTQHGFFKVLGKGHLPENKPFVVKAK 125

Query: 411 VL 416
           ++
Sbjct: 126 LI 127


>At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA)
           similar to GB:BAA96068 from [Panax ginseng]
          Length = 104

 Score = 33.1 bits (72), Expect = 0.078
 Identities = 27/77 (35%), Positives = 34/77 (44%), Gaps = 2/77 (2%)
 Frame = +1

Query: 31  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGK--LGM 204
           M TS+KKTR LR HVS                       H R    +  PG  G   +GM
Sbjct: 1   MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39

Query: 205 RNFHFRKNKNFCPVLNL 255
           R FH  +NK +C ++NL
Sbjct: 40  RYFHKLRNKFYCQIVNL 56


>At2g16100.1 68415.m01846 hypothetical protein 
          Length = 250

 Score = 28.7 bits (61), Expect = 1.7
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +3

Query: 144 APSQNQHGQVPSWILWQTW 200
           +P  +Q  Q+P WILW+ W
Sbjct: 20  SPRTSQFHQLPLWILWRIW 38


>At5g10770.1 68418.m01252 chloroplast nucleoid DNA-binding protein,
           putative similar to CND41, chloroplast nucleoid DNA
           binding protein [Nicotiana tabacum] GI:2541876; contains
           Pfam profile PF00026: Eukaryotic aspartyl protease
          Length = 474

 Score = 27.1 bits (57), Expect = 5.2
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = -2

Query: 316 PSGADAYFSLVCSETNVQSLSSSKLDRNSCS 224
           PS + +Y+++ CS     SLSS+  +  SCS
Sbjct: 177 PSKSTSYYNVSCSSAACGSLSSATGNAGSCS 207


>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase from [Solanum
           tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
           [Hordeum vulgare] GI:21314275, [Oryza sativa]
           GI:3252794; contains Pfam profiles PF00128: Alpha
           amylase catalytic domain, PF02922: Isoamylase N-terminal
           domain
          Length = 783

 Score = 26.2 bits (55), Expect = 9.0
 Identities = 10/25 (40%), Positives = 11/25 (44%)
 Frame = +3

Query: 138 W*APSQNQHGQVPSWILWQTWYEKF 212
           W A    Q G  P W +W  W  KF
Sbjct: 468 WDAGGLYQVGMFPHWGIWSEWNGKF 492


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,086,969
Number of Sequences: 28952
Number of extensions: 181271
Number of successful extensions: 415
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 396
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 414
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -