BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0223.Seq (499 letters) Database: fruitfly 53,049 sequences; 24,988,368 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AY089523-1|AAL90261.1| 143|Drosophila melanogaster GM14585p pro... 167 1e-41 AE013599-1845|AAF58277.2| 143|Drosophila melanogaster CG8415-PA... 167 1e-41 M19494-1|AAA28935.1| 140|Drosophila melanogaster protein ( D.me... 42 6e-04 BT015958-1|AAV36843.1| 140|Drosophila melanogaster RH56553p pro... 42 6e-04 AL133505-2|CAB65841.1| 140|Drosophila melanogaster EG:BACH59J11... 42 6e-04 AL133505-1|CAB63528.1| 154|Drosophila melanogaster EG:BACH59J11... 42 6e-04 AE014298-399|AAF45781.1| 140|Drosophila melanogaster CG7925-PB ... 42 6e-04 AJ250320-1|CAB65721.1| 140|Drosophila melanogaster technical kn... 40 0.002 L11451-1|AAA28918.1| 786|Drosophila melanogaster serine protein... 31 0.87 AY119618-1|AAM50272.1| 683|Drosophila melanogaster LD44584p pro... 31 0.87 AE014297-2154|AAF55277.1| 787|Drosophila melanogaster CG4316-PA... 31 0.87 AE014297-1125|AAF54511.2| 3111|Drosophila melanogaster CG3996-PA... 30 2.0 AE014297-650|AAF54148.1| 358|Drosophila melanogaster CG18268-PA... 28 6.1 DQ138872-1|ABA86478.1| 1228|Drosophila melanogaster CG12234 prot... 28 8.1 AY118535-1|AAM49904.1| 1241|Drosophila melanogaster LD26789p pro... 28 8.1 AF222746-1|AAG53520.1| 1241|Drosophila melanogaster RanBP21 prot... 28 8.1 AE014298-2905|AAF48995.1| 1241|Drosophila melanogaster CG12234-P... 28 8.1 >AY089523-1|AAL90261.1| 143|Drosophila melanogaster GM14585p protein. Length = 143 Score = 167 bits (405), Expect = 1e-41 Identities = 73/79 (92%), Positives = 79/79 (100%) Frame = +1 Query: 1 GKPRGIRTARKHVNHRREQRWADKEFKKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQ 180 GKPRG+RTARKHVNHRR+QRWADK++KKAH+GT+WKANPFGGASHAKGIVLEKVGVEAKQ Sbjct: 2 GKPRGLRTARKHVNHRRDQRWADKDYKKAHLGTRWKANPFGGASHAKGIVLEKVGVEAKQ 61 Query: 181 PNSAIRKCVRVQLIKNGKK 237 PNSAIRKCVRVQLIKNGKK Sbjct: 62 PNSAIRKCVRVQLIKNGKK 80 Score = 103 bits (246), Expect = 2e-22 Identities = 49/58 (84%), Positives = 52/58 (89%) Frame = +3 Query: 252 PRDGCLNHIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLRSLQREKGRPRS 425 PRDG LN+IEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLL + +K RPRS Sbjct: 86 PRDGSLNYIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKEKKERPRS 143 >AE013599-1845|AAF58277.2| 143|Drosophila melanogaster CG8415-PA protein. Length = 143 Score = 167 bits (405), Expect = 1e-41 Identities = 73/79 (92%), Positives = 79/79 (100%) Frame = +1 Query: 1 GKPRGIRTARKHVNHRREQRWADKEFKKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQ 180 GKPRG+RTARKHVNHRR+QRWADK++KKAH+GT+WKANPFGGASHAKGIVLEKVGVEAKQ Sbjct: 2 GKPRGLRTARKHVNHRRDQRWADKDYKKAHLGTRWKANPFGGASHAKGIVLEKVGVEAKQ 61 Query: 181 PNSAIRKCVRVQLIKNGKK 237 PNSAIRKCVRVQLIKNGKK Sbjct: 62 PNSAIRKCVRVQLIKNGKK 80 Score = 103 bits (246), Expect = 2e-22 Identities = 49/58 (84%), Positives = 52/58 (89%) Frame = +3 Query: 252 PRDGCLNHIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLRSLQREKGRPRS 425 PRDG LN+IEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLL + +K RPRS Sbjct: 86 PRDGSLNYIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKEKKERPRS 143 >M19494-1|AAA28935.1| 140|Drosophila melanogaster protein ( D.melanogaster tko (technical knockout) locus mRNA encoding tko gene product,complete cds. ). Length = 140 Score = 41.5 bits (93), Expect = 6e-04 Identities = 22/50 (44%), Positives = 31/50 (62%) Frame = +1 Query: 88 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK 237 H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK+ Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKE 90 >BT015958-1|AAV36843.1| 140|Drosophila melanogaster RH56553p protein. Length = 140 Score = 41.5 bits (93), Expect = 6e-04 Identities = 22/50 (44%), Positives = 31/50 (62%) Frame = +1 Query: 88 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK 237 H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK+ Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKE 90 >AL133505-2|CAB65841.1| 140|Drosophila melanogaster EG:BACH59J11.1,FBgn0003714;tko protein. Length = 140 Score = 41.5 bits (93), Expect = 6e-04 Identities = 22/50 (44%), Positives = 31/50 (62%) Frame = +1 Query: 88 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK 237 H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK+ Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKE 90 >AL133505-1|CAB63528.1| 154|Drosophila melanogaster EG:BACH59J11.1,FBgn0003714;tko protein. Length = 154 Score = 41.5 bits (93), Expect = 6e-04 Identities = 22/50 (44%), Positives = 31/50 (62%) Frame = +1 Query: 88 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK 237 H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK+ Sbjct: 56 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKE 104 >AE014298-399|AAF45781.1| 140|Drosophila melanogaster CG7925-PB protein. Length = 140 Score = 41.5 bits (93), Expect = 6e-04 Identities = 22/50 (44%), Positives = 31/50 (62%) Frame = +1 Query: 88 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK 237 H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK+ Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKE 90 >AJ250320-1|CAB65721.1| 140|Drosophila melanogaster technical knockout protein protein. Length = 140 Score = 39.5 bits (88), Expect = 0.002 Identities = 19/43 (44%), Positives = 27/43 (62%) Frame = +1 Query: 88 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQ 216 H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+ Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVR 84 >L11451-1|AAA28918.1| 786|Drosophila melanogaster serine proteinase protein. Length = 786 Score = 31.1 bits (67), Expect = 0.87 Identities = 23/55 (41%), Positives = 28/55 (50%) Frame = -1 Query: 388 RRDTLATFTTLKRTPGMSPTA*PLRPNPATSTSSFSSMWFRQPSRGRMRSLSFRS 224 RR T T TT +RT PT RP ATS+SS S+ + P+ R S S S Sbjct: 423 RRTTTPT-TTTRRTTTNKPTRPYQRPTTATSSSSTSTTSSKTPTTTRPISSSSSS 476 >AY119618-1|AAM50272.1| 683|Drosophila melanogaster LD44584p protein. Length = 683 Score = 31.1 bits (67), Expect = 0.87 Identities = 23/55 (41%), Positives = 28/55 (50%) Frame = -1 Query: 388 RRDTLATFTTLKRTPGMSPTA*PLRPNPATSTSSFSSMWFRQPSRGRMRSLSFRS 224 RR T T TT +RT PT RP ATS+SS S+ + P+ R S S S Sbjct: 320 RRTTTPT-TTTRRTTTNKPTRPYQRPTTATSSSSTSTTSSKTPTTTRPISSSSSS 373 >AE014297-2154|AAF55277.1| 787|Drosophila melanogaster CG4316-PA protein. Length = 787 Score = 31.1 bits (67), Expect = 0.87 Identities = 23/55 (41%), Positives = 28/55 (50%) Frame = -1 Query: 388 RRDTLATFTTLKRTPGMSPTA*PLRPNPATSTSSFSSMWFRQPSRGRMRSLSFRS 224 RR T T TT +RT PT RP ATS+SS S+ + P+ R S S S Sbjct: 424 RRTTTPT-TTTRRTTTNKPTRPYQRPTTATSSSSTSTTSSKTPTTTRPISSSSSS 477 >AE014297-1125|AAF54511.2| 3111|Drosophila melanogaster CG3996-PA protein. Length = 3111 Score = 29.9 bits (64), Expect = 2.0 Identities = 18/41 (43%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = -1 Query: 352 RTPGMSPTA*PLRPNPATSTSSFSSMW-FRQPSRGRMRSLS 233 +TP SPT P+ +P TS S S+ + PSR R RS S Sbjct: 2256 KTPHTSPTRKPIIKSPYTSPSRKSAKSPYTSPSRNRQRSPS 2296 >AE014297-650|AAF54148.1| 358|Drosophila melanogaster CG18268-PA protein. Length = 358 Score = 28.3 bits (60), Expect = 6.1 Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 2/39 (5%) Frame = +1 Query: 115 PFGGASHAKGIVLEKVGVEAK--QPNSAIRKCVRVQLIK 225 PFG + +VLE+ G K Q IRKCV + +K Sbjct: 123 PFGNVEYVLKVVLERRGTHNKCFQQRLVIRKCVELADLK 161 >DQ138872-1|ABA86478.1| 1228|Drosophila melanogaster CG12234 protein. Length = 1228 Score = 27.9 bits (59), Expect = 8.1 Identities = 13/34 (38%), Positives = 16/34 (47%) Frame = -3 Query: 143 PFA*DAPPKGLAFHFVPMWAFLNSLSAHRCSRRW 42 PF PP VP++A L L R +RRW Sbjct: 910 PFVYSCPPSFYDSVLVPLFAHLAPLMCERLTRRW 943 >AY118535-1|AAM49904.1| 1241|Drosophila melanogaster LD26789p protein. Length = 1241 Score = 27.9 bits (59), Expect = 8.1 Identities = 13/34 (38%), Positives = 16/34 (47%) Frame = -3 Query: 143 PFA*DAPPKGLAFHFVPMWAFLNSLSAHRCSRRW 42 PF PP VP++A L L R +RRW Sbjct: 917 PFVYSCPPSFYDSVLVPLFAHLAPLMCERLTRRW 950 >AF222746-1|AAG53520.1| 1241|Drosophila melanogaster RanBP21 protein. Length = 1241 Score = 27.9 bits (59), Expect = 8.1 Identities = 13/34 (38%), Positives = 16/34 (47%) Frame = -3 Query: 143 PFA*DAPPKGLAFHFVPMWAFLNSLSAHRCSRRW 42 PF PP VP++A L L R +RRW Sbjct: 917 PFVYSCPPSFYDSVLVPLFAHLAPLMCERLTRRW 950 >AE014298-2905|AAF48995.1| 1241|Drosophila melanogaster CG12234-PA protein. Length = 1241 Score = 27.9 bits (59), Expect = 8.1 Identities = 13/34 (38%), Positives = 16/34 (47%) Frame = -3 Query: 143 PFA*DAPPKGLAFHFVPMWAFLNSLSAHRCSRRW 42 PF PP VP++A L L R +RRW Sbjct: 917 PFVYSCPPSFYDSVLVPLFAHLAPLMCERLTRRW 950 Database: fruitfly Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 24,988,368 Number of sequences in database: 53,049 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,973,568 Number of Sequences: 53049 Number of extensions: 497101 Number of successful extensions: 1640 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 1415 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1640 length of database: 24,988,368 effective HSP length: 80 effective length of database: 20,744,448 effective search space used: 1763278080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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