Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0223.Seq
(499 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02960.1 68418.m00239 40S ribosomal protein S23 (RPS23B) ribo... 121 3e-28
At3g09680.1 68416.m01147 40S ribosomal protein S23 (RPS23A) simi... 112 1e-25
At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR... 30 0.75
At1g32960.1 68414.m04059 subtilase family protein contains simil... 28 4.0
At1g32940.1 68414.m04057 subtilase family protein contains simil... 28 4.0
At3g15800.1 68416.m02000 glycosyl hydrolase family 17 protein si... 27 5.3
At4g10550.1 68417.m01727 subtilase family protein contains simil... 27 9.3
>At5g02960.1 68418.m00239 40S ribosomal protein S23 (RPS23B)
ribosomal protein S23, Fragaria x ananassa, PIR:S56673
Length = 142
Score = 121 bits (291), Expect = 3e-28
Identities = 55/79 (69%), Positives = 64/79 (81%)
Frame = +1
Query: 1 GKPRGIRTARKHVNHRREQRWADKEFKKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQ 180
GK RG+ RK R QRWADK++KK+H+G +WK PF G+SHAKGIVLEK+G+EAKQ
Sbjct: 2 GKTRGMGAGRKLKRLRINQRWADKQYKKSHLGNEWK-KPFAGSSHAKGIVLEKIGIEAKQ 60
Query: 181 PNSAIRKCVRVQLIKNGKK 237
PNSAIRKC RVQLIKNGKK
Sbjct: 61 PNSAIRKCARVQLIKNGKK 79
Score = 100 bits (239), Expect = 6e-22
Identities = 45/58 (77%), Positives = 51/58 (87%)
Frame = +3
Query: 252 PRDGCLNHIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLRSLQREKGRPRS 425
P DGCLN+IEENDEVL+AGFGRKGHAVGDIPGVRFKVVKV+ VSLL + +K +PRS
Sbjct: 85 PNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS 142
>At3g09680.1 68416.m01147 40S ribosomal protein S23 (RPS23A) similar
to 40S ribosomal protein S23 (S12) GB:P46297 from
[Fragaria x ananassa]
Length = 142
Score = 112 bits (269), Expect = 1e-25
Identities = 53/79 (67%), Positives = 61/79 (77%)
Frame = +1
Query: 1 GKPRGIRTARKHVNHRREQRWADKEFKKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQ 180
GK RG+ RK R QRWADK +KK++ G +WK PF +SHAKGIVLEK+G+EAKQ
Sbjct: 2 GKTRGMGAGRKLKQLRITQRWADKHYKKSNRGNEWK-KPFACSSHAKGIVLEKIGIEAKQ 60
Query: 181 PNSAIRKCVRVQLIKNGKK 237
PNSAIRKC RVQLIKNGKK
Sbjct: 61 PNSAIRKCARVQLIKNGKK 79
Score = 100 bits (239), Expect = 6e-22
Identities = 45/58 (77%), Positives = 51/58 (87%)
Frame = +3
Query: 252 PRDGCLNHIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLRSLQREKGRPRS 425
P DGCLN+IEENDEVL+AGFGRKGHAVGDIPGVRFKVVKV+ VSLL + +K +PRS
Sbjct: 85 PNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSGVSLLALFKEKKEKPRS 142
>At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 278
Score = 30.3 bits (65), Expect = 0.75
Identities = 25/65 (38%), Positives = 32/65 (49%), Gaps = 1/65 (1%)
Frame = -1
Query: 418 GRPFSLCRERRRD-TLATFTTLKRTPGMSPTA*PLRPNPATSTSSFSSMWFRQPSRGRMR 242
GR +S R R R + + + R+ SP A LR +PA STS R SR + R
Sbjct: 205 GRSYSKSRSRGRSPSRSRSRSRSRSKSRSPKAKSLRRSPAKSTSRSP----RSRSRSKSR 260
Query: 241 SLSFR 227
SLS R
Sbjct: 261 SLSPR 265
>At1g32960.1 68414.m04059 subtilase family protein contains
similarity to subtilase; SP1 GI:9957714 [Oryza sativa]
Length = 777
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 61 WADKEFKKAHMGTKWKANPFGGASHAKG 144
W+ F+ A + T W+ +PFG A+G
Sbjct: 575 WSPAAFRSAIVTTAWRTDPFGEQIFAEG 602
>At1g32940.1 68414.m04057 subtilase family protein contains
similarity to subtilase; SP1 GI:9957714 from [Oryza
sativa]
Length = 774
Score = 27.9 bits (59), Expect = 4.0
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +1
Query: 61 WADKEFKKAHMGTKWKANPFGGASHAKG 144
W+ F+ A + T W+ +PFG A+G
Sbjct: 572 WSPAAFRSAIVTTAWRTDPFGEQIFAEG 599
>At3g15800.1 68416.m02000 glycosyl hydrolase family 17 protein
similar to elicitor inducible chitinase Nt-SubE76
GI:11071974 from [Nicotiana tabacum]
Length = 399
Score = 27.5 bits (58), Expect = 5.3
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = +3
Query: 306 GFGRKGHAVGDIPGVRFKVVKVANVSLLRSLQREKGRP 419
G+ KG A D PG K + N +L + LQ+ KG P
Sbjct: 284 GWASKGDA--DEPGASVKNARTYNRNLRKRLQKRKGTP 319
>At4g10550.1 68417.m01727 subtilase family protein contains
similarity to subtilisin-like protease AIR3 GI:4218991
from [Arabidopsis thaliana]
Length = 778
Score = 26.6 bits (56), Expect = 9.3
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = +1
Query: 61 WADKEFKKAHMGTKWKANPFGGASHAKG 144
W+ + A + T WK +PFG A+G
Sbjct: 576 WSPAAIRSAIVTTAWKTDPFGEQIFAEG 603
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,035,148
Number of Sequences: 28952
Number of extensions: 231314
Number of successful extensions: 538
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 524
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 536
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 878448512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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