BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0221.Seq
(517 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g61670.1 68416.m06911 expressed protein weak similarity to ex... 29 2.5
At3g03790.2 68416.m00389 ankyrin repeat family protein / regulat... 29 2.5
At3g03790.1 68416.m00388 ankyrin repeat family protein / regulat... 29 2.5
At1g69280.1 68414.m07943 expressed protein 29 2.5
At2g04940.1 68415.m00516 scramblase-related weak similarity to P... 28 3.2
At2g29850.1 68415.m03625 hypothetical protein 27 7.5
At4g36080.1 68417.m05136 FAT domain-containing protein / phospha... 27 9.9
>At3g61670.1 68416.m06911 expressed protein weak similarity to
extra-large G-protein [Arabidopsis thaliana] GI:3201682
Length = 790
Score = 28.7 bits (61), Expect = 2.5
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = -2
Query: 60 HFHHDCWNCIHKCCYRXMYW 1
HFHH +C H CY YW
Sbjct: 322 HFHHSSCSCYH--CYDNKYW 339
>At3g03790.2 68416.m00389 ankyrin repeat family protein / regulator
of chromosome condensation (RCC1) family protein similar
to hect domain and RLD 2 GB:NP_004658 [Homo sapiens];
contains Pfam PF00415: Regulator of chromosome
condensation (RCC1); contains Pfam PF00023: Ankyrin
repeat; similar to rjs (GI:3414809) [Mus musculus];
similar to HERC2 (GI:4079809) [Homo sapiens]
Length = 1081
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 22 ALMDTVPTVVMEVYHPRAVPVTLMQAQTLQ 111
A+ +T VV +YHP P+ L ++QTLQ
Sbjct: 497 AVGETHLLVVGSLYHPAYAPIVLKKSQTLQ 526
>At3g03790.1 68416.m00388 ankyrin repeat family protein / regulator
of chromosome condensation (RCC1) family protein similar
to hect domain and RLD 2 GB:NP_004658 [Homo sapiens];
contains Pfam PF00415: Regulator of chromosome
condensation (RCC1); contains Pfam PF00023: Ankyrin
repeat; similar to rjs (GI:3414809) [Mus musculus];
similar to HERC2 (GI:4079809) [Homo sapiens]
Length = 1078
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 22 ALMDTVPTVVMEVYHPRAVPVTLMQAQTLQ 111
A+ +T VV +YHP P+ L ++QTLQ
Sbjct: 494 AVGETHLLVVGSLYHPAYAPIVLKKSQTLQ 523
>At1g69280.1 68414.m07943 expressed protein
Length = 400
Score = 28.7 bits (61), Expect = 2.5
Identities = 19/75 (25%), Positives = 30/75 (40%), Gaps = 1/75 (1%)
Frame = -2
Query: 240 QRTWWILLWCLHQCYWNCQWP-IHFHHDCLSYIHKCCYRRMYWIL*GLCLHQCYWNCPWM 64
+R W+ C+ C+ P ++ C + KCC W C + +C W+
Sbjct: 298 KRCSWLCCKNTGPCFSCCRLPSCGYNFFCCKRL-KCC-PCFSWCRWPSCDYNS--SCGWL 353
Query: 63 IHFHHDCWNCIHKCC 19
H CW+C CC
Sbjct: 354 FCCHWSCWSCC--CC 366
>At2g04940.1 68415.m00516 scramblase-related weak similarity to
Phospholipid scramblase 1 (PL scramblase 1) (Ca(2
)-dependent phospholipid scramblase 1)
(Transplantability associated protein 1) (TRA1) (NOR1)
(Swiss-Prot:Q9JJ00) [Mus musculus]; weak similarity to
Phospholipid scramblase 4 (PL scramblase 4) (Ca(2
)-dependent phospholipid scramblase 4)
(Swiss-Prot:Q9NRQ2) [Homo sapiens]
Length = 392
Score = 28.3 bits (60), Expect = 3.2
Identities = 11/33 (33%), Positives = 15/33 (45%)
Frame = +1
Query: 289 IHRRFQHKWRSFFSSAVNRKSDAASSEDGFWWW 387
+HRR+ H WR + + A GFW W
Sbjct: 265 VHRRW-HLWRRIYDLYLGNNQFAVVENPGFWNW 296
>At2g29850.1 68415.m03625 hypothetical protein
Length = 284
Score = 27.1 bits (57), Expect = 7.5
Identities = 14/48 (29%), Positives = 21/48 (43%)
Frame = +1
Query: 34 TVPTVVMEVYHPRAVPVTLMQAQTLQDPVHPAVAALMDIAQAIVMEVY 177
T P V + A P +Q T D +P AAL + ++ E+Y
Sbjct: 120 TDPAYYYPVLYSTAAPAATIQWPTSTDFAYPLAAALQTLPDDMLQELY 167
>At4g36080.1 68417.m05136 FAT domain-containing protein /
phosphatidylinositol 3- and 4-kinase family protein
contains Pfam profiles PF00454: Phosphatidylinositol 3-
and 4-kinase, PF02259: FAT domain, PF02260: FATC domain
Length = 3839
Score = 26.6 bits (56), Expect = 9.9
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -2
Query: 300 PSVDVTLPSWLLELYLQCCYQRTW 229
PSV + + LL L CCY TW
Sbjct: 1097 PSVRIPVFEQLLPRLLHCCYGSTW 1120
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,564,629
Number of Sequences: 28952
Number of extensions: 162541
Number of successful extensions: 470
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 470
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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