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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0221.Seq
         (517 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g61670.1 68416.m06911 expressed protein weak similarity to ex...    29   2.5  
At3g03790.2 68416.m00389 ankyrin repeat family protein / regulat...    29   2.5  
At3g03790.1 68416.m00388 ankyrin repeat family protein / regulat...    29   2.5  
At1g69280.1 68414.m07943 expressed protein                             29   2.5  
At2g04940.1 68415.m00516 scramblase-related weak similarity to P...    28   3.2  
At2g29850.1 68415.m03625 hypothetical protein                          27   7.5  
At4g36080.1 68417.m05136 FAT domain-containing protein / phospha...    27   9.9  

>At3g61670.1 68416.m06911 expressed protein weak similarity to
           extra-large G-protein [Arabidopsis thaliana] GI:3201682
          Length = 790

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 10/20 (50%), Positives = 11/20 (55%)
 Frame = -2

Query: 60  HFHHDCWNCIHKCCYRXMYW 1
           HFHH   +C H  CY   YW
Sbjct: 322 HFHHSSCSCYH--CYDNKYW 339


>At3g03790.2 68416.m00389 ankyrin repeat family protein / regulator
           of chromosome condensation (RCC1) family protein similar
           to hect domain and RLD 2 GB:NP_004658 [Homo sapiens];
           contains Pfam PF00415: Regulator of chromosome
           condensation (RCC1); contains Pfam PF00023: Ankyrin
           repeat; similar to rjs (GI:3414809) [Mus musculus];
           similar to  HERC2 (GI:4079809) [Homo sapiens]
          Length = 1081

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +1

Query: 22  ALMDTVPTVVMEVYHPRAVPVTLMQAQTLQ 111
           A+ +T   VV  +YHP   P+ L ++QTLQ
Sbjct: 497 AVGETHLLVVGSLYHPAYAPIVLKKSQTLQ 526


>At3g03790.1 68416.m00388 ankyrin repeat family protein / regulator
           of chromosome condensation (RCC1) family protein similar
           to hect domain and RLD 2 GB:NP_004658 [Homo sapiens];
           contains Pfam PF00415: Regulator of chromosome
           condensation (RCC1); contains Pfam PF00023: Ankyrin
           repeat; similar to rjs (GI:3414809) [Mus musculus];
           similar to  HERC2 (GI:4079809) [Homo sapiens]
          Length = 1078

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +1

Query: 22  ALMDTVPTVVMEVYHPRAVPVTLMQAQTLQ 111
           A+ +T   VV  +YHP   P+ L ++QTLQ
Sbjct: 494 AVGETHLLVVGSLYHPAYAPIVLKKSQTLQ 523


>At1g69280.1 68414.m07943 expressed protein
          Length = 400

 Score = 28.7 bits (61), Expect = 2.5
 Identities = 19/75 (25%), Positives = 30/75 (40%), Gaps = 1/75 (1%)
 Frame = -2

Query: 240 QRTWWILLWCLHQCYWNCQWP-IHFHHDCLSYIHKCCYRRMYWIL*GLCLHQCYWNCPWM 64
           +R  W+       C+  C+ P   ++  C   + KCC     W     C +    +C W+
Sbjct: 298 KRCSWLCCKNTGPCFSCCRLPSCGYNFFCCKRL-KCC-PCFSWCRWPSCDYNS--SCGWL 353

Query: 63  IHFHHDCWNCIHKCC 19
              H  CW+C   CC
Sbjct: 354 FCCHWSCWSCC--CC 366


>At2g04940.1 68415.m00516 scramblase-related weak similarity to
           Phospholipid scramblase 1 (PL scramblase 1) (Ca(2
           )-dependent phospholipid scramblase 1)
           (Transplantability associated protein 1) (TRA1) (NOR1)
           (Swiss-Prot:Q9JJ00) [Mus musculus]; weak similarity to
           Phospholipid scramblase 4 (PL scramblase 4) (Ca(2
           )-dependent phospholipid scramblase 4)
           (Swiss-Prot:Q9NRQ2) [Homo sapiens]
          Length = 392

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 11/33 (33%), Positives = 15/33 (45%)
 Frame = +1

Query: 289 IHRRFQHKWRSFFSSAVNRKSDAASSEDGFWWW 387
           +HRR+ H WR  +   +     A     GFW W
Sbjct: 265 VHRRW-HLWRRIYDLYLGNNQFAVVENPGFWNW 296


>At2g29850.1 68415.m03625 hypothetical protein 
          Length = 284

 Score = 27.1 bits (57), Expect = 7.5
 Identities = 14/48 (29%), Positives = 21/48 (43%)
 Frame = +1

Query: 34  TVPTVVMEVYHPRAVPVTLMQAQTLQDPVHPAVAALMDIAQAIVMEVY 177
           T P     V +  A P   +Q  T  D  +P  AAL  +   ++ E+Y
Sbjct: 120 TDPAYYYPVLYSTAAPAATIQWPTSTDFAYPLAAALQTLPDDMLQELY 167


>At4g36080.1 68417.m05136 FAT domain-containing protein /
            phosphatidylinositol 3- and 4-kinase family protein
            contains Pfam profiles PF00454: Phosphatidylinositol 3-
            and 4-kinase, PF02259: FAT domain, PF02260: FATC domain
          Length = 3839

 Score = 26.6 bits (56), Expect = 9.9
 Identities = 11/24 (45%), Positives = 13/24 (54%)
 Frame = -2

Query: 300  PSVDVTLPSWLLELYLQCCYQRTW 229
            PSV + +   LL   L CCY  TW
Sbjct: 1097 PSVRIPVFEQLLPRLLHCCYGSTW 1120


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,564,629
Number of Sequences: 28952
Number of extensions: 162541
Number of successful extensions: 470
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 470
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 937669760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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