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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0219.Seq
         (558 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pomb...    37   0.002
SPAC3C7.01c ||SPAC732.03c|inositol polyphosphate phosphatase |Sc...    27   1.9  
SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII...    27   2.5  
SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|ch...    26   3.3  
SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||...    26   4.3  
SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dp...    25   5.7  
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |...    25   10.0 

>SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 557

 Score = 37.1 bits (82), Expect = 0.002
 Identities = 21/51 (41%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
 Frame = -1

Query: 231 PKTNESKYYRYVFEKSFPGQHNFTPYYWMPK--WVQVSDPSARF-IKHYAA 88
           P T E+ +YR +F++ FP Q   T   W+PK  W    DPS R+   H AA
Sbjct: 505 PTTKEAFWYRKLFDEIFPRQCADTVMRWVPKAEWGCPEDPSGRYQAGHVAA 555



 Score = 32.7 bits (71), Expect = 0.038
 Identities = 19/37 (51%), Positives = 23/37 (62%), Gaps = 6/37 (16%)
 Frame = -2

Query: 413 EKHLLRSSFAK---SGL---LPDCVLWRHKEAFSDGV 321
           EK++LR +F     S L   LP  +LWR KE FSDGV
Sbjct: 436 EKYILRKAFDTTHDSSLQPYLPQDILWRQKEQFSDGV 472


>SPAC3C7.01c ||SPAC732.03c|inositol polyphosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 611

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 8/25 (32%), Positives = 18/25 (72%)
 Frame = +1

Query: 208 IFGFIRFRLQSWILYMSSCNLSVVI 282
           ++GFIR ++  +++ ++SC+L   I
Sbjct: 51  LYGFIRLKIYKYVVLVTSCDLHAAI 75


>SPAC144.09c |sfc2||RNA polymerase III transcription factor
           TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 374

 Score = 26.6 bits (56), Expect = 2.5
 Identities = 9/37 (24%), Positives = 17/37 (45%)
 Frame = -2

Query: 242 QDCSRKRMNPNITATYLRNHFQDNIILLRTIGCQNGF 132
           ++C +K   P++   +LR H  +   +    GC   F
Sbjct: 28  EECGKKYSRPSLLEQHLRTHSNERPFVCDYTGCSKAF 64


>SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 587

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 3/31 (9%)
 Frame = +1

Query: 286 FMTENNDFLTEDTPSLNA---SLCRHSTQSG 369
           + T+N+ FL+ DTP L A   +L  + T+SG
Sbjct: 455 YTTQNSYFLSRDTPKLRALVDALRHYDTKSG 485


>SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 404

 Score = 25.8 bits (54), Expect = 4.3
 Identities = 11/23 (47%), Positives = 12/23 (52%)
 Frame = -1

Query: 207 YRYVFEKSFPGQHNFTPYYWMPK 139
           YR  FE+ F G  N   Y W PK
Sbjct: 349 YRPDFEQCFTGDMNGNIYMWSPK 371


>SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dpb2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 594

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
 Frame = +1

Query: 310 LTEDTPSL--NASLCRHSTQSGNNPLFAKLLLNRCFSTP-FWGCVSLGG 447
           L++ +P+L  +AS C        N +++++L N  F TP F G  S  G
Sbjct: 161 LSKQSPTLMASASACTDMLNRRFNVVYSRILRNESFQTPSFSGSFSQTG 209


>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 499

 Score = 24.6 bits (51), Expect = 10.0
 Identities = 9/31 (29%), Positives = 18/31 (58%)
 Frame = +1

Query: 289 MTENNDFLTEDTPSLNASLCRHSTQSGNNPL 381
           + +N D +T++TP + + +  H T S +  L
Sbjct: 125 LVKNTDSITDETPKVLSEIKEHGTISDSKTL 155


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,334,861
Number of Sequences: 5004
Number of extensions: 49106
Number of successful extensions: 131
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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