BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0219.Seq
(558 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pomb... 37 0.002
SPAC3C7.01c ||SPAC732.03c|inositol polyphosphate phosphatase |Sc... 27 1.9
SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII... 27 2.5
SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|ch... 26 3.3
SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||... 26 4.3
SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dp... 25 5.7
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 25 10.0
>SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 557
Score = 37.1 bits (82), Expect = 0.002
Identities = 21/51 (41%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
Frame = -1
Query: 231 PKTNESKYYRYVFEKSFPGQHNFTPYYWMPK--WVQVSDPSARF-IKHYAA 88
P T E+ +YR +F++ FP Q T W+PK W DPS R+ H AA
Sbjct: 505 PTTKEAFWYRKLFDEIFPRQCADTVMRWVPKAEWGCPEDPSGRYQAGHVAA 555
Score = 32.7 bits (71), Expect = 0.038
Identities = 19/37 (51%), Positives = 23/37 (62%), Gaps = 6/37 (16%)
Frame = -2
Query: 413 EKHLLRSSFAK---SGL---LPDCVLWRHKEAFSDGV 321
EK++LR +F S L LP +LWR KE FSDGV
Sbjct: 436 EKYILRKAFDTTHDSSLQPYLPQDILWRQKEQFSDGV 472
>SPAC3C7.01c ||SPAC732.03c|inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 611
Score = 27.1 bits (57), Expect = 1.9
Identities = 8/25 (32%), Positives = 18/25 (72%)
Frame = +1
Query: 208 IFGFIRFRLQSWILYMSSCNLSVVI 282
++GFIR ++ +++ ++SC+L I
Sbjct: 51 LYGFIRLKIYKYVVLVTSCDLHAAI 75
>SPAC144.09c |sfc2||RNA polymerase III transcription factor
TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual
Length = 374
Score = 26.6 bits (56), Expect = 2.5
Identities = 9/37 (24%), Positives = 17/37 (45%)
Frame = -2
Query: 242 QDCSRKRMNPNITATYLRNHFQDNIILLRTIGCQNGF 132
++C +K P++ +LR H + + GC F
Sbjct: 28 EECGKKYSRPSLLEQHLRTHSNERPFVCDYTGCSKAF 64
>SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 587
Score = 26.2 bits (55), Expect = 3.3
Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 3/31 (9%)
Frame = +1
Query: 286 FMTENNDFLTEDTPSLNA---SLCRHSTQSG 369
+ T+N+ FL+ DTP L A +L + T+SG
Sbjct: 455 YTTQNSYFLSRDTPKLRALVDALRHYDTKSG 485
>SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr
2|||Manual
Length = 404
Score = 25.8 bits (54), Expect = 4.3
Identities = 11/23 (47%), Positives = 12/23 (52%)
Frame = -1
Query: 207 YRYVFEKSFPGQHNFTPYYWMPK 139
YR FE+ F G N Y W PK
Sbjct: 349 YRPDFEQCFTGDMNGNIYMWSPK 371
>SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dpb2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 594
Score = 25.4 bits (53), Expect = 5.7
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +1
Query: 310 LTEDTPSL--NASLCRHSTQSGNNPLFAKLLLNRCFSTP-FWGCVSLGG 447
L++ +P+L +AS C N +++++L N F TP F G S G
Sbjct: 161 LSKQSPTLMASASACTDMLNRRFNVVYSRILRNESFQTPSFSGSFSQTG 209
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 24.6 bits (51), Expect = 10.0
Identities = 9/31 (29%), Positives = 18/31 (58%)
Frame = +1
Query: 289 MTENNDFLTEDTPSLNASLCRHSTQSGNNPL 381
+ +N D +T++TP + + + H T S + L
Sbjct: 125 LVKNTDSITDETPKVLSEIKEHGTISDSKTL 155
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,334,861
Number of Sequences: 5004
Number of extensions: 49106
Number of successful extensions: 131
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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