BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0219.Seq (558 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pomb... 37 0.002 SPAC3C7.01c ||SPAC732.03c|inositol polyphosphate phosphatase |Sc... 27 1.9 SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII... 27 2.5 SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|ch... 26 3.3 SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||... 26 4.3 SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dp... 25 5.7 SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 25 10.0 >SPBC119.10 |asn1||asparagine synthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 557 Score = 37.1 bits (82), Expect = 0.002 Identities = 21/51 (41%), Positives = 28/51 (54%), Gaps = 3/51 (5%) Frame = -1 Query: 231 PKTNESKYYRYVFEKSFPGQHNFTPYYWMPK--WVQVSDPSARF-IKHYAA 88 P T E+ +YR +F++ FP Q T W+PK W DPS R+ H AA Sbjct: 505 PTTKEAFWYRKLFDEIFPRQCADTVMRWVPKAEWGCPEDPSGRYQAGHVAA 555 Score = 32.7 bits (71), Expect = 0.038 Identities = 19/37 (51%), Positives = 23/37 (62%), Gaps = 6/37 (16%) Frame = -2 Query: 413 EKHLLRSSFAK---SGL---LPDCVLWRHKEAFSDGV 321 EK++LR +F S L LP +LWR KE FSDGV Sbjct: 436 EKYILRKAFDTTHDSSLQPYLPQDILWRQKEQFSDGV 472 >SPAC3C7.01c ||SPAC732.03c|inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 611 Score = 27.1 bits (57), Expect = 1.9 Identities = 8/25 (32%), Positives = 18/25 (72%) Frame = +1 Query: 208 IFGFIRFRLQSWILYMSSCNLSVVI 282 ++GFIR ++ +++ ++SC+L I Sbjct: 51 LYGFIRLKIYKYVVLVTSCDLHAAI 75 >SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual Length = 374 Score = 26.6 bits (56), Expect = 2.5 Identities = 9/37 (24%), Positives = 17/37 (45%) Frame = -2 Query: 242 QDCSRKRMNPNITATYLRNHFQDNIILLRTIGCQNGF 132 ++C +K P++ +LR H + + GC F Sbjct: 28 EECGKKYSRPSLLEQHLRTHSNERPFVCDYTGCSKAF 64 >SPCC1840.05c |||phosphomannomutase |Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 26.2 bits (55), Expect = 3.3 Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 3/31 (9%) Frame = +1 Query: 286 FMTENNDFLTEDTPSLNA---SLCRHSTQSG 369 + T+N+ FL+ DTP L A +L + T+SG Sbjct: 455 YTTQNSYFLSRDTPKLRALVDALRHYDTKSG 485 >SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 25.8 bits (54), Expect = 4.3 Identities = 11/23 (47%), Positives = 12/23 (52%) Frame = -1 Query: 207 YRYVFEKSFPGQHNFTPYYWMPK 139 YR FE+ F G N Y W PK Sbjct: 349 YRPDFEQCFTGDMNGNIYMWSPK 371 >SPBP8B7.14c |dpb2||DNA polymerase epsilon catalytic subunit b Dpb2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 594 Score = 25.4 bits (53), Expect = 5.7 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 3/49 (6%) Frame = +1 Query: 310 LTEDTPSL--NASLCRHSTQSGNNPLFAKLLLNRCFSTP-FWGCVSLGG 447 L++ +P+L +AS C N +++++L N F TP F G S G Sbjct: 161 LSKQSPTLMASASACTDMLNRRFNVVYSRILRNESFQTPSFSGSFSQTG 209 >SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 499 Score = 24.6 bits (51), Expect = 10.0 Identities = 9/31 (29%), Positives = 18/31 (58%) Frame = +1 Query: 289 MTENNDFLTEDTPSLNASLCRHSTQSGNNPL 381 + +N D +T++TP + + + H T S + L Sbjct: 125 LVKNTDSITDETPKVLSEIKEHGTISDSKTL 155 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,334,861 Number of Sequences: 5004 Number of extensions: 49106 Number of successful extensions: 131 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 131 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 233995432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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