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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0219.Seq
         (558 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g65010.2 68418.m08178 asparagine synthetase 2 (ASN2) identica...    32   0.30 
At4g27430.1 68417.m03942 COP1-interacting protein 7 (CIP7) ident...    31   0.39 
At5g65010.1 68418.m08177 asparagine synthetase 2 (ASN2) identica...    31   0.69 
At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identica...    31   0.69 
At3g47340.2 68416.m05146 asparagine synthetase 1 [glutamine-hydr...    31   0.69 
At3g47340.1 68416.m05145 asparagine synthetase 1 [glutamine-hydr...    31   0.69 
At4g25420.1 68417.m03656 gibberellin 20-oxidase identical to GI:...    30   0.91 
At2g30720.1 68415.m03747 thioesterase family protein similar to ...    29   2.8  
At5g48370.1 68418.m05976 thioesterase family protein similar to ...    28   3.7  
At5g19080.1 68418.m02268 zinc finger (C3HC4-type RING finger) fa...    27   6.4  

>At5g65010.2 68418.m08178 asparagine synthetase 2 (ASN2) identical
           to asparagine synthetase (ASN2) [Arabidopsis thaliana]
           GI:3859536
          Length = 579

 Score = 31.9 bits (69), Expect = 0.30
 Identities = 13/25 (52%), Positives = 16/25 (64%)
 Frame = -1

Query: 246 YPGLQPKTNESKYYRYVFEKSFPGQ 172
           +P   P T E+ YYR +FEK FP Q
Sbjct: 485 FPDNTPLTKEAYYYRTIFEKFFPKQ 509


>At4g27430.1 68417.m03942 COP1-interacting protein 7 (CIP7) identical
            to COP1-Interacting Protein 7 (CIP7) GI:3327868 from
            [Arabidopsis thaliana]
          Length = 1058

 Score = 31.5 bits (68), Expect = 0.39
 Identities = 14/52 (26%), Positives = 27/52 (51%)
 Frame = -2

Query: 329  DGVSSVKKSLFSVIKEITTERLQEDIYNIQDCSRKRMNPNITATYLRNHFQD 174
            D   +VK +  S  K+ITT+   ED+  +Q    K ++P  + T+   + ++
Sbjct: 955  DDQETVKNTSVSEDKQITTKHYSEDVGEVQASQEKPVSPKKSVTFSETNMEE 1006


>At5g65010.1 68418.m08177 asparagine synthetase 2 (ASN2) identical
           to asparagine synthetase (ASN2) [Arabidopsis thaliana]
           GI:3859536
          Length = 578

 Score = 30.7 bits (66), Expect = 0.69
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = -1

Query: 246 YPGLQPKTNESKYYRYVFEKSFP 178
           +P   P T E+ YYR +FEK FP
Sbjct: 485 FPDNTPLTKEAYYYRTIFEKFFP 507


>At5g10240.1 68418.m01189 asparagine synthetase 3 (ASN3) identical
           to asparagine synthetase (ASN3) [Arabidopsis thaliana]
           GI:3859534
          Length = 578

 Score = 30.7 bits (66), Expect = 0.69
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = -1

Query: 246 YPGLQPKTNESKYYRYVFEKSFP 178
           +P   P T E+ YYR +FEK FP
Sbjct: 485 FPDNTPLTKEAYYYRTIFEKFFP 507


>At3g47340.2 68416.m05146 asparagine synthetase 1
           [glutamine-hydrolyzing] / glutamine-dependent asparagine
           synthetase 1 (ASN1) identical to SP|P49078 Asparagine
           synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
           (Glutamine- dependent asparagine synthetase)
           {Arabidopsis thaliana}
          Length = 512

 Score = 30.7 bits (66), Expect = 0.69
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = -1

Query: 255 HIQYPGLQPKTNESKYYRYVFEKSFP 178
           HI +P   P T E+ YYR +FE+ FP
Sbjct: 483 HI-FPHNTPNTKEAYYYRMIFERFFP 507


>At3g47340.1 68416.m05145 asparagine synthetase 1
           [glutamine-hydrolyzing] / glutamine-dependent asparagine
           synthetase 1 (ASN1) identical to SP|P49078 Asparagine
           synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)
           (Glutamine- dependent asparagine synthetase)
           {Arabidopsis thaliana}
          Length = 584

 Score = 30.7 bits (66), Expect = 0.69
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = -1

Query: 255 HIQYPGLQPKTNESKYYRYVFEKSFP 178
           HI +P   P T E+ YYR +FE+ FP
Sbjct: 483 HI-FPHNTPNTKEAYYYRMIFERFFP 507


>At4g25420.1 68417.m03656 gibberellin 20-oxidase identical to
           GI:1109695
          Length = 377

 Score = 30.3 bits (65), Expect = 0.91
 Identities = 17/44 (38%), Positives = 23/44 (52%)
 Frame = -3

Query: 214 QILPLRI*EIISRTT*FYSVLLDAKMGSGFRPFGKVY*TLCGKM 83
           + L  R  + +SR+        DA +G GF+PFGKVY   C  M
Sbjct: 152 ETLSFRFCDDMSRSKSVQDYFCDA-LGHGFQPFGKVYQEYCEAM 194


>At2g30720.1 68415.m03747 thioesterase family protein similar to
           SP|Q9R0X4 48 kDa acyl-CoA thioester hydrolase,
           mitochondrial precursor (EC 3.1.2.-) {Mus musculus};
           contains Pfam profile PF03061: thioesterase family
           protein
          Length = 455

 Score = 28.7 bits (61), Expect = 2.8
 Identities = 11/54 (20%), Positives = 25/54 (46%)
 Frame = +1

Query: 289 MTENNDFLTEDTPSLNASLCRHSTQSGNNPLFAKLLLNRCFSTPFWGCVSLGGI 450
           + + N  L +DT   N+ +C+   ++ +  +F   L+ + F   F    +  G+
Sbjct: 295 LADRNSILIKDTSHENSLICQPQQRNIHGRIFGGFLMRKAFELAFSNAYTFAGV 348


>At5g48370.1 68418.m05976 thioesterase family protein similar to
           SP|Q9R0X4 48 kDa acyl-CoA thioester hydrolase,
           mitochondrial precursor (EC 3.1.2.-) {Mus musculus};
           contains Pfam profile PF03061: thioesterase family
           protein
          Length = 438

 Score = 28.3 bits (60), Expect = 3.7
 Identities = 12/54 (22%), Positives = 26/54 (48%)
 Frame = +1

Query: 289 MTENNDFLTEDTPSLNASLCRHSTQSGNNPLFAKLLLNRCFSTPFWGCVSLGGI 450
           + + N  L +DT   N+ +C+   ++ +  +F   L++R F   F    +  G+
Sbjct: 267 LADRNSILLKDTRLENSLICQPQQRNIHGRIFGGFLMHRAFELAFSTAYTFAGL 320


>At5g19080.1 68418.m02268 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam domain, PF00097: Zinc
           finger, C3HC4 type (RING finger)
          Length = 378

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 17/62 (27%), Positives = 27/62 (43%), Gaps = 6/62 (9%)
 Frame = -1

Query: 252 IQYPGLQPKTNESKYYRYVFEKSFPGQHN------FTPYYWMPKWVQVSDPSARFIKHYA 91
           I Y       ++++YY       F G H+        P Y+ P  V V +P A +++H  
Sbjct: 74  ISYRPYGQNYHQNQYYPQQAPPYFTGYHHNGFNPMMRPVYFGPTPVAVMEPPAPYVEHQT 133

Query: 90  AK 85
           AK
Sbjct: 134 AK 135


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,869,572
Number of Sequences: 28952
Number of extensions: 244523
Number of successful extensions: 568
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 557
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 568
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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