Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0216.Seq
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein L19B|Schizosa... 114 7e-27
SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein L19|Schizosac... 114 7e-27
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 29 0.45
SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 29 0.60
SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr 1||... 27 1.4
SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||... 27 1.8
SPBP23A10.12 |||FRG1 family protein|Schizosaccharomyces pombe|ch... 26 3.2
SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 3.2
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 26 4.2
SPBC2F12.10 |||mitochondrial ribosomal protein subunit L35|Schiz... 26 4.2
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 5.6
SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces ... 25 7.3
>SPCC1682.14 |rpl1902|rpl19-2|60S ribosomal protein
L19B|Schizosaccharomyces pombe|chr 3|||Manual
Length = 193
Score = 114 bits (275), Expect = 7e-27
Identities = 50/74 (67%), Positives = 65/74 (87%)
Frame = +1
Query: 34 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 213
M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQNIRK++KDGLVI+KP +HSR
Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNIRKLVKDGLVIRKPNLMHSR 60
Query: 214 ARVRKNTEARRKGR 255
R+RK A+R GR
Sbjct: 61 FRIRKTHAAKRLGR 74
Score = 103 bits (246), Expect = 2e-23
Identities = 49/106 (46%), Positives = 65/106 (61%)
Frame = +3
Query: 231 HRST*KGSHCGFGKRRGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLYHSLYMK 410
H + G H G+GKR+GTA ARMP +W + KID+HLYH+LY++
Sbjct: 67 HAAKRLGRHTGYGKRKGTAEARMPSTVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLYLE 126
Query: 411 AKGNVFKNKRVLMEYIHRKKAEKARTKMLNRPGEARRN*VKEARKR 548
AKGN FK+KR L+E+I R KAE RTK++ +ARR K AR+R
Sbjct: 127 AKGNTFKHKRALIEHIQRAKAEANRTKLIQEQQDARRARAKAARQR 172
>SPBC56F2.02 |rpl1901|rpl19-1|60S ribosomal protein
L19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 193
Score = 114 bits (275), Expect = 7e-27
Identities = 50/74 (67%), Positives = 65/74 (87%)
Frame = +1
Query: 34 MSSLKLQKRLAASVMRCGKKKVWLDPNEINEIANTNSRQNIRKMIKDGLVIKKPVAVHSR 213
M++L+ QKRLAASV++CGK+KVW+DPNEI+EI+N NSRQN+RK+IKDGLVI+KP +HSR
Sbjct: 1 MANLRTQKRLAASVLKCGKRKVWMDPNEISEISNANSRQNVRKLIKDGLVIRKPNLMHSR 60
Query: 214 ARVRKNTEARRKGR 255
R+RK A+R GR
Sbjct: 61 FRIRKTHAAKRLGR 74
Score = 103 bits (246), Expect = 2e-23
Identities = 49/106 (46%), Positives = 65/106 (61%)
Frame = +3
Query: 231 HRST*KGSHCGFGKRRGTANARMPQKELWXXXXXXXXXXXXXXXTAKKIDRHLYHSLYMK 410
H + G H G+GKR+GTA ARMP +W + KID+HLYH+LY++
Sbjct: 67 HAAKRLGRHTGYGKRKGTAEARMPSAVVWMRRQRVLRRLLRKYRESGKIDKHLYHTLYLE 126
Query: 411 AKGNVFKNKRVLMEYIHRKKAEKARTKMLNRPGEARRN*VKEARKR 548
AKGN FK+KR L+E+I R KAE RTK++ +ARR K AR+R
Sbjct: 127 AKGNTFKHKRALIEHIQRAKAEANRTKLIQEQQDARRARAKAARQR 172
>SPBC12C2.02c |ste20|ste16|sterility protein
Ste20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1309
Score = 29.1 bits (62), Expect = 0.45
Identities = 12/30 (40%), Positives = 21/30 (70%)
Frame = -3
Query: 177 TILDHLTDVLSGVGVCDLIDFIWIQPHLLF 88
+I +H++DV G+ V LI+ + I+P +LF
Sbjct: 351 SICEHVSDVSRGIAVETLIELMIIRPKILF 380
>SPBC28E12.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 356
Score = 28.7 bits (61), Expect = 0.60
Identities = 12/32 (37%), Positives = 19/32 (59%)
Frame = -3
Query: 156 DVLSGVGVCDLIDFIWIQPHLLFTTSHNRGCK 61
+VLS + ++D + PHLL +S N+ CK
Sbjct: 237 EVLSKLPYFHILDKSFFSPHLLLQSSENQACK 268
>SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 285
Score = 27.5 bits (58), Expect = 1.4
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = -3
Query: 357 TSGAIFLKPFVFVPIVPSVAYAHWLYLFSYQSHSAT 250
+SG + LKP + P + W+ F +SHS+T
Sbjct: 193 SSGGLLLKPAGRIGSSPIPGHGFWIESFDNKSHSST 228
>SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 600
Score = 27.1 bits (57), Expect = 1.8
Identities = 16/56 (28%), Positives = 29/56 (51%), Gaps = 3/56 (5%)
Frame = +1
Query: 4 LVCVGSH-RVRMSSLKLQKRLAASVMRCGKK--KVWLDPNEINEIANTNSRQNIRK 162
+V VG + ++ S + + K L S MRCG+K W++ + + NT+ + K
Sbjct: 305 IVLVGKYTHLQDSYISVIKALEHSAMRCGRKLDLQWVEASHLEASTNTSDPLSYHK 360
>SPBP23A10.12 |||FRG1 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 245
Score = 26.2 bits (55), Expect = 3.2
Identities = 11/38 (28%), Positives = 18/38 (47%)
Frame = +1
Query: 16 GSHRVRMSSLKLQKRLAASVMRCGKKKVWLDPNEINEI 129
G +++ K K L +S+ W D NE+NE+
Sbjct: 9 GDKKIQKKKKKNTKSLQSSLESAENDPFWTDANELNEL 46
>SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 3.2
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = -1
Query: 506 WSVKHLRPGLLSLLPVDVLHEHTLVL 429
W + HL GL + L L+EH L L
Sbjct: 57 WQISHLTGGLFNFLVSQALNEHGLDL 82
>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 25.8 bits (54), Expect = 4.2
Identities = 11/32 (34%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
Frame = -2
Query: 472 AFFLWMYSMSTRLFLNTLPFAFM-*SEWYRCL 380
AF LW+YS+ L+L + W++CL
Sbjct: 337 AFVLWVYSLDKELWLQLDMLGVLNHGSWFKCL 368
>SPBC2F12.10 |||mitochondrial ribosomal protein subunit
L35|Schizosaccharomyces pombe|chr 2|||Manual
Length = 370
Score = 25.8 bits (54), Expect = 4.2
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = +3
Query: 396 SLYMKAKGNVFKNKRVLMEYIHRKKAEKARTKMLNRPGE 512
S+Y A+ N +++ L++ K +K + K+L P E
Sbjct: 81 SIYSAAQENAYRSSVKLIQEYSEKVHKKLQAKLLENPSE 119
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.4 bits (53), Expect = 5.6
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +1
Query: 148 QNIRKMIKDGLVIKKPVAVHSRARVRKNTEARRKGRTV 261
+++R +KD + +PV+V S A ++T G +V
Sbjct: 141 ESLRSKLKDAMASTEPVSVDSAAAPSQSTNPEGNGGSV 178
>SPAC3H8.02 |||sec14 cytosolic factor family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 25.0 bits (52), Expect = 7.3
Identities = 16/65 (24%), Positives = 31/65 (47%), Gaps = 11/65 (16%)
Frame = +1
Query: 94 KVWLDPNEINEIANTNSRQNIRKMIKDGLVIKK-----------PVAVHSRARVRKNTEA 240
K WLDP ++++ T + +++++ I ++K+ P + A KN EA
Sbjct: 289 KSWLDPVVVSKVKFTRNYRDLQQYINPDNILKEFGGPNPWRYTYPEPCQNEAEALKNVEA 348
Query: 241 RRKGR 255
R+ R
Sbjct: 349 RKSLR 353
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,352,022
Number of Sequences: 5004
Number of extensions: 48446
Number of successful extensions: 160
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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