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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0201.Seq
         (449 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||...    36   0.002
SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces...    30   0.19 
SPAC1610.04 |mug99||meiotically upregulated gene Mug99|Schizosac...    28   0.58 
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch...    27   1.3  
SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ...    25   4.1  
SPAC1834.06c |pmo25||mo25 family protein Pmo25|Schizosaccharomyc...    25   5.4  
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|...    25   5.4  
SPCC1450.10c |||iron hydrogenase large subunit |Schizosaccharomy...    25   5.4  
SPBP16F5.06 |||ribosome biogenesis protein Nop6|Schizosaccharomy...    25   5.4  
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ...    25   5.4  
SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase |Schizosacchar...    25   7.1  
SPAC3G9.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||...    25   7.1  
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom...    25   7.1  
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom...    25   7.1  
SPAC688.06c |slx4||structure-specific endonuclease subunit |Schi...    24   9.4  
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c...    24   9.4  

>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1158

 Score = 36.3 bits (80), Expect = 0.002
 Identities = 24/94 (25%), Positives = 44/94 (46%)
 Frame = +2

Query: 50  LPQHLEQCRFSSCSAWNTQKLHDSHHRTRNS*LAGESREPTDEEVVQVLLEYRKKFESSI 229
           +P++  Q +F  C   N  ++H  + R  N+    ++ +P    +  + L+Y  KFE  I
Sbjct: 483 IPENNRQ-QFIRCVFHNIMQIHAVNSRLSNALNRTQTLQPVVNTIGDLFLDYVPKFEPFI 541

Query: 230 CRNTLRHDARFSAEMAASTEKNFESVLVLYQDLR 331
                +  A+F  E   ST +NF + +   + LR
Sbjct: 542 KYGANQAIAKFEFEREKSTNRNFANYVHEVERLR 575


>SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 179

 Score = 29.9 bits (64), Expect = 0.19
 Identities = 11/26 (42%), Positives = 14/26 (53%)
 Frame = -1

Query: 161 GSRLQVSCYVCGDASHVASGCSMRYK 84
           G R  ++CY CG   H A  C+M  K
Sbjct: 111 GHRSNMNCYACGSYGHQARDCTMGVK 136



 Score = 29.1 bits (62), Expect = 0.33
 Identities = 15/56 (26%), Positives = 22/56 (39%), Gaps = 1/56 (1%)
 Frame = -1

Query: 263 KTWHRAEGCCDRYCSQTFFDIPAKPEQPPH-PWAPGSRLQVSCYVCGDASHVASGC 99
           +T H+A  C +    +T +            P +P  R    CY CG   H+A  C
Sbjct: 43  QTGHKASECTEPQQEKTCYACGTAGHLVRDCPSSPNPRQGAECYKCGRVGHIARDC 98


>SPAC1610.04 |mug99||meiotically upregulated gene
           Mug99|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 526

 Score = 28.3 bits (60), Expect = 0.58
 Identities = 13/46 (28%), Positives = 25/46 (54%)
 Frame = +2

Query: 176 EEVVQVLLEYRKKFESSICRNTLRHDARFSAEMAASTEKNFESVLV 313
           +E  + LLE  +KFE  +CRN     + +  +  A+ +K   S+++
Sbjct: 450 KEYWKELLEEGRKFERQLCRNVFGRSSFYVQQKTAAEKKEHISLIM 495


>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
           Mok13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2358

 Score = 27.1 bits (57), Expect = 1.3
 Identities = 10/26 (38%), Positives = 15/26 (57%)
 Frame = -1

Query: 119 SHVASGCSMRYKKKNDTAPGAVAGAT 42
           +H   GC+  + KKN   PG V G++
Sbjct: 321 AHSVRGCAATFNKKNFFIPGEVTGSS 346


>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
           complex subunit |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 218

 Score = 25.4 bits (53), Expect = 4.1
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = +2

Query: 152 GESREPTDEEVVQVLLEYRKKF 217
           GE+ +PT +EV+ V++  RK F
Sbjct: 140 GETLDPTGQEVMGVVINMRKGF 161


>SPAC1834.06c |pmo25||mo25 family protein Pmo25|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 329

 Score = 25.0 bits (52), Expect = 5.4
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = -2

Query: 211 FSIFQQNLNNLLIRGLPALACKS 143
           F I+Q NL  LL+R LP L  +S
Sbjct: 68  FQIYQSNLPFLLVRYLPKLEFES 90


>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 732

 Score = 25.0 bits (52), Expect = 5.4
 Identities = 11/36 (30%), Positives = 16/36 (44%)
 Frame = +2

Query: 47  HLPQHLEQCRFSSCSAWNTQKLHDSHHRTRNS*LAG 154
           H+ ++ E+C   S S  N    H  HH   N  + G
Sbjct: 550 HIHKYNEKCDHESISLQNLDNDHHCHHHHENHNMHG 585


>SPCC1450.10c |||iron hydrogenase large subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 538

 Score = 25.0 bits (52), Expect = 5.4
 Identities = 8/27 (29%), Positives = 15/27 (55%)
 Frame = +2

Query: 62  LEQCRFSSCSAWNTQKLHDSHHRTRNS 142
           L+QC    C++W   + H + ++  NS
Sbjct: 151 LQQCAQEFCNSWLQSRAHKNQNQVTNS 177


>SPBP16F5.06 |||ribosome biogenesis protein Nop6|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 478

 Score = 25.0 bits (52), Expect = 5.4
 Identities = 13/39 (33%), Positives = 20/39 (51%)
 Frame = -1

Query: 320 DKEPKRSRSFFPWKLPFLRKTWHRAEGCCDRYCSQTFFD 204
           +K  K++R F+P     L+K W R E   D   +  F+D
Sbjct: 170 NKNTKKTRFFYPLGKNCLQKLWGRVETNMDN--TTAFYD 206


>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 962

 Score = 25.0 bits (52), Expect = 5.4
 Identities = 13/49 (26%), Positives = 27/49 (55%)
 Frame = -2

Query: 223 ALKLFSIFQQNLNNLLIRGLPALACKSAVTCAVMRVM*LLGVPCATRRK 77
           +L+L S F+++L N++ +      C++ +    MRV+  + V C   R+
Sbjct: 454 SLQLQSFFEKHLGNVVDKFFLEHLCETTIESDAMRVVTEMLVSCYENRE 502


>SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 413

 Score = 24.6 bits (51), Expect = 7.1
 Identities = 13/35 (37%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
 Frame = -1

Query: 335 KNEDPDKEPKRSRSF-FPWKLPFLRKTWHRAEGCC 234
           K+E P  E  R ++F   WK P   K   +  GCC
Sbjct: 284 KSELPPWEESRVKTFPLLWKNPVTGKLALQTHGCC 318


>SPAC3G9.01 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 462

 Score = 24.6 bits (51), Expect = 7.1
 Identities = 15/42 (35%), Positives = 18/42 (42%)
 Frame = +3

Query: 60  TWSSVVFLLVAHGTPRSYMTRITAHVTADLQARAGSPRMRRL 185
           T SS  F++V H TP     R      A     A   R+RRL
Sbjct: 242 TSSSKTFVIVDHSTPSPPSIRTKLEAFAPNSTFATQKRLRRL 283


>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
            pombe|chr 1||Partial|Manual
          Length = 1887

 Score = 24.6 bits (51), Expect = 7.1
 Identities = 8/20 (40%), Positives = 11/20 (55%)
 Frame = -1

Query: 143  SCYVCGDASHVASGCSMRYK 84
            +CY CG A H    C +R +
Sbjct: 1805 TCYKCGKADHNLRECKLRIR 1824


>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1919

 Score = 24.6 bits (51), Expect = 7.1
 Identities = 8/20 (40%), Positives = 11/20 (55%)
 Frame = -1

Query: 143  SCYVCGDASHVASGCSMRYK 84
            +CY CG A H    C +R +
Sbjct: 1805 TCYKCGKADHNLRECKLRIR 1824


>SPAC688.06c |slx4||structure-specific endonuclease subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 419

 Score = 24.2 bits (50), Expect = 9.4
 Identities = 14/36 (38%), Positives = 18/36 (50%)
 Frame = +2

Query: 158 SREPTDEEVVQVLLEYRKKFESSICRNTLRHDARFS 265
           +R   D +V+  L  Y K   S+ CRNT  H A  S
Sbjct: 114 TRVAEDNDVL--LSRYSKIKNSASCRNTFEHSAYHS 147


>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 871

 Score = 24.2 bits (50), Expect = 9.4
 Identities = 11/27 (40%), Positives = 12/27 (44%)
 Frame = -1

Query: 200 PAKPEQPPHPWAPGSRLQVSCYVCGDA 120
           PA    PP  W P   L +S Y   DA
Sbjct: 811 PALKATPPSVWIPKDPLGLSTYAIEDA 837


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,927,176
Number of Sequences: 5004
Number of extensions: 37626
Number of successful extensions: 151
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 166231220
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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