Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0197.Seq
(558 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 157 1e-39
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 156 2e-39
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 156 2e-39
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 96 4e-21
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 91 8e-20
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 71 2e-13
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 35 0.009
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 33 0.022
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 33 0.022
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.15
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.20
SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 29 0.35
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.35
SPBC11B10.05c |rsp1||random septum position protein Rsp1|Schizos... 29 0.46
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 29 0.61
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 28 1.1
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 28 1.1
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 27 1.4
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 2.5
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 25 7.5
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 25 7.5
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 25 10.0
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 25 10.0
SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 10.0
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 157 bits (380), Expect = 1e-39
Identities = 73/84 (86%), Positives = 77/84 (91%)
Frame = +2
Query: 2 TIDIXLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIXKNG 181
TIDI LWKFET KY VT+IDAPGHRDFIKNMITGTSQADCAVLI+ GTGEFEAGI K+G
Sbjct: 72 TIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDG 131
Query: 182 QTREHALLAFTLGVKQLIVGVNKM 253
QTREHALLA+TLGVKQLIV VNKM
Sbjct: 132 QTREHALLAYTLGVKQLIVAVNKM 155
Score = 106 bits (254), Expect = 3e-24
Identities = 47/96 (48%), Positives = 61/96 (63%)
Frame = +1
Query: 271 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 450
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 451 KADGKCLIESSRCHPATCPPLTXPCVFPCXTYXKIG 558
GK L+E+ P P P KIG
Sbjct: 220 VVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIG 255
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 156 bits (379), Expect = 2e-39
Identities = 72/84 (85%), Positives = 77/84 (91%)
Frame = +2
Query: 2 TIDIXLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIXKNG 181
TIDI LWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGI K+G
Sbjct: 72 TIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDG 131
Query: 182 QTREHALLAFTLGVKQLIVGVNKM 253
QTREHALLA+TLGVKQLIV VNKM
Sbjct: 132 QTREHALLAYTLGVKQLIVAVNKM 155
Score = 106 bits (254), Expect = 3e-24
Identities = 47/96 (48%), Positives = 61/96 (63%)
Frame = +1
Query: 271 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 450
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 451 KADGKCLIESSRCHPATCPPLTXPCVFPCXTYXKIG 558
GK L+E+ P P P KIG
Sbjct: 220 VVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIG 255
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 156 bits (379), Expect = 2e-39
Identities = 72/84 (85%), Positives = 77/84 (91%)
Frame = +2
Query: 2 TIDIXLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIXKNG 181
TIDI LWKFET KY VT+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEFEAGI K+G
Sbjct: 72 TIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDG 131
Query: 182 QTREHALLAFTLGVKQLIVGVNKM 253
QTREHALLA+TLGVKQLIV VNKM
Sbjct: 132 QTREHALLAYTLGVKQLIVAVNKM 155
Score = 106 bits (254), Expect = 3e-24
Identities = 47/96 (48%), Positives = 61/96 (63%)
Frame = +1
Query: 271 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEG 450
+S+ RFEEI KE S++IKK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K G
Sbjct: 160 WSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAG 219
Query: 451 KADGKCLIESSRCHPATCPPLTXPCVFPCXTYXKIG 558
GK L+E+ P P P KIG
Sbjct: 220 VVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIG 255
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 95.9 bits (228), Expect = 4e-21
Identities = 42/84 (50%), Positives = 59/84 (70%)
Frame = +2
Query: 2 TIDIXLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIXKNG 181
T+++ FET +++DAPGH+ ++ NMI G SQAD VL+++A GEFEAG + G
Sbjct: 303 TVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGG 362
Query: 182 QTREHALLAFTLGVKQLIVGVNKM 253
QTREHA+LA T G+ L+V +NKM
Sbjct: 363 QTREHAVLARTQGINHLVVVINKM 386
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 91.5 bits (217), Expect = 8e-20
Identities = 43/84 (51%), Positives = 54/84 (64%)
Frame = +2
Query: 2 TIDIXLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIXKNG 181
T+D+ FE+ K I DAPGHRDFI MI G S AD AVL+V + FE G +NG
Sbjct: 242 TMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLENG 301
Query: 182 QTREHALLAFTLGVKQLIVGVNKM 253
QTREHA L LG+ +++V VNK+
Sbjct: 302 QTREHAYLLRALGISEIVVSVNKL 325
Score = 45.2 bits (102), Expect = 7e-06
Identities = 24/55 (43%), Positives = 34/55 (61%), Gaps = 3/55 (5%)
Frame = +1
Query: 271 YSEPRFEEIKKEVSSY-IKKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKG 426
+SE RF+EIK VS + IK +G+ + V FVPIS G N++ + S W+KG
Sbjct: 330 WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG 384
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 70.5 bits (165), Expect = 2e-13
Identities = 34/77 (44%), Positives = 51/77 (66%)
Frame = +2
Query: 23 KFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIXKNGQTREHAL 202
++ET+ + +D PGH D+IKNMITG + D A+++V+A G+ QTREH L
Sbjct: 110 EYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQMP-------QTREHLL 162
Query: 203 LAFTLGVKQLIVGVNKM 253
LA +GVKQ++V +NK+
Sbjct: 163 LARQVGVKQIVVYINKV 179
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 34.7 bits (76), Expect = 0.009
Identities = 19/70 (27%), Positives = 33/70 (47%)
Frame = +2
Query: 44 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIXKNGQTREHALLAFTLGV 223
+V+ +D PGH + M+ G + D A+L++A QT EH + +
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQL 162
Query: 224 KQLIVGVNKM 253
K +I+ NK+
Sbjct: 163 KHIIILQNKV 172
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 33.5 bits (73), Expect = 0.022
Identities = 21/57 (36%), Positives = 28/57 (49%)
Frame = +2
Query: 26 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIXKNGQTREH 196
FE S Y + IID PGH DF + D AVL++ A +G I + Q R +
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 33.5 bits (73), Expect = 0.022
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +2
Query: 26 FETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 151
F+ ++T +D PGH F G + AD VL+VA G
Sbjct: 215 FDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAGDDG 256
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 30.7 bits (66), Expect = 0.15
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +2
Query: 17 LWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 151
++ + Y + +ID PGH DF ++ + + +L+V A G
Sbjct: 116 IYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG 160
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.20
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +2
Query: 41 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 151
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 29.5 bits (63), Expect = 0.35
Identities = 27/79 (34%), Positives = 37/79 (46%)
Frame = +2
Query: 134 VAAGTGEFEAGIXKNGQTREHALLAFTLGVKQLIVGVNKMVPLNHHTVSPDLRKSRRKYP 313
VA GT E GI NG E+A+ A +I V++ P++H + KSRR+YP
Sbjct: 47 VAEGTAE---GIVSNG---EYAVGAMGAAPTNIIGSVDQQPPVSHTS-----HKSRRQYP 95
Query: 314 HTSRRLATTQLLSLSCPFL 370
L T S + P L
Sbjct: 96 AEVFELTNTLAASPAPPSL 114
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.35
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 53 IIDAPGHRDFIKNMITGTSQADCAVLIV 136
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPBC11B10.05c |rsp1||random septum position protein
Rsp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 494
Score = 29.1 bits (62), Expect = 0.46
Identities = 26/95 (27%), Positives = 40/95 (42%)
Frame = +1
Query: 232 HRRSKQNGSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKM 411
HR +G E S F+E+ SYI +P +G+ +N+++P +
Sbjct: 143 HRSFSASGKMEKNNS---FKEVSTS-KSYISSGYLHPKTSPIFKKNGYATENVVDPISSS 198
Query: 412 PWFKGWQVERKEGKADGKCLIESSRCHPATCPPLT 516
P FKG K + K +ES R T PL+
Sbjct: 199 PRFKG----PNYNKFNAKLYLESLREKRRTYTPLS 229
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 28.7 bits (61), Expect = 0.61
Identities = 15/50 (30%), Positives = 21/50 (42%)
Frame = +2
Query: 2 TIDIXLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 151
TI+ F + +ID PGH DF + + D AV I+ G
Sbjct: 80 TINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG 129
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 41 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 136
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +2
Query: 41 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 136
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 27.5 bits (58), Expect = 1.4
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = +2
Query: 272 TVSPDLRKSRRKYPHTSRRLATTQLL---SLSCPFLDGTETTCWSLQPKCLGSRDGRWSV 442
+ +PD+ ++PHT +R +T + L LSC + +E + P G +D + +
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRCLSCEYSTLSEASDSMSNPLFFGDQDNGYVI 204
Query: 443 KKA 451
++
Sbjct: 205 SQS 207
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 26.6 bits (56), Expect = 2.5
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2
Query: 56 IDAPGHRDFIKNMITGTSQADCAVLIV 136
ID PGH DF+ + + +D VL+V
Sbjct: 214 IDTPGHVDFVDEVAAPMAISDGVVLVV 240
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/31 (38%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
Frame = +2
Query: 5 IDIXLWKFETSKYYV--TIIDAPGHRDFIKN 91
I+I + E +++ T+ID PG DFI N
Sbjct: 73 IEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.0 bits (52), Expect = 7.5
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +2
Query: 368 LDGTETTCWSLQPKCLGSRDGRWSVKKAKLTENASLK 478
LD + W++QP C + DG A L++NA LK
Sbjct: 149 LDKLKDRLWNVQPTCALTGDGLLE-GLAWLSQNAKLK 184
>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 734
Score = 24.6 bits (51), Expect = 10.0
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = -1
Query: 216 RVKASKACSRV*PFLXIPASNSPVP 142
R S +C+R+ P + +P S P P
Sbjct: 601 RTLQSLSCARIRPLVMVPKSKKPSP 625
>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 835
Score = 24.6 bits (51), Expect = 10.0
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = -2
Query: 479 LSMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 372
+S+ P+ LPS + P L E +ML CH
Sbjct: 555 ISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590
>SPAC1687.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 96
Score = 24.6 bits (51), Expect = 10.0
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -3
Query: 100 CDHVLDEISVSRSINDGNIVLASFEL 23
CDH+L IS SR G + FEL
Sbjct: 23 CDHLLQYISNSRDSKAGLKIFLVFEL 48
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,303,671
Number of Sequences: 5004
Number of extensions: 48216
Number of successful extensions: 183
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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