Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0177.Seq
(518 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 112 3e-26
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 112 3e-26
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 100 1e-22
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 99 5e-22
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 74 1e-14
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 51 9e-08
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 7e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.24
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.55
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 2.2
SPAC23H3.08c |bub3||mitotic spindle checkpoint protein Bub3|Schi... 26 3.9
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 3.9
SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co... 25 5.1
SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|... 25 6.8
SPAC139.06 |hat1|SPAC23C4.01|histone acetyltransferase Hat1|Schi... 25 6.8
SPCC553.06 |||oligosaccharyltransferase subunit|Schizosaccharomy... 25 6.8
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 6.8
SPBC15D4.10c |amo1||nuclear rim protein Amo1|Schizosaccharomyces... 25 6.8
SPAC1A6.01c ||SPAC23C4.20c|human thyroid receptor interacting pr... 25 9.0
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 25 9.0
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 9.0
SPAC2F3.04c |rim1||mitochondrial single-stranded DNA binding pro... 25 9.0
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 112 bits (270), Expect = 3e-26
Identities = 51/59 (86%), Positives = 56/59 (94%)
Frame = +3
Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPN 254
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPH 62
Score = 103 bits (247), Expect = 2e-23
Identities = 49/87 (56%), Positives = 61/87 (70%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P +TIFDAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF P +
Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
L K R+ EA+LG V +AV+T AY
Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAY 147
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 112 bits (270), Expect = 3e-26
Identities = 51/59 (86%), Positives = 56/59 (94%)
Frame = +3
Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPN 254
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPH 62
Score = 110 bits (264), Expect = 1e-25
Identities = 52/87 (59%), Positives = 63/87 (72%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P +TIFDAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF P +
Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
L K R+ EAYLG V +AV+T AY
Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAY 147
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 100 bits (240), Expect = 1e-22
Identities = 47/63 (74%), Positives = 54/63 (85%), Gaps = 1/63 (1%)
Frame = +3
Query: 78 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP-N 254
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67
Query: 255 TQY 263
T +
Sbjct: 68 TVF 70
Score = 91.1 bits (216), Expect = 9e-20
Identities = 43/87 (49%), Positives = 58/87 (66%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P++T+FDAKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F ++
Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
L K +++ EA L K V AVIT AY
Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAY 151
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 98.7 bits (235), Expect = 5e-22
Identities = 45/64 (70%), Positives = 54/64 (84%), Gaps = 1/64 (1%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP-NT 257
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP NT
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 258 QYSM 269
+ +
Sbjct: 98 IFDI 101
Score = 85.0 bits (201), Expect = 6e-18
Identities = 40/87 (45%), Positives = 58/87 (66%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P++TIFD KRLIGRKF++ T+ D+K +PF +V+D +P ++V G+ K F P +
Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
L K ++ EAYLGK V +AV+T AY
Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAY 180
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 73.7 bits (173), Expect = 1e-14
Identities = 34/66 (51%), Positives = 45/66 (68%), Gaps = 1/66 (1%)
Frame = +3
Query: 69 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 245
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q +
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 246 NPNTQY 263
NP +
Sbjct: 109 NPENTF 114
Score = 60.9 bits (141), Expect = 1e-10
Identities = 33/87 (37%), Positives = 48/87 (55%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P++T F KRLIGR+F++ VQ D+K P+++V + KT+ P + +
Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNG---DAWLEARGKTYSPSQIGGF 166
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
L K R+ YLGK V NAV+T AY
Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAY 193
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 51.2 bits (117), Expect = 9e-08
Identities = 21/61 (34%), Positives = 39/61 (63%)
Frame = +3
Query: 66 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 245
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62
Query: 246 N 248
N
Sbjct: 63 N 63
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 41.5 bits (93), Expect = 7e-05
Identities = 17/56 (30%), Positives = 31/56 (55%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 248
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRN 82
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 29.9 bits (64), Expect = 0.24
Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNT 257
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFP-- 82
Query: 258 QYSMPNV 278
+S+ NV
Sbjct: 83 AHSIRNV 89
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.55
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +1
Query: 73 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 168
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 26.6 bits (56), Expect = 2.2
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +3
Query: 177 PSYVAFTDTERLIGDAAKNQVAMNPNTQYSMPNVSSDVSSKMLLC 311
P +A T ER++ AAK A+N Y++ ++ D K++ C
Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296
>SPAC23H3.08c |bub3||mitotic spindle checkpoint protein
Bub3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 320
Score = 25.8 bits (54), Expect = 3.9
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -3
Query: 453 GSFLHFRKHHGTDFLGKKVLSSPL 382
GS LH++ + LGK LSSP+
Sbjct: 35 GSLLHYQISENPELLGKYDLSSPI 58
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.8 bits (54), Expect = 3.9
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = -3
Query: 159 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 1
G W SP S + H ++++ +F +RVL C+ L K +K+A L
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323
>SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 675
Score = 25.4 bits (53), Expect = 5.1
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +3
Query: 222 AAKNQVAMNPNTQYSMPNVSSDVSSKML 305
A K Q+ +NP + +PNV D + +L
Sbjct: 323 ARKRQIQLNPFPPWDVPNVLEDQMNSLL 350
>SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 330
Score = 25.0 bits (52), Expect = 6.8
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -2
Query: 388 TLICYLDLRFASITDNLERPVLHVGLHSSIFELTSDETFGI 266
T I L + S+TDN+ER + +G + I +TS F +
Sbjct: 142 TNIALLLATYPSVTDNIER-FIFMGGSTGIGNITSQAEFNV 181
>SPAC139.06 |hat1|SPAC23C4.01|histone acetyltransferase
Hat1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 25.0 bits (52), Expect = 6.8
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = +3
Query: 339 RLSVMEANLRSR*HIRVKTKPFSRGSQFHGAYENEGNCPKXILAKL 476
RL + E R + ++ R + H AYEN+ + K I+ KL
Sbjct: 314 RLGIKERIFRQNLDVLLQLDKSERIEKIHNAYENQFDEYKQIVKKL 359
>SPCC553.06 |||oligosaccharyltransferase subunit|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 271
Score = 25.0 bits (52), Expect = 6.8
Identities = 9/20 (45%), Positives = 16/20 (80%), Gaps = 1/20 (5%)
Frame = -2
Query: 307 SSIFELTSDETFGIEY-CVL 251
S+++ELT DET +++ C+L
Sbjct: 51 STVYELTGDETLNLKFTCIL 70
>SPAC2F7.03c |pom1||DYRK family protein kinase
Pom1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1087
Score = 25.0 bits (52), Expect = 6.8
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = +2
Query: 59 QNGKSTRSRNRSGYHVLLRWCLPARE 136
+NGK T +R +S + W P++E
Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE 502
>SPBC15D4.10c |amo1||nuclear rim protein Amo1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 475
Score = 25.0 bits (52), Expect = 6.8
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = +3
Query: 246 NPNTQYSMPNVSSDVSSKMLLCKPT*STGLS 338
N NTQ+S+PN S V L +P G +
Sbjct: 327 NLNTQFSLPNNSQPVFGHTSLTQPVNPNGFT 357
>SPAC1A6.01c ||SPAC23C4.20c|human thyroid receptor interacting
protein homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 455
Score = 24.6 bits (51), Expect = 9.0
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -2
Query: 358 ASITDNLERPVLHVGLHSS 302
A + NL RP+ H LHSS
Sbjct: 408 APVIRNLPRPIYHQDLHSS 426
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 24.6 bits (51), Expect = 9.0
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +3
Query: 84 GIDLGTTYSCVGVFQHGKVEIIANDQGNR 170
G LG TY+ V Q G VE+ D N+
Sbjct: 1279 GCFLGNTYAFASVTQDGSVEVHRLDVNNQ 1307
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 24.6 bits (51), Expect = 9.0
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +3
Query: 189 AFTDTERLIGDAAKNQVAMNPNTQYSMPNVSSDV 290
AF D + G AK A P+T+ + N+S V
Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188
>SPAC2F3.04c |rim1||mitochondrial single-stranded DNA binding
protein Rim1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 24.6 bits (51), Expect = 9.0
Identities = 14/50 (28%), Positives = 23/50 (46%)
Frame = +2
Query: 263 FDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPR 412
FD + I RK V+AD ++G + K+ + + D F+PR
Sbjct: 77 FDRLQTILRKGTRVYVEADAVWRSVPAGNEGSQAKMSLRHVSADVLFYPR 126
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,229,056
Number of Sequences: 5004
Number of extensions: 47296
Number of successful extensions: 178
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 210309424
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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