Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0177.Seq
(518 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 132 2e-31
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 130 7e-31
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 127 6e-30
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 100 8e-22
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 67 9e-12
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 2e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.57
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 1.7
SB_9053| Best HMM Match : TIG (HMM E-Value=0) 29 3.0
SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 4.0
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 28 4.0
SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14) 28 5.3
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 132 bits (319), Expect = 2e-31
Identities = 63/68 (92%), Positives = 65/68 (95%), Gaps = 1/68 (1%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 243 MNP-NTQY 263
MNP NT +
Sbjct: 61 MNPENTVF 68
Score = 105 bits (252), Expect = 2e-23
Identities = 47/87 (54%), Positives = 62/87 (71%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P++T+FDAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFP +
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
L K ++ EAYLG V +AV+T AY
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAY 149
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 130 bits (314), Expect = 7e-31
Identities = 59/63 (93%), Positives = 61/63 (96%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 243 MNP 251
MNP
Sbjct: 61 MNP 63
Score = 89.0 bits (211), Expect = 2e-18
Identities = 43/76 (56%), Positives = 54/76 (71%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P +TIFDAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF + V
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 428 CLRK*RKLPEAYLGKT 475
R K+ Y G+T
Sbjct: 123 AGRP--KVKVEYKGET 136
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 127 bits (306), Expect = 6e-30
Identities = 57/63 (90%), Positives = 61/63 (96%)
Frame = +3
Query: 63 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 243 MNP 251
+NP
Sbjct: 61 LNP 63
Score = 109 bits (261), Expect = 2e-24
Identities = 51/87 (58%), Positives = 66/87 (75%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P++TIFDAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF +
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
L K ++ EAYLG+ V +AVIT AY
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAY 149
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 100 bits (239), Expect = 8e-22
Identities = 48/63 (76%), Positives = 55/63 (87%), Gaps = 2/63 (3%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNP-N 254
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
Query: 255 TQY 263
T +
Sbjct: 764 TVF 766
Score = 86.2 bits (204), Expect = 1e-17
Identities = 42/87 (48%), Positives = 58/87 (66%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPRKSVPW 427
P++T+FDAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF +
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
L K ++ EAYLGK V +AV+T AY
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAY 847
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 66.9 bits (156), Expect = 9e-12
Identities = 32/68 (47%), Positives = 46/68 (67%), Gaps = 3/68 (4%)
Frame = +3
Query: 69 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 242
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 243 MNP-NTQY 263
+NP NT Y
Sbjct: 90 INPKNTIY 97
Score = 41.9 bits (94), Expect = 3e-04
Identities = 27/84 (32%), Positives = 41/84 (48%), Gaps = 1/84 (1%)
Frame = +2
Query: 248 PQHTIFDAKRLIGRKFEDATVQADMKHWPFEVVSD-GGKPKIKVAYKGEDKTFFPRKSVP 424
P++TI+DAKR IG+ F + K + F V +D G V KG P +
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 425 WCLRK*RKLPEAYLGKTVXNAVIT 496
+ ++K R + E L TV AV++
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMS 175
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +3
Query: 81 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 236
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 48.0 bits (109), Expect = 5e-06
Identities = 28/87 (32%), Positives = 44/87 (50%), Gaps = 1/87 (1%)
Frame = +2
Query: 251 QHTIFDAKRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPRKSVPW 427
++TI KR IGRKF D VQ ++ H P++VV I+V Y G+ + F P + +
Sbjct: 120 KNTISQFKRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAM 179
Query: 428 CLRK*RKLPEAYLGKTVXNAVITGSAY 508
+ + E L V + VI+ +Y
Sbjct: 180 LFTRLKTTAEIALKTKVTDCVISVPSY 206
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 42.3 bits (95), Expect = 2e-04
Identities = 27/61 (44%), Positives = 34/61 (55%), Gaps = 3/61 (4%)
Frame = +3
Query: 132 GKVEIIANDQGNRTTPSYVAFTDTERLI--GDAAKNQVAMNP-NTQYSMPNVSSDVSSKM 302
G IIAND G+R TP+ VAF+DTE+LI +V N N Q S V + + KM
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLIDKDGLPYYEVESNERNIQVSPKEVINMIYKKM 142
Query: 303 L 305
L
Sbjct: 143 L 143
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.57
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +3
Query: 141 EIIANDQGNRTTPSYVAFT-DTERL 212
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 1.7
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -3
Query: 249 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 151
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_9053| Best HMM Match : TIG (HMM E-Value=0)
Length = 2990
Score = 28.7 bits (61), Expect = 3.0
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +3
Query: 9 HVC*VVLRTSNKTD*KYKMAKAPAVGIDL 95
HVC +V T K K ++K PA GI+L
Sbjct: 1200 HVCNIVSNTETKITCKLDVSKTPAPGIEL 1228
>SB_51671| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 665
Score = 28.3 bits (60), Expect = 4.0
Identities = 20/75 (26%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Frame = +2
Query: 50 LKIQNGKSTRSRN-RSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCR 226
L++ N + ++ + R GY L R C R + +R + H++L R +RC
Sbjct: 225 LRLVNTRDDKTHDKRPGYRKLSRVC-GGRRPNEPYERTARV-HTILSTETETRFCAKRCA 282
Query: 227 QEPGGDEPQHTIFDA 271
G +P++T F+A
Sbjct: 283 GTASGMDPKNTPFNA 297
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 28.3 bits (60), Expect = 4.0
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = +2
Query: 368 IKVAYKGEDKTFFPRKSVPWCLRK*RKLPEAYLGKTVXNAVITGSAY 508
I V GE ++F P + L + + EA+LG+ V AVIT A+
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAH 48
>SB_30622| Best HMM Match : 7tm_1 (HMM E-Value=9.5e-14)
Length = 856
Score = 27.9 bits (59), Expect = 5.3
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = -2
Query: 259 CVLGFIATWFLAASPMRRSVSVNAT*DGVVLLPWSLAMISTFPCWKTPTQEYVVPRSIPT 80
C+L I WF P+R S + VV+ S+ ++ F C P + P +IP+
Sbjct: 107 CLLLTIERWFAVVQPLRYQASFSKR--RVVVSLISVYCVACFLCCTLPFEVSFSPENIPS 164
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,618,802
Number of Sequences: 59808
Number of extensions: 409237
Number of successful extensions: 1147
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1059
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1138
length of database: 16,821,457
effective HSP length: 77
effective length of database: 12,216,241
effective search space used: 1160542895
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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