BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0175.Seq
(538 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6B12.11 |drc1|sld1|DNA replication protein Drc1|Schizosaccha... 29 0.33
SPAC6B12.16 |meu26||conserved fungal protein|Schizosaccharomyces... 28 0.77
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 27 2.3
SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 25 5.4
SPAC323.07c |||MatE family transporter|Schizosaccharomyces pombe... 25 7.2
>SPAC6B12.11 |drc1|sld1|DNA replication protein
Drc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 337
Score = 29.5 bits (63), Expect = 0.33
Identities = 18/67 (26%), Positives = 33/67 (49%)
Frame = +2
Query: 227 LHTKNSTLLPWYTTPNLVHTKTKCSQQNSSRGRDKRET**NVANNSK*IR*QNFNYLTTS 406
L T +S L YT+PNL+ C + S R+ ++ + +N + I Q F ++S
Sbjct: 170 LETPSSYRLQVYTSPNLLRVNAPCRKSLSEMLRELKDIEDDYGSNEEKIL-QEFESFSSS 228
Query: 407 ESNCMIN 427
S +++
Sbjct: 229 SSESLVD 235
>SPAC6B12.16 |meu26||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 344
Score = 28.3 bits (60), Expect = 0.77
Identities = 11/38 (28%), Positives = 23/38 (60%)
Frame = +3
Query: 198 LNSSELHLYTYIQKIVLCCLGTPHQILFTPKQNAASKI 311
+NS+E+ + Y+ +L + P+ + +TPK + SK+
Sbjct: 1 MNSNEIENFIYLNNTLLSEVPEPNSVFYTPKCFSNSKL 38
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 26.6 bits (56), Expect = 2.3
Identities = 14/34 (41%), Positives = 17/34 (50%), Gaps = 2/34 (5%)
Frame = +2
Query: 230 HTKNSTLLPWYTTPNLVHTKTK--CSQQNSSRGR 325
H + T P T ++VH CS Q SSRGR
Sbjct: 485 HLNDFTSFPTETAVSIVHVPLMFPCSDQTSSRGR 518
>SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2386
Score = 25.4 bits (53), Expect = 5.4
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = -1
Query: 409 FTGS*IIKILLSNLF*IIGNISLGFA--FITTTTRILLAAFCFGVNKIWCGVP 257
FT IK N ++ IS+G + F+ +RI+ CF +N I+ G+P
Sbjct: 247 FTAWTWIKPCFFNFVILLKRISIGDSQLFLHLHSRIVQTLCCFSLNFIYHGLP 299
>SPAC323.07c |||MatE family transporter|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 533
Score = 25.0 bits (52), Expect = 7.2
Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 3/53 (5%)
Frame = -1
Query: 355 GNISLG--FAFITTTTRILLAAFCFGVNKIWCGVPRQQST-IFCM*VYKCNSD 206
G IS+G + F T ++ F G+ IWCG+ T I C N+D
Sbjct: 466 GLISIGSSYLFALPVTVFVVVYFNTGLKGIWCGMILSSVTAITCQFTVLFNTD 518
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,177,730
Number of Sequences: 5004
Number of extensions: 42372
Number of successful extensions: 99
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 222442660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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