BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0168.Seq (508 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 133 2e-32 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 62 5e-11 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 59 3e-10 SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 52 4e-08 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 50 2e-07 SPAC12G12.02 ||SPAC630.01c|rRNA processing protein, unnamed|Schi... 33 0.019 SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 32 0.043 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 31 0.075 SPBC1539.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 29 0.30 SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 29 0.30 SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 29 0.40 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 28 0.92 SPAC1639.02c |trk2|SPAC1F5.12|potassium ion transporter Trk2|Sch... 28 0.92 SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pomb... 27 1.2 SPCC777.07 |||alpha-1,2-mannosyltransferase |Schizosaccharomyces... 26 3.7 SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase |Schizosacchar... 25 4.9 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 4.9 SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S... 25 6.5 SPAC3F10.12c |||transcription factor |Schizosaccharomyces pombe|... 25 6.5 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 25 6.5 SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 25 6.5 SPAPB1E7.09 |ogm2|oma2|protein O-mannosyltransferase Ogm2|Schizo... 25 8.6 SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 25 8.6 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 133 bits (321), Expect = 2e-32 Identities = 61/89 (68%), Positives = 75/89 (84%) Frame = +1 Query: 241 LETAQEEKFIVKATNGPRYVVGCRRQLDKNKLKGGTRVALDMTTLTIMRHLPREVDPLVY 420 L+ E+FIVKA++GPRYVVGCR +D++ L G RV+LDMTTLTIMR LPREVDPLVY Sbjct: 60 LKQLDSERFIVKASSGPRYVVGCRNNVDQSHLVQGVRVSLDMTTLTIMRILPREVDPLVY 119 Query: 421 NMSHEDPGDVTYSAIGGLXEQIRQLREVI 507 NMS EDPGD++++ +GGL EQIR+LREVI Sbjct: 120 NMSIEDPGDISFAGVGGLNEQIRELREVI 148 Score = 67.3 bits (157), Expect = 1e-12 Identities = 29/56 (51%), Positives = 45/56 (80%) Frame = +2 Query: 74 REKAFQDYRKKLMEHKEVESRLKEGRDQLKDLTKQYDKSENDLKALQSVGQIVGEV 241 R+ A + Y+ L++H+E +S+LK+ R +DL K+YDK+E+D+K+LQSVGQI+GEV Sbjct: 4 RDTALEKYKSYLLQHREWDSKLKDLRFGNRDLVKKYDKTEDDIKSLQSVGQIIGEV 59 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 62.1 bits (144), Expect = 5e-11 Identities = 33/89 (37%), Positives = 52/89 (58%) Frame = +1 Query: 241 LETAQEEKFIVKATNGPRYVVGCRRQLDKNKLKGGTRVALDMTTLTIMRHLPREVDPLVY 420 ++T + K +VK +YVV +D ++K RVAL + +++ LP +VDPLV Sbjct: 73 IKTMGKNKVLVKVHPEGKYVVDISPDIDIKEIKPNIRVALRNDSYQLIKILPNKVDPLVS 132 Query: 421 NMSHEDPGDVTYSAIGGLXEQIRQLREVI 507 M E D TY +GGL +QI++++EVI Sbjct: 133 LMMVEKIPDSTYEMVGGLEKQIKEIKEVI 161 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 59.3 bits (137), Expect = 3e-10 Identities = 28/88 (31%), Positives = 47/88 (53%) Frame = +1 Query: 244 ETAQEEKFIVKATNGPRYVVGCRRQLDKNKLKGGTRVALDMTTLTIMRHLPREVDPLVYN 423 ++A++ +++ ++VV ++ ++ G RV D I LP ++DP V Sbjct: 108 QSAEKNAYVINLKQIAKFVVSLGERVSPTDIEEGMRVGCDRNKYAIQLPLPPKIDPSVTM 167 Query: 424 MSHEDPGDVTYSAIGGLXEQIRQLREVI 507 M E+ DVTY +GG EQI +LREV+ Sbjct: 168 MQVEEKPDVTYGDVGGCKEQIERLREVV 195 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 52.4 bits (120), Expect = 4e-08 Identities = 29/89 (32%), Positives = 46/89 (51%) Frame = +1 Query: 241 LETAQEEKFIVKATNGPRYVVGCRRQLDKNKLKGGTRVALDMTTLTIMRHLPREVDPLVY 420 LE + IV +T G YVV LD+ LK VAL + ++ LP E D + Sbjct: 62 LEAIDQNTAIVGSTTGSNYVVRILSTLDRELLKPSASVALQRHSNALVDILPPEADSSIS 121 Query: 421 NMSHEDPGDVTYSAIGGLXEQIRQLREVI 507 + ++ DV+Y+ +GGL Q +++RE + Sbjct: 122 MLRPDERPDVSYADVGGLDVQKQEVREAV 150 Score = 25.8 bits (54), Expect = 3.7 Identities = 13/49 (26%), Positives = 29/49 (59%), Gaps = 2/49 (4%) Frame = +2 Query: 98 RKKLMEHKEVESRLKEG--RDQLKDLTKQYDKSENDLKALQSVGQIVGE 238 R K +E + L+EG ++ K L ++ +++ ++K +QSV ++G+ Sbjct: 12 RWKALERQLEMLDLQEGFIKEDCKSLKRELIRAQEEVKRIQSVPLVIGQ 60 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 50.0 bits (114), Expect = 2e-07 Identities = 26/90 (28%), Positives = 48/90 (53%) Frame = +1 Query: 238 SLETAQEEKFIVKATNGPRYVVGCRRQLDKNKLKGGTRVALDMTTLTIMRHLPREVDPLV 417 +LE ++ + +T GP Y V +DK+ L+ G V L ++I+ L + DP++ Sbjct: 118 TLEEIIDDDHAIVSTAGPEYYVSIMSFVDKDMLEPGCSVLLHHKAMSIVGLLLDDTDPMI 177 Query: 418 YNMSHEDPGDVTYSAIGGLXEQIRQLREVI 507 M + +Y+ IGGL QI++++E + Sbjct: 178 NVMKLDKAPTESYADIGGLESQIQEIKEAV 207 >SPAC12G12.02 ||SPAC630.01c|rRNA processing protein, unnamed|Schizosaccharomyces pombe|chr 1|||Manual Length = 183 Score = 33.5 bits (73), Expect = 0.019 Identities = 17/56 (30%), Positives = 32/56 (57%), Gaps = 1/56 (1%) Frame = +2 Query: 50 STSDMEPLREKAFQDYRK-KLMEHKEVESRLKEGRDQLKDLTKQYDKSENDLKALQ 214 S + +E ++K F+ Y+K + E K+ E R+K+ LKD T +K + K+++ Sbjct: 48 SMAQLEHKKQKIFERYKKVRFFERKKAERRIKQLEKSLKDETMDDEKRKQCEKSMR 103 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 32.3 bits (70), Expect = 0.043 Identities = 17/42 (40%), Positives = 25/42 (59%) Frame = +2 Query: 77 EKAFQDYRKKLMEHKEVESRLKEGRDQLKDLTKQYDKSENDL 202 +K +D RKKL E +E ES L+ R++L+ +S NDL Sbjct: 571 DKLVEDVRKKLQEKEEAESSLRSVRERLEIRISLSVQSINDL 612 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 31.5 bits (68), Expect = 0.075 Identities = 12/23 (52%), Positives = 18/23 (78%) Frame = +1 Query: 439 PGDVTYSAIGGLXEQIRQLREVI 507 P VT+S+IGGL QI Q+R+++ Sbjct: 273 PSAVTFSSIGGLQAQIAQIRDIV 295 >SPBC1539.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 386 Score = 29.5 bits (63), Expect = 0.30 Identities = 18/50 (36%), Positives = 26/50 (52%) Frame = +2 Query: 68 PLREKAFQDYRKKLMEHKEVESRLKEGRDQLKDLTKQYDKSENDLKALQS 217 PL YRK L K+V+ L E + LK L K+ K +ND + L++ Sbjct: 184 PLYPNGQPKYRKILENGKKVKYLLDENGEILKRLVKKEKKLKNDNERLEN 233 >SPBC800.10c |||EPS15 repeat family actin cortical patch component |Schizosaccharomyces pombe|chr 2|||Manual Length = 1116 Score = 29.5 bits (63), Expect = 0.30 Identities = 16/65 (24%), Positives = 34/65 (52%) Frame = +2 Query: 47 ASTSDMEPLREKAFQDYRKKLMEHKEVESRLKEGRDQLKDLTKQYDKSENDLKALQSVGQ 226 A+ SD++ + + Y ++L + K++ ++ + Q + + ++Y E L ALQ + Sbjct: 531 ANLSDVKAKVSEIRKAYDEELAKAKQISLDIETNKAQTEQVNREYSILEATLNALQKQNK 590 Query: 227 IVGEV 241 GEV Sbjct: 591 QKGEV 595 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 29.1 bits (62), Expect = 0.40 Identities = 21/62 (33%), Positives = 33/62 (53%), Gaps = 1/62 (1%) Frame = +2 Query: 56 SDMEPLREKAFQDYRKKLMEHKEVESRLKEGRDQLKDLTKQYDKSENDLK-ALQSVGQIV 232 S E +RE+ Q KKL + + ++SRL + R Q++ Y S + LK + S+ Q V Sbjct: 404 SSCETIREEN-QKNIKKLNDAESLKSRLLQSRTQMQTELDSYITSNSQLKDEITSLKQTV 462 Query: 233 GE 238 E Sbjct: 463 SE 464 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 27.9 bits (59), Expect = 0.92 Identities = 14/42 (33%), Positives = 23/42 (54%) Frame = +2 Query: 59 DMEPLREKAFQDYRKKLMEHKEVESRLKEGRDQLKDLTKQYD 184 +++PL +AFQD K L + V S+L + + D K+ D Sbjct: 544 ELKPLLNEAFQDIEKTLQKLMPVASKLNPLEEVMYDDPKEND 585 >SPAC1639.02c |trk2|SPAC1F5.12|potassium ion transporter Trk2|Schizosaccharomyces pombe|chr 1|||Manual Length = 880 Score = 27.9 bits (59), Expect = 0.92 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 3/52 (5%) Frame = -2 Query: 228 ICPTLCRAFRSFSLLSYCLVKSFSWSRP---SLSRDSTSLCSMSFFL*SWKA 82 + PTL R RS S+L + L KSF+ + P + +R T +MS S+ A Sbjct: 410 VTPTLHRNERSMSVLPFQLAKSFTSALPRRLTFNRTHTKASTMSLPYLSYNA 461 >SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 779 Score = 27.5 bits (58), Expect = 1.2 Identities = 11/27 (40%), Positives = 18/27 (66%) Frame = +1 Query: 427 SHEDPGDVTYSAIGGLXEQIRQLREVI 507 + E P D++ S IGGL + I +L E++ Sbjct: 164 NREPPSDISLSDIGGLDDCINELLELV 190 >SPCC777.07 |||alpha-1,2-mannosyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 378 Score = 25.8 bits (54), Expect = 3.7 Identities = 12/34 (35%), Positives = 17/34 (50%) Frame = -3 Query: 251 AVSRLLQLFVQHFVGLSGHFHSCHIVWSNLLVGH 150 A + L V GLSG ++ CH WSN + + Sbjct: 247 AQNNSLDFIVNDHQGLSGDYNLCHF-WSNFEIAN 279 >SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 413 Score = 25.4 bits (53), Expect = 4.9 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +2 Query: 59 DMEPLREKAFQDYRKKLMEHKEV 127 D+ L ++ F+D RK L EH+ V Sbjct: 58 DLNNLSDEQFEDIRKALFEHQVV 80 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.4 bits (53), Expect = 4.9 Identities = 12/43 (27%), Positives = 24/43 (55%) Frame = +2 Query: 113 EHKEVESRLKEGRDQLKDLTKQYDKSENDLKALQSVGQIVGEV 241 E + +R+ + ++Q+KDL+ D DL+ L+S+ V + Sbjct: 1616 ERLSLTTRMLDLQNQVKDLSNIKDSLSEDLRTLRSLEDSVASL 1658 >SPCC1827.04 |||ankyrin repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 3|||Manual Length = 600 Score = 25.0 bits (52), Expect = 6.5 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 2/53 (3%) Frame = +2 Query: 56 SDMEPLREKAFQDYRKKLME-HKEV-ESRLKEGRDQLKDLTKQYDKSENDLKA 208 S ++ + KA ++ RK+ E KE+ +SRL+E + K L K + ++LKA Sbjct: 351 SHVDSISIKAQEEERKRQAEIEKEIRQSRLQEEERKKKKLAKYTEVIISNLKA 403 >SPAC3F10.12c |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 201 Score = 25.0 bits (52), Expect = 6.5 Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 4/50 (8%) Frame = +2 Query: 95 YRKKLMEHKEVESR----LKEGRDQLKDLTKQYDKSENDLKALQSVGQIV 232 Y+ K + HKEVE R + EG +L ++ +K++ + LQ Q + Sbjct: 86 YKAKRLSHKEVERRRREAISEGIKELANIVPGCEKNKGSI--LQRTAQYI 133 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 25.0 bits (52), Expect = 6.5 Identities = 12/28 (42%), Positives = 14/28 (50%) Frame = -2 Query: 354 NSCSSFKLVFVKLPTTANNVARAISSFY 271 N C S ++F L A AR SSFY Sbjct: 1294 NMCQSHHVIFWDLIDVATTFARVDSSFY 1321 >SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1194 Score = 25.0 bits (52), Expect = 6.5 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +2 Query: 98 RKKLMEHKEVESRLKEGRDQLKDLTKQYDKSENDLKA 208 R +L++ + KE D LK +YD+ EN+LKA Sbjct: 396 RNQLLQINRNINSTKENSDYLKT---EYDEMENELKA 429 >SPAPB1E7.09 |ogm2|oma2|protein O-mannosyltransferase Ogm2|Schizosaccharomyces pombe|chr 1|||Manual Length = 739 Score = 24.6 bits (51), Expect = 8.6 Identities = 11/37 (29%), Positives = 21/37 (56%) Frame = +2 Query: 65 EPLREKAFQDYRKKLMEHKEVESRLKEGRDQLKDLTK 175 E L ++ Q ++ +H E+E + +++LKD TK Sbjct: 4 EQLHAQSGQLRQRFPSKHSEIEDEVANEKEELKDATK 40 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 24.6 bits (51), Expect = 8.6 Identities = 11/36 (30%), Positives = 18/36 (50%) Frame = +1 Query: 400 EVDPLVYNMSHEDPGDVTYSAIGGLXEQIRQLREVI 507 E +P+ +V Y IGG Q+ Q+RE++ Sbjct: 205 EGEPINREDEESSLAEVGYDDIGGCRRQMAQIRELV 240 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,874,622 Number of Sequences: 5004 Number of extensions: 34698 Number of successful extensions: 127 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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