Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0160.Seq
(363 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 139 1e-34
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 139 1e-34
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 139 1e-34
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 79 3e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 73 2e-14
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 43 2e-05
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.003
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.003
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 29 0.22
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 29 0.22
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 27 1.2
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 2.7
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 3.6
SPBC1703.13c |||inorganic phosphate transporter |Schizosaccharom... 25 3.6
SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom... 25 3.6
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 3.6
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 25 4.8
SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomy... 24 6.3
SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 24 6.3
SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 24 6.3
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 24 8.3
SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 24 8.3
SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine l... 24 8.3
SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate transa... 24 8.3
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 139 bits (336), Expect = 1e-34
Identities = 63/67 (94%), Positives = 65/67 (97%)
Frame = +2
Query: 53 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 232
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 233 DKLKAER 253
DKLKAER
Sbjct: 61 DKLKAER 67
Score = 70.1 bits (164), Expect = 1e-13
Identities = 31/36 (86%), Positives = 32/36 (88%)
Frame = +1
Query: 256 RGITIDIAXWKXETSKYYVTIIDAPGHRDFIKNMIT 363
RGITIDIA WK ET KY VT+IDAPGHRDFIKNMIT
Sbjct: 69 RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMIT 104
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 139 bits (336), Expect = 1e-34
Identities = 63/67 (94%), Positives = 65/67 (97%)
Frame = +2
Query: 53 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 232
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 233 DKLKAER 253
DKLKAER
Sbjct: 61 DKLKAER 67
Score = 70.1 bits (164), Expect = 1e-13
Identities = 31/36 (86%), Positives = 32/36 (88%)
Frame = +1
Query: 256 RGITIDIAXWKXETSKYYVTIIDAPGHRDFIKNMIT 363
RGITIDIA WK ET KY VT+IDAPGHRDFIKNMIT
Sbjct: 69 RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMIT 104
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 139 bits (336), Expect = 1e-34
Identities = 63/67 (94%), Positives = 65/67 (97%)
Frame = +2
Query: 53 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 232
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 233 DKLKAER 253
DKLKAER
Sbjct: 61 DKLKAER 67
Score = 70.1 bits (164), Expect = 1e-13
Identities = 31/36 (86%), Positives = 32/36 (88%)
Frame = +1
Query: 256 RGITIDIAXWKXETSKYYVTIIDAPGHRDFIKNMIT 363
RGITIDIA WK ET KY VT+IDAPGHRDFIKNMIT
Sbjct: 69 RGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMIT 104
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 78.6 bits (185), Expect = 3e-16
Identities = 34/63 (53%), Positives = 46/63 (73%)
Frame = +2
Query: 65 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 244
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295
Query: 245 AER 253
ER
Sbjct: 296 EER 298
Score = 33.1 bits (72), Expect = 0.014
Identities = 12/35 (34%), Positives = 22/35 (62%)
Frame = +1
Query: 256 RGITIDIAXWKXETSKYYVTIIDAPGHRDFIKNMI 360
+G T+++ ET +++DAPGH+ ++ NMI
Sbjct: 300 KGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMI 334
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 72.5 bits (170), Expect = 2e-14
Identities = 31/63 (49%), Positives = 44/63 (69%)
Frame = +2
Query: 65 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 244
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD +
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Query: 245 AER 253
ER
Sbjct: 235 EER 237
Score = 42.3 bits (95), Expect = 2e-05
Identities = 19/35 (54%), Positives = 23/35 (65%)
Frame = +1
Query: 256 RGITIDIAXWKXETSKYYVTIIDAPGHRDFIKNMI 360
RG+T+D+A E+ K I DAPGHRDFI MI
Sbjct: 239 RGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMI 273
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 42.7 bits (96), Expect = 2e-05
Identities = 19/36 (52%), Positives = 24/36 (66%)
Frame = +1
Query: 256 RGITIDIAXWKXETSKYYVTIIDAPGHRDFIKNMIT 363
RGITI A + ET+ + +D PGH D+IKNMIT
Sbjct: 100 RGITISSAHVEYETANRHYAHVDCPGHADYIKNMIT 135
Score = 35.1 bits (77), Expect = 0.003
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2
Query: 59 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 145
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.003
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +2
Query: 53 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 148
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 23.8 bits (49), Expect = 8.3
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 304 YYVTIIDAPGHRDF 345
+ V +ID+PGH DF
Sbjct: 98 FLVNLIDSPGHVDF 111
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.003
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +2
Query: 53 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 148
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 23.8 bits (49), Expect = 8.3
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 304 YYVTIIDAPGHRDF 345
+ V +ID+PGH DF
Sbjct: 98 FLVNLIDSPGHVDF 111
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.1 bits (62), Expect = 0.22
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +2
Query: 77 NIVVIGHVDSGKSTTTGHLIYKCGGI 154
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
Score = 25.0 bits (52), Expect = 3.6
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +1
Query: 304 YYVTIIDAPGHRDF 345
Y + IID PGH DF
Sbjct: 145 YNINIIDTPGHIDF 158
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 29.1 bits (62), Expect = 0.22
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +1
Query: 256 RGITIDIAXWKXETSKYYVTIIDAPGHRDF 345
RGITI+ A + +ID PGH DF
Sbjct: 77 RGITINSAAISFTWRNQRINLIDTPGHADF 106
Score = 28.7 bits (61), Expect = 0.29
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +2
Query: 77 NIVVIGHVDSGKSTTTGHLIYKCG 148
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 26.6 bits (56), Expect = 1.2
Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = +1
Query: 250 AWRGITIDIAXWKXETSKYYV--TIIDAPGHRDFIKN 354
A + + I+I + E +++ T+ID PG DFI N
Sbjct: 67 AEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 25.4 bits (53), Expect = 2.7
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -2
Query: 239 VCPIPKHI*RILYPFPGPPSRTSRWYVCQYHHICRSSDQWWWT 111
V +PK I I++ F P S S C+Y S D W T
Sbjct: 31 VLNLPKEILIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRT 73
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 25.0 bits (52), Expect = 3.6
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 47 PKMGKEKTHINIVVIGHVDSGKST 118
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1703.13c |||inorganic phosphate transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 311
Score = 25.0 bits (52), Expect = 3.6
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = -2
Query: 194 PGPPSRTSRWYVCQYHHIC 138
P PP +T + Y QY+ +C
Sbjct: 8 PAPPKKTLQLYTPQYYGLC 26
>SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 910
Score = 25.0 bits (52), Expect = 3.6
Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
Frame = -1
Query: 351 LDEISVSRSINDGNIVLASFXLPXSNID-CDTTPRSAFSLSNTQAYLKDPLPISWASFSN 175
++ +S+ +SIN + SF +++ CD S FS NT + K I ASFS
Sbjct: 264 INTLSLVKSINLKDDESFSFISRLTSVHFCDRQIFSKFSAVNTASTSKTSDTIERASFSK 323
Query: 174 FSMVRLS 154
S + L+
Sbjct: 324 PSFIFLT 330
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 25.0 bits (52), Expect = 3.6
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1
Query: 304 YYVTIIDAPGHRDF 345
Y + +ID+PGH DF
Sbjct: 94 YLINLIDSPGHVDF 107
Score = 23.8 bits (49), Expect = 8.3
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +2
Query: 77 NIVVIGHVDSGKSTTTGHLIYKCGGIDKR 163
N ++ HVD GK+T L+ G I +
Sbjct: 21 NFTLLAHVDHGKTTLADSLLASNGIISSK 49
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 24.6 bits (51), Expect = 4.8
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 77 NIVVIGHVDSGKSTTTGHLIYKCGGIDK 160
N VI H+D GKST + ++ G I++
Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINE 87
Score = 23.8 bits (49), Expect = 8.3
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +1
Query: 304 YYVTIIDAPGHRDF 345
Y + +ID PGH DF
Sbjct: 124 YLLNLIDTPGHVDF 137
>SPCC364.02c |bis1||stress response protein Bis1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 384
Score = 24.2 bits (50), Expect = 6.3
Identities = 18/60 (30%), Positives = 27/60 (45%)
Frame = -1
Query: 327 SINDGNIVLASFXLPXSNIDCDTTPRSAFSLSNTQAYLKDPLPISWASFSNFSMVRLSIP 148
S++ + L S+ P S T A ++YL+ PL S S S + +R SIP
Sbjct: 305 SVDSASTALNSYSTPNSVSRKLTNLTPAARRLVARSYLRSPLHGSSPSASRHTALRTSIP 364
>SPAC2G11.11c |prh1||ATP-dependent RNA helicase
Prh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 719
Score = 24.2 bits (50), Expect = 6.3
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +2
Query: 80 IVVIGHVDSGKSTTTGHLIYKC 145
IVV+G SGKST + +C
Sbjct: 115 IVVVGETGSGKSTQIPQFLNEC 136
>SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 647
Score = 24.2 bits (50), Expect = 6.3
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +2
Query: 83 VVIGHVDSGKSTTTGHLIYKCG 148
+V+GH GK+T +Y+ G
Sbjct: 45 IVLGHTGCGKTTQIPQFLYEAG 66
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 23.8 bits (49), Expect = 8.3
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +2
Query: 59 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKF 178
K T +I+ I + DSGK+ T +Y+ G I +F
Sbjct: 1379 KVTTSASIIAILNNDSGKTVTVKGTVYRDGKEVIEVISRF 1418
>SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase
Prp16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1173
Score = 23.8 bits (49), Expect = 8.3
Identities = 8/23 (34%), Positives = 14/23 (60%)
Frame = +2
Query: 80 IVVIGHVDSGKSTTTGHLIYKCG 148
++V+G SGK+T +Y+ G
Sbjct: 511 LIVVGETGSGKTTQLAQFLYEDG 533
>SPBC405.01 |ade1|min4, SPBC4C3.02c|phosphoribosylamine-glycine
ligase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 788
Score = 23.8 bits (49), Expect = 8.3
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = -2
Query: 275 ISIVIPRHAQPLVCPIPKHI*RILYPFPGPPSRTSR 168
+++VIP PLV I H R+ P GP + +R
Sbjct: 71 VNLVIPGPELPLVEGIEGHFRRVGIPCFGPSALAAR 106
>SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate
transaminase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 696
Score = 23.8 bits (49), Expect = 8.3
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +2
Query: 89 IGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 220
I HV G+ L + GG RTIE E E + KG++ +
Sbjct: 297 IAHVRDGE-LHVHRLRREGGGSTTRTIETLEMEIASVMKGNYDH 339
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,348,745
Number of Sequences: 5004
Number of extensions: 23911
Number of successful extensions: 106
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 89
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 112046990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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