Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0159.Seq
(538 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP... 74 5e-14
At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase, put... 73 9e-14
At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fat... 63 1e-10
At1g10890.1 68414.m01251 F-box family protein contains Pfam PF00... 31 0.49
At4g10750.1 68417.m01755 HpcH/HpaI aldolase family protein simil... 29 2.0
At1g49600.1 68414.m05561 RNA-binding protein 47 (RBP47), putativ... 29 2.0
At5g20990.1 68418.m02495 molybdopterin biosynthesis CNX1 protein... 29 2.6
At1g51710.1 68414.m05827 ubiquitin-specific protease 6, putative... 29 2.6
At4g27000.1 68417.m03884 RNA-binding protein 45 (RBP45), putativ... 28 3.4
At3g19130.1 68416.m02429 RNA-binding protein, putative similar t... 28 4.5
At1g70580.2 68414.m08128 glutamate:glyoxylate aminotransferase 2... 28 4.5
At1g70580.1 68414.m08127 glutamate:glyoxylate aminotransferase 2... 28 4.5
At1g31930.2 68414.m03924 extra-large guanine nucleotide binding ... 28 4.5
At1g31930.1 68414.m03923 extra-large guanine nucleotide binding ... 28 4.5
At4g25650.2 68417.m03694 Rieske [2Fe-2S] domain-containing prote... 27 6.0
At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing prote... 27 6.0
At2g20970.1 68415.m02481 hypothetical protein 27 7.9
At1g47500.1 68414.m05272 RNA-binding protein 47 (RBP47), putativ... 27 7.9
At1g47490.2 68414.m05269 RNA-binding protein 47 (RBP47), putativ... 27 7.9
At1g47490.1 68414.m05270 RNA-binding protein 47 (RBP47), putativ... 27 7.9
At1g23310.1 68414.m02915 glutamate:glyoxylate aminotransferase 1... 27 7.9
>At3g06860.1 68416.m00814 fatty acid multifunctional protein (MFP2)
identical to fatty acid multifunctional protein (AtMFP2)
GB:AF123254 [gi:4337027] (Arabidopsis thaliana) (fatty
acid beta-oxidation); contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 725
Score = 74.1 bits (174), Expect = 5e-14
Identities = 32/81 (39%), Positives = 51/81 (62%)
Frame = +1
Query: 10 PEHTILATNTSSISVNAIGSGIKRKDKFGGLHFFNPVPVMRLLEVIKSDHISEDTYQAMM 189
P+H ILA+NTS+I +N IG K +D+ G HFF+P +M LLE+++++H S ++
Sbjct: 418 PQHCILASNTSTIDLNKIGERTKSQDRIVGAHFFSPAHIMPLLEIVRTNHTSAQVIVDLL 477
Query: 190 EWGKSVGKTCITCKDTPGFVV 252
+ GK + KT + + GF V
Sbjct: 478 DVGKKIKKTPVVVGNCTGFAV 498
Score = 27.5 bits (58), Expect = 6.0
Identities = 18/46 (39%), Positives = 23/46 (50%)
Frame = +3
Query: 255 RILAPYSAEALRMYERGDASAEDIDTAMRLGAGYPMGPLELADYTG 392
R+ PY+ A+ + E G A ID A+ G PMGP L D G
Sbjct: 500 RMFFPYTQAAMFLVECG-ADPYLIDRAISK-FGMPMGPFRLCDLVG 543
>At3g15290.1 68416.m01931 3-hydroxybutyryl-CoA dehydrogenase,
putative similar to S(+)-beta-hydroxybutyryl CoA
dehydrogenase (3-hydroxybutyryl-CoA dehydrogenase)
[Paracoccus denitrificans] GI:12003356; contains Pfam
profiles PF02737: 3-hydroxyacyl-CoA dehydrogenase NAD
binding, PF00725: 3-hydroxyacyl-CoA dehydrogenase
C-terminal
Length = 294
Score = 73.3 bits (172), Expect = 9e-14
Identities = 34/83 (40%), Positives = 51/83 (61%)
Frame = +1
Query: 4 LAPEHTILATNTSSISVNAIGSGIKRKDKFGGLHFFNPVPVMRLLEVIKSDHISEDTYQA 183
+A ILA+NTSSIS+ + S +R + G+HF NP P+M+L+E+I+ SE+T+ A
Sbjct: 108 IAKSSAILASNTSSISITRLASATRRPSQVIGMHFMNPPPIMKLVEIIRGADTSEETFLA 167
Query: 184 MMEWGKSVGKTCITCKDTPGFVV 252
+ GKT + +D GFVV
Sbjct: 168 TKVLAERFGKTTVCSQDYAGFVV 190
Score = 69.7 bits (163), Expect = 1e-12
Identities = 39/93 (41%), Positives = 52/93 (55%)
Frame = +3
Query: 255 RILAPYSAEALRMYERGDASAEDIDTAMRLGAGYPMGPLELADYTGLDLKKSILAVMLKE 434
RIL P EA G A+ EDID+ M+ G +PMGPLELAD GLD+ S++ V+ +
Sbjct: 192 RILMPMINEAFHTLYTGVATKEDIDSGMKHGTNHPMGPLELADLIGLDVCLSVMKVLHEG 251
Query: 435 TGNPVFEPIGVLDXLVKRGEXXDRKPGXGIY*Y 533
G+ + P +L V G RK G G+Y Y
Sbjct: 252 LGDSKYAPCPLLVQYVDAGR-LGRKRGVGVYDY 283
>At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fatty
acid multifunctional protein (AIM1) identical to
gi:4337025; contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 721
Score = 62.9 bits (146), Expect = 1e-10
Identities = 30/79 (37%), Positives = 46/79 (58%)
Frame = +1
Query: 16 HTILATNTSSISVNAIGSGIKRKDKFGGLHFFNPVPVMRLLEVIKSDHISEDTYQAMMEW 195
H ILA+NTS+I ++ IG KD+ G HFF+P +M LLE+++S + S +M
Sbjct: 417 HCILASNTSTIDLDVIGEKTNSKDRIVGAHFFSPAHLMPLLEIVRSKNTSAQVILDLMAV 476
Query: 196 GKSVGKTCITCKDTPGFVV 252
GK++ K + + GF V
Sbjct: 477 GKAIKKVPVVVGNCIGFAV 495
Score = 28.7 bits (61), Expect = 2.6
Identities = 25/93 (26%), Positives = 40/93 (43%)
Frame = +3
Query: 255 RILAPYSAEALRMYERGDASAEDIDTAMRLGAGYPMGPLELADYTGLDLKKSILAVMLKE 434
R PYS +A M ID+ + G P+GP +L D G + ++ + K
Sbjct: 497 RTFFPYS-QAAHMLANLGVDLFRIDSVIT-SFGLPLGPFQLGDLAGHGIGLAVGPIYAKV 554
Query: 435 TGNPVFEPIGVLDXLVKRGEXXDRKPGXGIY*Y 533
G+ +F + + L+K G + G G Y Y
Sbjct: 555 YGDRMFRS-PMTELLLKSGR-NGKINGRGYYIY 585
>At1g10890.1 68414.m01251 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 592
Score = 31.1 bits (67), Expect = 0.49
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3
Query: 95 PNLSFLLMPEPIAFTDIDEVLVARIVC 15
P LSF L + +D+DE++VA I+C
Sbjct: 224 PKLSFALSSKMTTMSDLDEIMVAEILC 250
>At4g10750.1 68417.m01755 HpcH/HpaI aldolase family protein similar
to 2,4-dihydroxydec-2-ene-1,10-dioic acid aldolase
[Sphingopyxis macrogoltabida] GI:23330203; contains Pfam
profile PF03328: HpcH/HpaI aldolase family
Length = 358
Score = 29.1 bits (62), Expect = 2.0
Identities = 21/85 (24%), Positives = 36/85 (42%)
Frame = +3
Query: 69 GHQKER*VRRPPFLQSSTSDATPGGYKKRSHFRGHLPGHDGVGQVCGQDLHHL*GHSRIR 248
GH+K R + + T+D GG ++ G HDG G++ G+ H + G +
Sbjct: 262 GHKKVREMMKKAEKSVLTTDPAKGG----AYLSGFAMPHDGAGEIRGRGYHMVAGAVDVG 317
Query: 249 C*RILAPYSAEALRMYERGDASAED 323
R A +M ++ +ED
Sbjct: 318 LFRNAAVEDVRRFKMGLVNESDSED 342
>At1g49600.1 68414.m05561 RNA-binding protein 47 (RBP47), putative
similar to DNA binding protein ACBF GB:U90212 GI:1899187
from [Nicotiana tabacum]
Length = 445
Score = 29.1 bits (62), Expect = 2.0
Identities = 14/26 (53%), Positives = 15/26 (57%), Gaps = 1/26 (3%)
Frame = +2
Query: 434 NREPGFRTNWGSRXTGEKR-GXXGPE 508
N E FR NW S TGEKR GP+
Sbjct: 187 NAEQPFRLNWASFSTGEKRASENGPD 212
>At5g20990.1 68418.m02495 molybdopterin biosynthesis CNX1 protein /
molybdenum cofactor biosynthesis enzyme CNX1 (CNX1)
identical to SP|Q39054 Molybdopterin biosynthesis CNX1
protein (Molybdenum cofactor biosynthesis enzyme CNX1)
{Arabidopsis thaliana}
Length = 670
Score = 28.7 bits (61), Expect = 2.6
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +1
Query: 127 MRLLEVIKSDHISEDTYQAMMEWGKSVGKTCITCKDTPGFVVKGS 261
+RL E IKSD I + ++A+++W + G TPGFV + +
Sbjct: 357 LRLQEPIKSDPIRPEFHRAIIKWKDNDG------SGTPGFVAEST 395
>At1g51710.1 68414.m05827 ubiquitin-specific protease 6, putative
(UBP6) similar to GI:11993465
Length = 482
Score = 28.7 bits (61), Expect = 2.6
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -2
Query: 276 HCTELGSFNNESWSVLTSDASLAHRLAPLHHGL 178
HC E G ++E+ SV + ++H + LH GL
Sbjct: 238 HCQESGEESSETESVYSLKCHISHEVNHLHEGL 270
>At4g27000.1 68417.m03884 RNA-binding protein 45 (RBP45), putative
DNA binding protein ACBF - Nicotiana tabacum,
PID:g1899188
Length = 415
Score = 28.3 bits (60), Expect = 3.4
Identities = 11/23 (47%), Positives = 12/23 (52%)
Frame = +2
Query: 440 EPGFRTNWGSRXTGEKRGXXGPE 508
E FR NW GE+R GPE
Sbjct: 150 EQAFRLNWAQLGAGERRQAEGPE 172
>At3g19130.1 68416.m02429 RNA-binding protein, putative similar to
RNA Binding Protein 47 [Nicotiana plumbaginifolia]
GI:9663769, DNA binding protein ACBF GB:AAC49850 from
[Nicotiana tabacum]; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 435
Score = 27.9 bits (59), Expect = 4.5
Identities = 13/26 (50%), Positives = 15/26 (57%), Gaps = 1/26 (3%)
Frame = +2
Query: 434 NREPGFRTNWGSRXTGEKRG-XXGPE 508
N + FR NW S TGEKR GP+
Sbjct: 176 NSDQPFRINWASFSTGEKRAVENGPD 201
>At1g70580.2 68414.m08128 glutamate:glyoxylate aminotransferase 2
(GGT2) identical to glutamate:glyoxylate
aminotransferase 2 [Arabidopsis thaliana] GI:24461829;
similar to alanine aminotransferase from Panicum
miliaceum [SP|P34106], GI:4730884 from Oryza sativa;
contains Pfam profile PF00155: aminotransferase, classes
I and II
Length = 481
Score = 27.9 bits (59), Expect = 4.5
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 195 PLHHGLVSVLGNVIAFYNLQESHHW 121
PL+ +S+LG + Y L+ES +W
Sbjct: 166 PLYSATISLLGGTLVPYYLEESENW 190
>At1g70580.1 68414.m08127 glutamate:glyoxylate aminotransferase 2
(GGT2) identical to glutamate:glyoxylate
aminotransferase 2 [Arabidopsis thaliana] GI:24461829;
similar to alanine aminotransferase from Panicum
miliaceum [SP|P34106], GI:4730884 from Oryza sativa;
contains Pfam profile PF00155: aminotransferase, classes
I and II
Length = 481
Score = 27.9 bits (59), Expect = 4.5
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 195 PLHHGLVSVLGNVIAFYNLQESHHW 121
PL+ +S+LG + Y L+ES +W
Sbjct: 166 PLYSATISLLGGTLVPYYLEESENW 190
>At1g31930.2 68414.m03924 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 848
Score = 27.9 bits (59), Expect = 4.5
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +3
Query: 234 HSRIRC*RILAPYSAEALRMYERGDASAEDIDTAMRLGAGYPMGPLELAD 383
HSR+ R+L+P + + E+ + +A + + GYP+ P E+AD
Sbjct: 234 HSRVLS-RLLSPLEVKQIMKAEK-ECTANQLRPEQLIVNGYPLKPEEMAD 281
>At1g31930.1 68414.m03923 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 848
Score = 27.9 bits (59), Expect = 4.5
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +3
Query: 234 HSRIRC*RILAPYSAEALRMYERGDASAEDIDTAMRLGAGYPMGPLELAD 383
HSR+ R+L+P + + E+ + +A + + GYP+ P E+AD
Sbjct: 234 HSRVLS-RLLSPLEVKQIMKAEK-ECTANQLRPEQLIVNGYPLKPEEMAD 281
>At4g25650.2 68417.m03694 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 559
Score = 27.5 bits (58), Expect = 6.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 252 NNESWSVLTSDASLAHRLAPLHHGLVSVLGNVIAFYN 142
N + W V+ D + HRLAPL G + G + Y+
Sbjct: 116 NEKQWKVM--DDTCPHRLAPLSDGRIDQWGRLQCVYH 150
>At4g25650.1 68417.m03693 Rieske [2Fe-2S] domain-containing protein
similar to cell death suppressor protein lls1 from Zea
mays [gi:1935909], Rieske iron-sulfur protein Tic55 from
Pisum sativum [gi:2764524]; contains Pfam PF00355 Rieske
[2Fe-2S] domain
Length = 536
Score = 27.5 bits (58), Expect = 6.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 252 NNESWSVLTSDASLAHRLAPLHHGLVSVLGNVIAFYN 142
N + W V+ D + HRLAPL G + G + Y+
Sbjct: 116 NEKQWKVM--DDTCPHRLAPLSDGRIDQWGRLQCVYH 150
>At2g20970.1 68415.m02481 hypothetical protein
Length = 373
Score = 27.1 bits (57), Expect = 7.9
Identities = 17/43 (39%), Positives = 20/43 (46%)
Frame = +3
Query: 36 HFINISERYRLGHQKER*VRRPPFLQSSTSDATPGGYKKRSHF 164
H I + E + HQ V PP S SD TPG Y RS +
Sbjct: 113 HSIYMFEHFLRSHQ----VVPPPPPPQSKSDETPGNYINRSSY 151
>At1g47500.1 68414.m05272 RNA-binding protein 47 (RBP47), putative
similar to DNA binding protein GI:1899187 from
[Nicotiana tabacum]
Length = 434
Score = 27.1 bits (57), Expect = 7.9
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +2
Query: 434 NREPGFRTNWGSRXTGEKR 490
N + FR NW S TGEKR
Sbjct: 173 NTDQPFRLNWASFSTGEKR 191
>At1g47490.2 68414.m05269 RNA-binding protein 47 (RBP47), putative
similar to DNA binding protein GI:1899187 from
[Nicotiana tabacum]
Length = 310
Score = 27.1 bits (57), Expect = 7.9
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +2
Query: 434 NREPGFRTNWGSRXTGEKR 490
N + FR NW S TGEKR
Sbjct: 171 NTDQPFRLNWASFSTGEKR 189
>At1g47490.1 68414.m05270 RNA-binding protein 47 (RBP47), putative
similar to DNA binding protein GI:1899187 from
[Nicotiana tabacum]
Length = 432
Score = 27.1 bits (57), Expect = 7.9
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +2
Query: 434 NREPGFRTNWGSRXTGEKR 490
N + FR NW S TGEKR
Sbjct: 171 NTDQPFRLNWASFSTGEKR 189
>At1g23310.1 68414.m02915 glutamate:glyoxylate aminotransferase 1
(GGT1) identical to glutamate:glyoxylate
aminotransferase 1 [Arabidopsis thaliana] GI:24461827;
similar to alanine aminotransferase GI:4730884 from
[Oryza sativa]; contains Pfam profile PF00155:
aminotransferase, classes I and II
Length = 481
Score = 27.1 bits (57), Expect = 7.9
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -2
Query: 195 PLHHGLVSVLGNVIAFYNLQESHHW 121
PL+ +S+LG + Y L ES +W
Sbjct: 166 PLYSATISLLGGTLVPYYLDESENW 190
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,843,475
Number of Sequences: 28952
Number of extensions: 248751
Number of successful extensions: 589
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 575
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 589
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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