Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0158.Seq
(458 letters)
Database: rice
37,544 sequences; 14,793,348 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
07_01_0762 - 5852168-5852266,5852409-5852569,5852691-5852823 122 1e-28
02_03_0219 + 16541350-16541482,16541605-16541765,16541863-165419... 122 2e-28
02_02_0303 - 8766264-8766362,8767112-8767272,8768160-8768289 75 3e-14
01_01_0365 - 2859617-2859722,2860047-2860489,2862232-2862391,286... 33 0.15
10_08_0314 + 16683473-16683564,16683945-16683945,16684057-166842... 27 7.3
09_04_0211 - 15656614-15657186 27 9.6
05_04_0367 - 20672776-20673550,20674097-20674320,20674626-206748... 27 9.6
>07_01_0762 - 5852168-5852266,5852409-5852569,5852691-5852823
Length = 130
Score = 122 bits (295), Expect = 1e-28
Identities = 57/71 (80%), Positives = 65/71 (91%)
Frame = +3
Query: 42 MVRMNVLSDALKSIHNAEKRGKRQVLIRPCSKVIVKFLTVMMKHGYIGEFEIVDDHRAGK 221
MVR++VL+DALK+++NAEKRGKRQVLIRP SKVI+KFL VM KHGYIGEFE VDDHR+GK
Sbjct: 1 MVRVSVLNDALKTMYNAEKRGKRQVLIRPSSKVIIKFLIVMQKHGYIGEFEFVDDHRSGK 60
Query: 222 IVVNLTGRLNK 254
IVV L GRLNK
Sbjct: 61 IVVELNGRLNK 71
Score = 45.6 bits (103), Expect = 2e-05
Identities = 21/28 (75%), Positives = 23/28 (82%), Gaps = 1/28 (3%)
Frame = +2
Query: 257 GVISPRFDVPINDIERWT-NLLPSRQFG 337
GVISPRFDV + +IE WT LLPSRQFG
Sbjct: 73 GVISPRFDVGVKEIESWTARLLPSRQFG 100
Score = 36.3 bits (80), Expect = 0.012
Identities = 15/20 (75%), Positives = 18/20 (90%)
Frame = +1
Query: 337 FLVLTTSGGIMDHEEARXKH 396
++VLTTS GIMDHEEAR K+
Sbjct: 101 YIVLTTSAGIMDHEEARRKN 120
>02_03_0219 +
16541350-16541482,16541605-16541765,16541863-16541940,
16543176-16543445
Length = 213
Score = 122 bits (293), Expect = 2e-28
Identities = 56/71 (78%), Positives = 65/71 (91%)
Frame = +3
Query: 42 MVRMNVLSDALKSIHNAEKRGKRQVLIRPCSKVIVKFLTVMMKHGYIGEFEIVDDHRAGK 221
MVR++VL+DALK+++NAEKRGKRQV+IRP SKVI+KFL VM KHGYIGEFE VDDHR+GK
Sbjct: 1 MVRVSVLNDALKTMYNAEKRGKRQVMIRPSSKVIIKFLIVMQKHGYIGEFEFVDDHRSGK 60
Query: 222 IVVNLTGRLNK 254
IVV L GRLNK
Sbjct: 61 IVVELNGRLNK 71
Score = 45.6 bits (103), Expect = 2e-05
Identities = 21/28 (75%), Positives = 23/28 (82%), Gaps = 1/28 (3%)
Frame = +2
Query: 257 GVISPRFDVPINDIERWT-NLLPSRQFG 337
GVISPRFDV + +IE WT LLPSRQFG
Sbjct: 73 GVISPRFDVGVKEIESWTARLLPSRQFG 100
Score = 36.3 bits (80), Expect = 0.012
Identities = 15/20 (75%), Positives = 18/20 (90%)
Frame = +1
Query: 337 FLVLTTSGGIMDHEEARXKH 396
++VLTTS GIMDHEEAR K+
Sbjct: 101 YIVLTTSAGIMDHEEARRKN 120
>02_02_0303 - 8766264-8766362,8767112-8767272,8768160-8768289
Length = 129
Score = 74.9 bits (176), Expect = 3e-14
Identities = 33/64 (51%), Positives = 47/64 (73%)
Frame = +3
Query: 57 VLSDALKSIHNAEKRGKRQVLIRPCSKVIVKFLTVMMKHGYIGEFEIVDDHRAGKIVVNL 236
+L+DAL+++ NAE+RGK L++P S V+V FL +M GYI +FE++D HR GKI V L
Sbjct: 5 ILNDALRTMVNAERRGKATALLQPISGVMVSFLNIMKHRGYIKKFEVIDPHRVGKINVEL 64
Query: 237 TGRL 248
GR+
Sbjct: 65 HGRI 68
Score = 28.3 bits (60), Expect = 3.1
Identities = 11/21 (52%), Positives = 16/21 (76%), Gaps = 1/21 (4%)
Frame = +1
Query: 325 TTVW-FLVLTTSGGIMDHEEA 384
T W ++V+TT G++DHEEA
Sbjct: 95 TRQWGYVVITTPNGVLDHEEA 115
>01_01_0365 -
2859617-2859722,2860047-2860489,2862232-2862391,
2863431-2863516,2863648-2866272
Length = 1139
Score = 32.7 bits (71), Expect = 0.15
Identities = 14/25 (56%), Positives = 20/25 (80%), Gaps = 2/25 (8%)
Frame = +3
Query: 198 VDDHRAGKIVVNLTGRLNK--VVSF 266
VDDH++G+I++ GRLNK V+SF
Sbjct: 912 VDDHKSGEIILEFDGRLNKWGVISF 936
>10_08_0314 +
16683473-16683564,16683945-16683945,16684057-16684268,
16684655-16684747,16685106-16685160,16685480-16685566
Length = 179
Score = 27.1 bits (57), Expect = 7.3
Identities = 14/58 (24%), Positives = 31/58 (53%)
Frame = +3
Query: 132 SKVIVKFLTVMMKHGYIGEFEIVDDHRAGKIVVNLTGRLNKVVSFHLVLMFPSTILKD 305
S +++K L V++ +G + ++ RA + + + G +FHL+ FP+ ++ D
Sbjct: 36 SVIVLKCLFVLLHDKKMGIYALIYVLRACRYIEDDEGIRKYFAAFHLLGSFPAAVIID 93
>09_04_0211 - 15656614-15657186
Length = 190
Score = 26.6 bits (56), Expect = 9.6
Identities = 8/20 (40%), Positives = 14/20 (70%)
Frame = +2
Query: 206 SQSWQDCCKSHRQTKQGGVI 265
+ SW C +SHR +++G +I
Sbjct: 72 TSSWPRCLRSHRSSREGALI 91
>05_04_0367 -
20672776-20673550,20674097-20674320,20674626-20674843,
20675133-20676534
Length = 872
Score = 26.6 bits (56), Expect = 9.6
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -3
Query: 426 KKKA*NFSSKVFXSGFFMVHDATTCCKD*EPNCREGSRLVHLSISLMGTSKRGEMTPP 253
KK++ + S+ SG +H T D EPN + + + G RGE++PP
Sbjct: 84 KKRSASSSTLHTDSGHSHLHFHTDGGSDSEPNSADDDCACGAAAAAAGHGVRGEISPP 141
Database: rice
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 14,793,348
Number of sequences in database: 37,544
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,316,450
Number of Sequences: 37544
Number of extensions: 188343
Number of successful extensions: 383
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 376
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 381
length of database: 14,793,348
effective HSP length: 76
effective length of database: 11,940,004
effective search space used: 907440304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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