BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0156.Seq
(399 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1783.08c |rpl1502|rpl15-2|60S ribosomal protein L15b|Schizos... 75 5e-15
SPCC576.11 |rpl15||60S ribosomal protein L15|Schizosaccharomyces... 73 2e-14
SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 4.3
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 25 4.3
SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 25 4.3
SPAC23H3.15c ||SPAC25H1.01c|sequence orphan|Schizosaccharomyces ... 25 4.3
SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein L25|Schizosacch... 25 5.7
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc... 25 5.7
SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po... 24 7.6
SPCC1235.08c |pdh1||DUF1751 family protein|Schizosaccharomyces p... 24 10.0
SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 24 10.0
>SPAC1783.08c |rpl1502|rpl15-2|60S ribosomal protein
L15b|Schizosaccharomyces pombe|chr 1|||Manual
Length = 201
Score = 74.5 bits (175), Expect = 5e-15
Identities = 35/54 (64%), Positives = 40/54 (74%)
Frame = -1
Query: 252 IVNAVHKHREMRGLTSAGRSSRGLGKGHRYSQTKGGSRRAAWLRRNTLQLRRKR 91
IVN VHKHRE RGLTS G+ SRG+GKGHRY+ + + A WLR NTL LRR R
Sbjct: 151 IVNPVHKHRESRGLTSIGKKSRGIGKGHRYNNS---PQHATWLRHNTLSLRRYR 201
Score = 63.7 bits (148), Expect = 1e-11
Identities = 26/31 (83%), Positives = 30/31 (96%)
Frame = -2
Query: 347 YWVAQDSSYKYFEVILVDPSHKAIRRDPKIN 255
YWV QD++YK+FEVILVDPSHKAIRRDP+IN
Sbjct: 119 YWVNQDATYKFFEVILVDPSHKAIRRDPRIN 149
>SPCC576.11 |rpl15||60S ribosomal protein L15|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 201
Score = 72.9 bits (171), Expect = 2e-14
Identities = 34/54 (62%), Positives = 40/54 (74%)
Frame = -1
Query: 252 IVNAVHKHREMRGLTSAGRSSRGLGKGHRYSQTKGGSRRAAWLRRNTLQLRRKR 91
IVN VHKHRE RGLTS G+ SRG+GKGHR++ + + A WLR NTL LRR R
Sbjct: 151 IVNPVHKHRESRGLTSIGKKSRGIGKGHRFNNS---PQHATWLRHNTLSLRRYR 201
Score = 63.7 bits (148), Expect = 1e-11
Identities = 26/31 (83%), Positives = 30/31 (96%)
Frame = -2
Query: 347 YWVAQDSSYKYFEVILVDPSHKAIRRDPKIN 255
YWV QD++YK+FEVILVDPSHKAIRRDP+IN
Sbjct: 119 YWVNQDATYKFFEVILVDPSHKAIRRDPRIN 149
>SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 865
Score = 25.0 bits (52), Expect = 4.3
Identities = 6/19 (31%), Positives = 13/19 (68%)
Frame = -3
Query: 58 HLLIFVCRWKASGLIKYYC 2
++++F+C W G ++ YC
Sbjct: 649 NIILFLCLWVQGGRVRAYC 667
>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2609
Score = 25.0 bits (52), Expect = 4.3
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -1
Query: 378 SLRWSPCVELLLGCTRFF 325
SL+ P V LL+G TRFF
Sbjct: 568 SLKVPPLVPLLIGTTRFF 585
>SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex
subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1522
Score = 25.0 bits (52), Expect = 4.3
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = -1
Query: 390 ACXPSLRWSPCVELLLGCT 334
+C PS R P +ELLL C+
Sbjct: 595 SCGPSYRLLPKIELLLPCS 613
>SPAC23H3.15c ||SPAC25H1.01c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 325
Score = 25.0 bits (52), Expect = 4.3
Identities = 15/34 (44%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = -1
Query: 213 LTSAGRSSRGLGKG-HRYSQTKGGSRRAAWLRRN 115
++SAG S G GKG + T +RRAA RN
Sbjct: 236 VSSAGYSGEGYGKGTYATDTTAEANRRAATGTRN 269
>SPBC106.18 |rpl2501|rpl25a|60S ribosomal protein
L25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 141
Score = 24.6 bits (51), Expect = 5.7
Identities = 8/24 (33%), Positives = 16/24 (66%)
Frame = +3
Query: 18 NPLAFHLHTKISKWKVTDSFR*MF 89
N L FH+H K +K+ + ++ R ++
Sbjct: 79 NTLVFHVHLKANKFTIKEAVRKLY 102
>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 24.6 bits (51), Expect = 5.7
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = -2
Query: 146 AHAGQLGSDATLFNYVAN 93
A AG + DAT F YV N
Sbjct: 240 ARAGMIAPDATTFEYVKN 257
>SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 833
Score = 24.2 bits (50), Expect = 7.6
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = -2
Query: 158 KQREAHAGQLGSDATLFNYVANDKHLSKTVSD 63
KQ E +G + F+ ND+HL + +D
Sbjct: 263 KQPEEEVSVVGDELKSFDKENNDEHLERVTND 294
>SPCC1235.08c |pdh1||DUF1751 family protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 226
Score = 23.8 bits (49), Expect = 10.0
Identities = 7/26 (26%), Positives = 14/26 (53%)
Frame = -2
Query: 86 HLSKTVSDFPFAYFCMQMESQWVNKI 9
HL FP+ YFC+ + ++++
Sbjct: 178 HLFYVFQSFPWTYFCLAVSGTCISEL 203
>SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 2386
Score = 23.8 bits (49), Expect = 10.0
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = -3
Query: 298 WTRHTRPFVAILRSTDRE 245
W HTRPF IL + R+
Sbjct: 2135 WVNHTRPFREILLKSYRQ 2152
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,518,443
Number of Sequences: 5004
Number of extensions: 25781
Number of successful extensions: 66
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 61
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 64
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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