Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0156.Seq
(399 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g16720.1 68417.m02526 60S ribosomal protein L15 (RPL15A) 64 2e-11
At4g17390.1 68417.m02606 60S ribosomal protein L15 (RPL15B) 64 4e-11
At4g18440.1 68417.m02736 adenylosuccinate lyase, putative / aden... 27 3.5
At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative s... 27 6.1
At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloropl... 27 6.1
At1g75560.1 68414.m08781 zinc knuckle (CCHC-type) family protein... 27 6.1
At5g16300.2 68418.m01906 expressed protein 26 8.0
At5g16300.1 68418.m01905 expressed protein 26 8.0
>At4g16720.1 68417.m02526 60S ribosomal protein L15 (RPL15A)
Length = 204
Score = 64.5 bits (150), Expect = 2e-11
Identities = 32/55 (58%), Positives = 39/55 (70%), Gaps = 1/55 (1%)
Frame = -1
Query: 252 IVNAVHKHREMRGLTSAGRSSRGL-GKGHRYSQTKGGSRRAAWLRRNTLQLRRKR 91
I N VHKHRE+RGLTS G+ +RGL GKGH + + SRRA W + N+L LRR R
Sbjct: 151 ICNPVHKHRELRGLTSEGKKNRGLRGKGHNNHKNR-PSRRATWKKNNSLSLRRYR 204
Score = 56.8 bits (131), Expect = 5e-09
Identities = 20/31 (64%), Positives = 28/31 (90%)
Frame = -2
Query: 347 YWVAQDSSYKYFEVILVDPSHKAIRRDPKIN 255
YW+ +DS+YKY+E+ILVDP+H A+R DP+IN
Sbjct: 119 YWLNEDSTYKYYEIILVDPAHNAVRNDPRIN 149
>At4g17390.1 68417.m02606 60S ribosomal protein L15 (RPL15B)
Length = 204
Score = 63.7 bits (148), Expect = 4e-11
Identities = 31/55 (56%), Positives = 39/55 (70%), Gaps = 1/55 (1%)
Frame = -1
Query: 252 IVNAVHKHREMRGLTSAGRSSRGL-GKGHRYSQTKGGSRRAAWLRRNTLQLRRKR 91
I N VHKHRE+RGLTS G+ +RGL GKGH + + SRRA W + N++ LRR R
Sbjct: 151 ICNPVHKHRELRGLTSEGKKNRGLRGKGHNNHKNR-PSRRATWKKNNSISLRRYR 204
Score = 56.8 bits (131), Expect = 5e-09
Identities = 20/31 (64%), Positives = 28/31 (90%)
Frame = -2
Query: 347 YWVAQDSSYKYFEVILVDPSHKAIRRDPKIN 255
YW+ +DS+YKY+E+ILVDP+H A+R DP+IN
Sbjct: 119 YWLNEDSTYKYYEIILVDPAHNAVRNDPRIN 149
>At4g18440.1 68417.m02736 adenylosuccinate lyase, putative /
adenylosuccinase, putative similar to SP|P25739
Adenylosuccinate lyase (EC 4.3.2.2) (Adenylosuccinase)
{Escherichia coli}; contains Pfam profile PF00206: Lyase
Length = 536
Score = 27.5 bits (58), Expect = 3.5
Identities = 18/67 (26%), Positives = 37/67 (55%), Gaps = 1/67 (1%)
Frame = -2
Query: 206 RLVAAPEVSARDIDTLKQREAHAGQLGSDATLFN-YVANDKHLSKTVSDFPFAYFCMQME 30
+LV + +V+A +D + R+ L + + L Y + K L+ ++S+F YF + +E
Sbjct: 56 KLVTSTKVTA--MDGVSSRDLEMSNLTALSPLDGRYWSKVKDLASSLSEFGLIYFRVFVE 113
Query: 29 SQWVNKI 9
+W+ K+
Sbjct: 114 IKWLLKL 120
>At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative
similar to Swiss-Prot:Q9NG98 DNA topoisomerase III alpha
[Drosophila melanogaster]
Length = 926
Score = 26.6 bits (56), Expect = 6.1
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -1
Query: 207 SAGRSSRGLGKGHRYSQTKGGSR 139
S R SRG G+G R Q+ GG R
Sbjct: 861 SGRRGSRGRGRGGRGGQSSGGRR 883
>At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloroplast
(FAD7) (FADD) identical to omega-3 fatty acid
desaturase, chloroplast precursor SP:P46310 [Arabidopsis
thaliana (Mouse-ear cress)]; identical to Pfam profile
PF00487: Fatty acid desaturase; identical to cDNA
plastid fatty acid desaturase GI:809491
Length = 446
Score = 26.6 bits (56), Expect = 6.1
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -2
Query: 347 YWVAQDSSYKYFEVILVDPSHKAIRRDPKIN 255
YW+AQ + + V+ D H + DPK+N
Sbjct: 147 YWLAQGTMFWALFVLGHDCGHGSFSNDPKLN 177
>At1g75560.1 68414.m08781 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 257
Score = 26.6 bits (56), Expect = 6.1
Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +3
Query: 177 CRDLGSCDQ-PKSDHASRDAYVQHSRSVDLRIATN 278
C + G C KS H +RD SR+ DLR+ N
Sbjct: 108 CSNEGICHSCGKSGHRARDCSNSDSRAGDLRLCNN 142
>At5g16300.2 68418.m01906 expressed protein
Length = 1034
Score = 26.2 bits (55), Expect = 8.0
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 226 AMLMYSIHDPLILGSRRMALCDGSTRITSK 315
A+L +S H PLILGS R+ + T ++ K
Sbjct: 617 ALLNHSKHVPLILGSPRLWCRETMTAVSDK 646
>At5g16300.1 68418.m01905 expressed protein
Length = 1068
Score = 26.2 bits (55), Expect = 8.0
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 226 AMLMYSIHDPLILGSRRMALCDGSTRITSK 315
A+L +S H PLILGS R+ + T ++ K
Sbjct: 617 ALLNHSKHVPLILGSPRLWCRETMTAVSDK 646
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,070,233
Number of Sequences: 28952
Number of extensions: 140644
Number of successful extensions: 398
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 391
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 398
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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