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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0153.Seq
         (399 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    26   2.5  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    25   3.3  
SPCC417.04 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual         25   4.3  
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    25   4.3  
SPCC417.09c |||transcription factor |Schizosaccharomyces pombe|c...    25   5.7  
SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyce...    24   10.0 
SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces po...    24   10.0 
SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb...    24   10.0 

>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 25.8 bits (54), Expect = 2.5
 Identities = 13/35 (37%), Positives = 16/35 (45%)
 Frame = +2

Query: 5    ALMDTVPTVVMEVYHPPAVPVTLMQAQTLQDPVHP 109
            A+    P  V E YHPP    T  +   +Q P HP
Sbjct: 922  AIASPPPPKVGETYHPPTASGT--RVPPVQQPSHP 954



 Score = 25.0 bits (52), Expect = 4.3
 Identities = 20/58 (34%), Positives = 23/58 (39%)
 Frame = +2

Query: 50   PPAVPVTLMQAQTLQDPVHPAVAALMDTVPTVVMEVYHPPAVPVTLMQAQTWQDPVHP 223
            PP  P   M A        PA+A+     P  V E YHPP    T  +    Q P HP
Sbjct: 908  PPPPPTASMTASA------PAIAS---PPPPKVGETYHPPTASGT--RVPPVQQPSHP 954


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 25.4 bits (53), Expect = 3.3
 Identities = 14/30 (46%), Positives = 17/30 (56%)
 Frame = +2

Query: 77   QAQTLQDPVHPAVAALMDTVPTVVMEVYHP 166
            QA  +   V PAV+A + T P VV  V HP
Sbjct: 1284 QASAVSSNV-PAVSASISTPPAVVPTVQHP 1312


>SPCC417.04 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 180

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 12/28 (42%), Positives = 15/28 (53%)
 Frame = -1

Query: 291 WNCRXMIHFHHHIGTVSISAATAGCTGS 208
           W     IH  + I TV I ++T  CTGS
Sbjct: 124 WCNSETIHLTYLIDTVVIESSTFWCTGS 151


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
           Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 25.0 bits (52), Expect = 4.3
 Identities = 11/35 (31%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
 Frame = -3

Query: 313 SVLASVLLEL-PVXDTLPSPHRNCIHKCCYRRMYW 212
           S+L   LL L    ++L S   +C+  CC    +W
Sbjct: 248 SILKDYLLNLVKTSESLSSQQMSCLRICCNSSSFW 282


>SPCC417.09c |||transcription factor |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 767

 Score = 24.6 bits (51), Expect = 5.7
 Identities = 10/21 (47%), Positives = 15/21 (71%)
 Frame = -3

Query: 334 DVLDPARSVLASVLLELPVXD 272
           D+LDPA + +AS++  LP  D
Sbjct: 264 DILDPADNTVASLIHALPPKD 284


>SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 363

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 8/16 (50%), Positives = 10/16 (62%)
 Frame = -1

Query: 240 ISAATAGCTGSCQVCA 193
           +S  T GCT  C+ CA
Sbjct: 62  LSIKTGGCTEDCKYCA 77



 Score = 23.8 bits (49), Expect = 10.0
 Identities = 8/12 (66%), Positives = 8/12 (66%)
 Frame = -2

Query: 341 TAGCTGSCKVCA 306
           T GCT  CK CA
Sbjct: 66  TGGCTEDCKYCA 77



 Score = 23.8 bits (49), Expect = 10.0
 Identities = 8/12 (66%), Positives = 8/12 (66%)
 Frame = -1

Query: 114 TAGCTGSCKVCA 79
           T GCT  CK CA
Sbjct: 66  TGGCTEDCKYCA 77


>SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 516

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = +2

Query: 104 HPAVAALMDTVPTVVMEVYHPPAVPV 181
           HPAV+A   T P+  +EVY    + V
Sbjct: 254 HPAVSAFYVTCPSKGIEVYGQDQIAV 279


>SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 372

 Score = 23.8 bits (49), Expect = 10.0
 Identities = 7/24 (29%), Positives = 13/24 (54%), Gaps = 2/24 (8%)
 Frame = -3

Query: 196 CLHQCYWNC--RWMIHFHHDCRNC 131
           C H+ +  C  +W++ + H C  C
Sbjct: 339 CKHEFHRPCIAKWIVDYRHACPTC 362


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,462,686
Number of Sequences: 5004
Number of extensions: 27506
Number of successful extensions: 76
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 60
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 76
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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