BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0153.Seq
(399 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 2.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 25 3.3
SPCC417.04 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 25 4.3
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 25 4.3
SPCC417.09c |||transcription factor |Schizosaccharomyces pombe|c... 25 5.7
SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyce... 24 10.0
SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces po... 24 10.0
SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb... 24 10.0
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.8 bits (54), Expect = 2.5
Identities = 13/35 (37%), Positives = 16/35 (45%)
Frame = +2
Query: 5 ALMDTVPTVVMEVYHPPAVPVTLMQAQTLQDPVHP 109
A+ P V E YHPP T + +Q P HP
Sbjct: 922 AIASPPPPKVGETYHPPTASGT--RVPPVQQPSHP 954
Score = 25.0 bits (52), Expect = 4.3
Identities = 20/58 (34%), Positives = 23/58 (39%)
Frame = +2
Query: 50 PPAVPVTLMQAQTLQDPVHPAVAALMDTVPTVVMEVYHPPAVPVTLMQAQTWQDPVHP 223
PP P M A PA+A+ P V E YHPP T + Q P HP
Sbjct: 908 PPPPPTASMTASA------PAIAS---PPPPKVGETYHPPTASGT--RVPPVQQPSHP 954
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.4 bits (53), Expect = 3.3
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +2
Query: 77 QAQTLQDPVHPAVAALMDTVPTVVMEVYHP 166
QA + V PAV+A + T P VV V HP
Sbjct: 1284 QASAVSSNV-PAVSASISTPPAVVPTVQHP 1312
>SPCC417.04 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
Length = 180
Score = 25.0 bits (52), Expect = 4.3
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -1
Query: 291 WNCRXMIHFHHHIGTVSISAATAGCTGS 208
W IH + I TV I ++T CTGS
Sbjct: 124 WCNSETIHLTYLIDTVVIESSTFWCTGS 151
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 25.0 bits (52), Expect = 4.3
Identities = 11/35 (31%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = -3
Query: 313 SVLASVLLEL-PVXDTLPSPHRNCIHKCCYRRMYW 212
S+L LL L ++L S +C+ CC +W
Sbjct: 248 SILKDYLLNLVKTSESLSSQQMSCLRICCNSSSFW 282
>SPCC417.09c |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 767
Score = 24.6 bits (51), Expect = 5.7
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = -3
Query: 334 DVLDPARSVLASVLLELPVXD 272
D+LDPA + +AS++ LP D
Sbjct: 264 DILDPADNTVASLIHALPPKD 284
>SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 363
Score = 23.8 bits (49), Expect = 10.0
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = -1
Query: 240 ISAATAGCTGSCQVCA 193
+S T GCT C+ CA
Sbjct: 62 LSIKTGGCTEDCKYCA 77
Score = 23.8 bits (49), Expect = 10.0
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -2
Query: 341 TAGCTGSCKVCA 306
T GCT CK CA
Sbjct: 66 TGGCTEDCKYCA 77
Score = 23.8 bits (49), Expect = 10.0
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -1
Query: 114 TAGCTGSCKVCA 79
T GCT CK CA
Sbjct: 66 TGGCTEDCKYCA 77
>SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 516
Score = 23.8 bits (49), Expect = 10.0
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +2
Query: 104 HPAVAALMDTVPTVVMEVYHPPAVPV 181
HPAV+A T P+ +EVY + V
Sbjct: 254 HPAVSAFYVTCPSKGIEVYGQDQIAV 279
>SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 372
Score = 23.8 bits (49), Expect = 10.0
Identities = 7/24 (29%), Positives = 13/24 (54%), Gaps = 2/24 (8%)
Frame = -3
Query: 196 CLHQCYWNC--RWMIHFHHDCRNC 131
C H+ + C +W++ + H C C
Sbjct: 339 CKHEFHRPCIAKWIVDYRHACPTC 362
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,462,686
Number of Sequences: 5004
Number of extensions: 27506
Number of successful extensions: 76
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 60
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 76
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 134126124
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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