BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0152.Seq (399 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 82 3e-17 SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharo... 64 1e-11 SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosacchar... 57 9e-10 SPBC3E7.10 |fma1||methionine aminopeptidase Fma1 |Schizosaccharo... 35 0.005 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 29 0.27 SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subuni... 28 0.61 SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 2.5 SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 3.3 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 24 7.6 SPBC36.05c |clr6||histone deacetylase |Schizosaccharomyces pombe... 24 7.6 SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|c... 24 7.6 SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 24 10.0 SPBC11G11.05 |rpa34||DNA-directed RNA polymerase I complex subun... 24 10.0 >SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosaccharomyces pombe|chr 1|||Manual Length = 381 Score = 82.2 bits (194), Expect = 3e-17 Identities = 44/87 (50%), Positives = 57/87 (65%), Gaps = 2/87 (2%) Frame = +1 Query: 1 CEFGDKLVLEETNKVFKKEKDSKKGIAFSTCVSVNNCICHFSPIASEPD--YILKKGDLA 174 C GD+L+ E KV++ KD+ KGIAF T VS N+ H SP+ S+P+ LK GD+ Sbjct: 53 CVRGDELLNEAIKKVYRT-KDAYKGIAFPTAVSPNDMAAHLSPLKSDPEANLALKSGDVV 111 Query: 175 KIDLGAHIDGFIAVVAHTVVVGESEVS 255 KI LGAHIDGF ++VA T VV E V+ Sbjct: 112 KILLGAHIDGFASLVATTTVVSEEPVT 138 >SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 417 Score = 63.7 bits (148), Expect = 1e-11 Identities = 30/83 (36%), Positives = 52/83 (62%), Gaps = 6/83 (7%) Frame = +1 Query: 7 FGDKLVLEETNKVFKKEKDSKKGIAFSTCVSVNNCICHFSP------IASEPDYILKKGD 168 +GD L+ E + ++K ++ +KGIA T + VNNC +++P ++ Y L+ GD Sbjct: 51 YGDNLLHEYKSSIYKSQR-FEKGIAEPTSICVNNCAYNYAPGPESVIAGNDNSYHLQVGD 109 Query: 169 LAKIDLGAHIDGFIAVVAHTVVV 237 + KI +G H DG+ A+++HT+VV Sbjct: 110 VTKISMGLHFDGYTALISHTIVV 132 >SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 57.2 bits (132), Expect = 9e-10 Identities = 27/69 (39%), Positives = 42/69 (60%) Frame = +1 Query: 25 LEETNKVFKKEKDSKKGIAFSTCVSVNNCICHFSPIASEPDYILKKGDLAKIDLGAHIDG 204 +E T + +E K GI F T VS+N+C H++P A + ILK+ D+ K+D+G H++G Sbjct: 148 IENTTRALVEEDGLKSGIGFPTGVSLNHCAAHYTPNAGDTT-ILKEKDVMKVDIGVHVNG 206 Query: 205 FIAVVAHTV 231 I A T+ Sbjct: 207 RIVDSAFTM 215 >SPBC3E7.10 |fma1||methionine aminopeptidase Fma1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 34.7 bits (76), Expect = 0.005 Identities = 20/53 (37%), Positives = 27/53 (50%) Frame = +1 Query: 85 STCVSVNNCICHFSPIASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTVVVGE 243 S C SVN ICH P P L+ GD+ ID+ + +GF + T VG+ Sbjct: 181 SVCTSVNEIICHGIP-DQRP---LEDGDIVNIDVSLYHNGFHGDLNETYYVGD 229 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 29.1 bits (62), Expect = 0.27 Identities = 11/30 (36%), Positives = 22/30 (73%) Frame = -3 Query: 169 NLLFSECNPVRSQWAKSDKCSCSQKRMLKM 80 +L+ S+C V+ QW+ ++CSCS + +L++ Sbjct: 2135 DLICSKCKTVK-QWSLKERCSCSGEWVLQL 2163 >SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subunit Rad15|Schizosaccharomyces pombe|chr 1|||Manual Length = 772 Score = 27.9 bits (59), Expect = 0.61 Identities = 17/58 (29%), Positives = 28/58 (48%), Gaps = 3/58 (5%) Frame = -1 Query: 300 LH*HDELPTKHLQHVRDFTFTHNYCMSYNSYKSINVSTKIYLG*ISFF---QNVIRFA 136 LH E PT LQHV+D TF + + + + ++ + + + F Q V+ FA Sbjct: 346 LHVIAETPTSFLQHVKDITFIDKKPLRFCAERLTSLVRALQISLVEDFHSLQQVVAFA 403 >SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 357 Score = 25.8 bits (54), Expect = 2.5 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = -1 Query: 231 YCMSYNSYKSINVSTKIYLG*ISF 160 YC +YKS+ + T +Y+G F Sbjct: 144 YCRKVLTYKSLGIRTAVYVGAFCF 167 >SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 486 Score = 25.4 bits (53), Expect = 3.3 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +1 Query: 139 EPDYILKKGDLAKIDLGAHIDGFIAVVAHTVV 234 EP+Y+L G+L+ +L ID F T V Sbjct: 382 EPEYLLMYGNLSNEELVERIDAFYMNARRTYV 413 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 24.2 bits (50), Expect = 7.6 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = +1 Query: 7 FGDKLVLEETNKVFKKEKD 63 FG+KL E T K+ EKD Sbjct: 873 FGNKLAKEHTEKISSLEKD 891 >SPBC36.05c |clr6||histone deacetylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 405 Score = 24.2 bits (50), Expect = 7.6 Identities = 8/19 (42%), Positives = 13/19 (68%) Frame = -1 Query: 237 HNYCMSYNSYKSINVSTKI 181 HN ++YN Y+ +NV T + Sbjct: 36 HNLVVNYNLYEKLNVITPV 54 >SPAC890.06 |||nucleoporin Nup157/170|Schizosaccharomyces pombe|chr 1|||Manual Length = 1315 Score = 24.2 bits (50), Expect = 7.6 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -2 Query: 107 LFTETHVENAMPFLESFSFLKTLLVSSNTS 18 L+ T +EN F+E + TL+ SSN + Sbjct: 678 LYLSTFLENNKSFIEGLNSPNTLIGSSNVA 707 >SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 2244 Score = 23.8 bits (49), Expect = 10.0 Identities = 12/46 (26%), Positives = 22/46 (47%) Frame = +1 Query: 1 CEFGDKLVLEETNKVFKKEKDSKKGIAFSTCVSVNNCICHFSPIAS 138 C++ ++ TN+V E S+ G F ++ I H + +AS Sbjct: 1602 CDYNFSVIASSTNEVTISELTSESGCLFFHFEKDDSGIDHVTAVAS 1647 >SPBC11G11.05 |rpa34||DNA-directed RNA polymerase I complex subunit Rpa34 |Schizosaccharomyces pombe|chr 2|||Manual Length = 251 Score = 23.8 bits (49), Expect = 10.0 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +1 Query: 4 EFGDKLVLEETNKVFKKEKDSKKG 75 E GD V E NK +K+K+ KKG Sbjct: 203 ETGDATVEELQNKGKEKQKELKKG 226 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,518,220 Number of Sequences: 5004 Number of extensions: 27245 Number of successful extensions: 101 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 96 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 97 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 134126124 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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