BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0150.Seq (449 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC543.04 |||UPF0171 family protein|Schizosaccharomyces pombe|c... 27 1.3 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 25 5.4 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 7.1 SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 24 9.4 SPAC1D4.05c |||Erd1 homolog|Schizosaccharomyces pombe|chr 1|||Ma... 24 9.4 SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz... 24 9.4 >SPBC543.04 |||UPF0171 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 585 Score = 27.1 bits (57), Expect = 1.3 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +1 Query: 184 ILKLTSREITSNIPESLSRVTVPHH 258 ILKLT + + N+P + +PHH Sbjct: 218 ILKLTEKYSSQNVPFDVYAAQIPHH 242 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 25.0 bits (52), Expect = 5.4 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +3 Query: 123 GFSDTFKMPEIPEIKSLEFDDIKTHVA 203 GFSD ++ E P + +DDI +H A Sbjct: 919 GFSDINEIKEHPFFNGINWDDIFSHEA 945 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 24.6 bits (51), Expect = 7.1 Identities = 14/51 (27%), Positives = 24/51 (47%), Gaps = 4/51 (7%) Frame = +3 Query: 90 FVWEDDDDLFPGFSD--TFKMPEIPEIKSLEFDDIKTHV--AGDNEQYTGE 230 F WED+D+ G+ D + E+ E S F+ ++ DN+ + E Sbjct: 1943 FEWEDEDNASSGYEDELDYNEDEVGENDSTTFEAMENAFTETSDNDDHLEE 1993 >SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1463 Score = 24.2 bits (50), Expect = 9.4 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +3 Query: 9 YFPSTSLIMAFTKFLFVITLITIASAGFVW 98 YF + SL + F FLF+I I + VW Sbjct: 894 YFKACSLFLIFLYFLFIIGGIGMNVGTNVW 923 >SPAC1D4.05c |||Erd1 homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 387 Score = 24.2 bits (50), Expect = 9.4 Identities = 8/21 (38%), Positives = 14/21 (66%) Frame = -1 Query: 194 SFNIIKFKRLYLWYLWHLERI 132 SF +++ R +LW +HL+ I Sbjct: 365 SFEMLEILRRFLWLFFHLDAI 385 >SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schizosaccharomyces pombe|chr 1|||Manual Length = 307 Score = 24.2 bits (50), Expect = 9.4 Identities = 8/14 (57%), Positives = 11/14 (78%) Frame = +2 Query: 35 GVYEVSFCDYINYN 76 G+Y +FCD IN+N Sbjct: 155 GLYGFAFCDLINHN 168 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,615,617 Number of Sequences: 5004 Number of extensions: 28438 Number of successful extensions: 60 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 59 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 60 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 166231220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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