Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0144.Seq
(598 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 58 7e-10
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 36 0.004
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 32 0.055
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 0.90
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 28 1.2
SPBC1709.09 |||mitochondrial translation termination factor|Schi... 27 1.6
SPBC336.15 |pic1|SPBC685.01|INCENP-like|Schizosaccharomyces pomb... 27 2.7
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 3.6
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 26 3.6
SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|ch... 26 3.6
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 4.8
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 25 6.3
SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 25 6.3
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 25 6.3
SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb... 25 6.3
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 25 8.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.4
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 58.4 bits (135), Expect = 7e-10
Identities = 29/70 (41%), Positives = 40/70 (57%)
Frame = +3
Query: 255 KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGT 434
+P + K LVVQ+ V E+ ++CGG YLK+ + +M Y IMFGPD CG
Sbjct: 109 EPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGEMSNSIDYRIMFGPDKCGV-N 166
Query: 435 KKVHVIFSYK 464
+VH IF +K
Sbjct: 167 DRVHFIFKHK 176
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 35.9 bits (79), Expect = 0.004
Identities = 26/72 (36%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
Frame = -3
Query: 233 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHE-SSGNFSSKNTSQFIE 57
N SSE F S+S SL P VN + S + ++ L S + S+ NF+S ++S +
Sbjct: 475 NYTSSEAF---STSSSLVVIPPVNSSLVTSSTSFTKFSSLSSSQLSTENFTSASSSLSLT 531
Query: 56 DNASKLTTTSTT 21
+ S L+T STT
Sbjct: 532 NAKSSLSTPSTT 543
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 32.3 bits (70), Expect = 0.055
Identities = 21/64 (32%), Positives = 29/64 (45%)
Frame = +3
Query: 279 PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFS 458
P+V+ F VK + CG Y+ + + K++ E P I FG CG I S
Sbjct: 103 PIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKKCGMFDYISLSIIS 161
Query: 459 YKGK 470
Y GK
Sbjct: 162 YDGK 165
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 28.3 bits (60), Expect = 0.90
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = -3
Query: 164 NLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT 33
N N F + + S +SGNFS T Q DN S +T
Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFST 618
>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
Mug36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1646
Score = 27.9 bits (59), Expect = 1.2
Identities = 29/136 (21%), Positives = 54/136 (39%), Gaps = 1/136 (0%)
Frame = -3
Query: 455 EDYVYLLGSRTTNVRAEHNLIWSLSVHVLL-LQFAVKDLEVSASTVNVLFMFDSELDYQG 279
ED V G + + HNL+ S+ L ++D V T N+ +++LD+
Sbjct: 154 EDEVINEGLESEIDGSSHNLLDSILQKCLASTSVLMQDALVYIGTANMSTRLEAKLDFMS 213
Query: 278 FTLITERFNLRERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHES 99
F+ + R N + + +P+S S + + P + S S + ++
Sbjct: 214 FSSVKSNSTSRLLNINGITVSMVRPISKSNAGSSSPPRSEESFVSMDSSSSIVEGFENDL 273
Query: 98 SGNFSSKNTSQFIEDN 51
S + + N S I N
Sbjct: 274 SPSQVTLNESSIISSN 289
>SPBC1709.09 |||mitochondrial translation termination
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 244
Score = 27.5 bits (58), Expect = 1.6
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +1
Query: 181 FSDPEDDKGLKTSEDARFYALSRKLNRSVMRVNP 282
F D + LK ED+R+ ++ KLN S + V P
Sbjct: 130 FEDVDIKNILKAIEDSRYPFVANKLNASTIEVKP 163
>SPBC336.15 |pic1|SPBC685.01|INCENP-like|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1018
Score = 26.6 bits (56), Expect = 2.7
Identities = 14/50 (28%), Positives = 23/50 (46%)
Frame = +1
Query: 181 FSDPEDDKGLKTSEDARFYALSRKLNRSVMRVNPW*SSSLSNMNKTLTVE 330
+SD DD+G K + +A S +L + R W + M K L ++
Sbjct: 926 YSDDSDDEGNKKKVNLPSWAESPELREQLKRQQKWDPDKIFGMIKPLQMD 975
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 3.6
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = -3
Query: 233 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFP 138
N +E F+P + SL N P +N ++ ++ P
Sbjct: 685 NSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.2 bits (55), Expect = 3.6
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +1
Query: 85 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 228
+ F +D + +W EH K ++ + + FS+ DD+ + SE +
Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95
>SPAC12B10.08c |||mitochondrial tRNA|Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 26.2 bits (55), Expect = 3.6
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -1
Query: 304 LTVNWTTKGLPSLLNGLTYGREHRTSHLQR 215
L +NW + SL N T REHR L R
Sbjct: 89 LNINWGNDDVHSLTNLETIAREHRYQVLTR 118
>SPBC215.15 |sec13||COPII-coated vesicle component
Sec13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.8 bits (54), Expect = 4.8
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 82 EEKFPDDSWESNWVYS 129
EEKFPD +W +W S
Sbjct: 251 EEKFPDIAWRVSWSLS 266
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 25.4 bits (53), Expect = 6.3
Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 4/57 (7%)
Frame = -3
Query: 254 NLRERA*NLASSEV----FKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESS 96
+LR RA L+ E+ + LS+ LK +++ FP SFP +Y + ++SS
Sbjct: 122 DLRMRAIFLSIDEISMICIRILSAEERLKTGRSIDSGFPFSFPVHLIYDMCILNDSS 178
>SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1315
Score = 25.4 bits (53), Expect = 6.3
Identities = 12/31 (38%), Positives = 14/31 (45%)
Frame = -1
Query: 127 CTPNCFPTNHQETSPQRIHHNLSKIMPAS*Q 35
C P FP N+QE H+ I AS Q
Sbjct: 791 CKPESFPANYQEVLDYYTHNGFRVIACASKQ 821
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 25.4 bits (53), Expect = 6.3
Identities = 19/74 (25%), Positives = 32/74 (43%), Gaps = 4/74 (5%)
Frame = +3
Query: 327 GGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTK----KVHVIFSYKGKNHLIKKDI 494
G +LKV + E M ETP +I++ + G+ +H K + KK++
Sbjct: 614 GKKFLKVDEATREDMLMSAETPDDILWITTLISTGSSMSHFPLHAYLEAKERQEFTKKNL 673
Query: 495 RCKGCVYTHFVHSD 536
C F++SD
Sbjct: 674 -LLFCKEDEFLYSD 686
>SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 508
Score = 25.4 bits (53), Expect = 6.3
Identities = 14/48 (29%), Positives = 20/48 (41%)
Frame = +1
Query: 241 LSRKLNRSVMRVNPW*SSSLSNMNKTLTVEADTSRSLTANWSRRTCTE 384
L ++LN MRV W M K + +R + A R C+E
Sbjct: 173 LDKELNTYAMRVREWYGWHFPEMGKIIQDNLAYARVIKAMGMRTKCSE 220
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 25.0 bits (52), Expect = 8.4
Identities = 18/70 (25%), Positives = 33/70 (47%)
Frame = +1
Query: 226 ARFYALSRKLNRSVMRVNPW*SSSLSNMNKTLTVEADTSRSLTANWSRRTCTERLHMRLC 405
+R Y+L+ +++ ++ W LSN N L +E +ER++ RL
Sbjct: 3204 SREYSLANEMDLIKKKIIDWRKFELSNWNNLLKLE------------EYKLSERVYPRLY 3251
Query: 406 SALTFVVREP 435
S L F++ +P
Sbjct: 3252 SILQFIILKP 3261
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.0 bits (52), Expect = 8.4
Identities = 18/71 (25%), Positives = 35/71 (49%)
Frame = -3
Query: 227 ASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNA 48
+SS LSS S + + + ++F S ++ SH SS + SS ++ + ++
Sbjct: 395 SSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHNSTSSRSSSHSSSHSLSSLSSKPILTASS 454
Query: 47 SKLTTTSTTAF 15
S L T+S+ +
Sbjct: 455 SSLLTSSSHTY 465
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,616,467
Number of Sequences: 5004
Number of extensions: 56840
Number of successful extensions: 174
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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