BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0144.Seq
(598 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.) 81 5e-16
SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0) 44 1e-04
SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.043
SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.8
SB_45201| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.6
>SB_30637| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1137
Score = 81.4 bits (192), Expect = 5e-16
Identities = 36/63 (57%), Positives = 43/63 (68%)
Frame = +1
Query: 73 VFFEEKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRK 252
V F EKF D SWE WV S G + GKFK TAGKF+ D E DKG++TSEDA+FY +S K
Sbjct: 756 VHFLEKFEDKSWEDRWVSSTSKGAQQGKFKWTAGKFYGDAEADKGIQTSEDAKFYGISAK 815
Query: 253 LNR 261
+
Sbjct: 816 FEK 818
Score = 60.1 bits (139), Expect = 1e-09
Identities = 24/30 (80%), Positives = 28/30 (93%)
Frame = +3
Query: 255 KPFSNEGKPLVVQFTVKHEQDIDCGGGYLK 344
KPF+NEGK LV+QF+VKHEQ+IDCGGGY K
Sbjct: 818 KPFTNEGKTLVIQFSVKHEQNIDCGGGYAK 847
>SB_49315| Best HMM Match : Calreticulin (HMM E-Value=0)
Length = 1086
Score = 43.6 bits (98), Expect = 1e-04
Identities = 22/46 (47%), Positives = 29/46 (63%), Gaps = 1/46 (2%)
Frame = +3
Query: 354 CKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKG-KNHLIKK 488
C L Q+ +TPY IMFGPD CG +K+H IF +K KN I++
Sbjct: 28 CHLFQESFGDKTPYTIMFGPDKCGE-DRKLHFIFRHKNPKNGTIEE 72
>SB_29195| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 539
Score = 35.1 bits (77), Expect = 0.043
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = -1
Query: 331 PPQSMSCSCLTVNWTTKGLPSLLNG 257
P + SC CL V W T GLP L++G
Sbjct: 429 PSEQNSCQCLFVGWQTVGLPQLIHG 453
>SB_28048| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 125
Score = 28.7 bits (61), Expect = 3.8
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = -2
Query: 135 VLTVHPIAFPRIIRKLLLKEYI 70
+LT H + P++IRK+ LK+Y+
Sbjct: 73 LLTAHLVVAPKLIRKMPLKDYV 94
>SB_45201| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 522
Score = 27.9 bits (59), Expect = 6.6
Identities = 29/97 (29%), Positives = 38/97 (39%), Gaps = 4/97 (4%)
Frame = +3
Query: 261 FSNEGK---PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI-MFGPDICGP 428
FS GK L++ K + DCG + + D K + GE PYE G
Sbjct: 283 FSESGKLKRHLMIHTGEKPHKCDDCGKRFTQSGDLKTHLRIHTGEKPYECDDCGKRFTLS 342
Query: 429 GTKKVHVIFSYKGKNHLIKKDIRCKGCVYTHFVHSDL 539
G K HV K H KK +C C + H+ L
Sbjct: 343 GNMKKHV------KIHSGKKPYKCDECGKSFTEHAYL 373
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 19,786,777
Number of Sequences: 59808
Number of extensions: 456777
Number of successful extensions: 1068
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 993
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1065
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1439498375
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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