Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0144.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 120 1e-27
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 119 2e-27
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 115 2e-26
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 60 8e-10
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 59 3e-09
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 54 5e-08
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 0.58
At3g20150.1 68416.m02554 kinesin motor family protein contains P... 30 1.0
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 1.0
At5g56820.1 68418.m07090 F-box family protein contains F-box dom... 30 1.3
At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705... 29 2.4
At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kina... 29 2.4
At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5)... 29 3.1
At4g35760.1 68417.m05076 expressed protein 27 7.2
At1g33560.1 68414.m04153 disease resistance protein (CC-NBS-LRR ... 27 7.2
At5g41320.1 68418.m05022 expressed protein 27 9.5
At2g42290.1 68415.m05235 leucine-rich repeat family protein cont... 27 9.5
At1g74670.1 68414.m08647 gibberellin-responsive protein, putativ... 27 9.5
At1g62190.1 68414.m07015 expressed protein 27 9.5
At1g31910.1 68414.m03921 GHMP kinase family protein contains TIG... 27 9.5
At1g19100.1 68414.m02376 ATP-binding region, ATPase-like domain-... 27 9.5
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 120 bits (288), Expect = 1e-27
Identities = 52/87 (59%), Positives = 63/87 (72%)
Frame = +3
Query: 261 FSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKK 440
FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++QK G+TPY IMFGPDICG TKK
Sbjct: 87 FSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICGYSTKK 146
Query: 441 VHVIFSYKGKNHLIKKDIRCKGCVYTH 521
VH I +Y NHLIKKD+ C+ TH
Sbjct: 147 VHAILTYNEANHLIKKDVPCETDQLTH 173
Score = 58.0 bits (134), Expect = 4e-09
Identities = 31/60 (51%), Positives = 41/60 (68%), Gaps = 2/60 (3%)
Frame = +1
Query: 73 VFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYALS 246
V FEE+F DD WE+ WV SE + G++K TAG + D +DKG++TSED RFYA+S
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRFYAIS 81
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 119 bits (286), Expect = 2e-27
Identities = 51/87 (58%), Positives = 63/87 (72%)
Frame = +3
Query: 261 FSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKK 440
FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++Q G+TPY IMFGPDICG TKK
Sbjct: 87 FSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGPDICGYSTKK 146
Query: 441 VHVIFSYKGKNHLIKKDIRCKGCVYTH 521
VH I +Y G NHLIKK++ C+ TH
Sbjct: 147 VHAILTYNGTNHLIKKEVPCETDQLTH 173
Score = 58.4 bits (135), Expect = 3e-09
Identities = 30/64 (46%), Positives = 43/64 (67%), Gaps = 2/64 (3%)
Frame = +1
Query: 61 INCDVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARF 234
++ +V FEEKF +D WE WV S+ + G++K TAG + D +DKG++TSED RF
Sbjct: 20 VSAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRF 77
Query: 235 YALS 246
YA+S
Sbjct: 78 YAIS 81
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 115 bits (277), Expect = 2e-26
Identities = 49/91 (53%), Positives = 67/91 (73%)
Frame = +3
Query: 252 VKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPG 431
+ FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY +MFGPDICG
Sbjct: 90 IPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPDICGTQ 149
Query: 432 TKKVHVIFSYKGKNHLIKKDIRCKGCVYTHF 524
TKK+HVI SY+G+N+ IKKD++C+ HF
Sbjct: 150 TKKLHVIVSYQGQNYPIKKDLQCETDKLNHF 180
Score = 55.2 bits (127), Expect = 3e-08
Identities = 29/64 (45%), Positives = 41/64 (64%), Gaps = 2/64 (3%)
Frame = +1
Query: 70 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 243
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 244 SRKL 255
S K+
Sbjct: 87 SAKI 90
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 60.5 bits (140), Expect = 8e-10
Identities = 24/51 (47%), Positives = 36/51 (70%)
Frame = +3
Query: 252 VKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIM 404
+ FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY ++
Sbjct: 90 IPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVL 140
Score = 55.2 bits (127), Expect = 3e-08
Identities = 29/64 (45%), Positives = 41/64 (64%), Gaps = 2/64 (3%)
Frame = +1
Query: 70 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 243
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 244 SRKL 255
S K+
Sbjct: 87 SAKI 90
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 58.8 bits (136), Expect = 3e-09
Identities = 28/73 (38%), Positives = 43/73 (58%), Gaps = 3/73 (4%)
Frame = +3
Query: 255 KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICG 425
+P + + +V+Q+ V+ ++ ++CGG YLK + + E+PY IMFGPD CG
Sbjct: 85 EPLNLKEGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESPYSIMFGPDKCG 144
Query: 426 PGTKKVHVIFSYK 464
GT KVH I +K
Sbjct: 145 -GTNKVHFILKHK 156
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 54.4 bits (125), Expect = 5e-08
Identities = 27/69 (39%), Positives = 40/69 (57%), Gaps = 3/69 (4%)
Frame = +3
Query: 267 NEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGTK 437
NEG +V+Q+ + ++ ++CGG YLK + + ++PY IMFGPD CG T
Sbjct: 92 NEGT-VVLQYEARFQEGLECGGAYLKYLRPQEAGWVPQGFDNDSPYSIMFGPDKCG-ATN 149
Query: 438 KVHVIFSYK 464
KVH I +K
Sbjct: 150 KVHFILKHK 158
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 0.58
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +3
Query: 288 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 401
++F KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At3g20150.1 68416.m02554 kinesin motor family protein contains Pfam
domain, PF00225: Kinesin motor domain
Length = 1114
Score = 30.3 bits (65), Expect = 1.0
Identities = 24/76 (31%), Positives = 32/76 (42%), Gaps = 5/76 (6%)
Frame = -1
Query: 412 GPNIISYGVSPCMSFCS-SLQSKTLRYPPPQS----MSCSCLTVNWTTKGLPSLLNGLTY 248
G +II ++P + CS SL S L PP +S C T + LPS++ G Y
Sbjct: 693 GLDIIDNPMNPASNRCSVSLSSDNLTMQPPTDDRLPLSPLCPTCRICSSKLPSVVEGDGY 752
Query: 247 GREHRTSHLQRFSNLC 200
E Q LC
Sbjct: 753 HMEGVLEKQQELEKLC 768
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 1.0
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +3
Query: 288 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYE 398
++F KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At5g56820.1 68418.m07090 F-box family protein contains F-box domain
Pfam:PF00646
Length = 435
Score = 29.9 bits (64), Expect = 1.3
Identities = 17/64 (26%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
Frame = -3
Query: 188 SLKNFPAVNLNFPNSFPGC-SLYTQLLSHES-SGNFSSKNTSQFIEDNASKLTTTSTTAF 15
S+K+ P +++ PNS P C + + + L + +G K + +I NA +L T + + +
Sbjct: 332 SIKDQPNISVRKPNSVPECLTFHLETLEWQGYAGRPEDKEIAVYILGNALRLNTATISRY 391
Query: 14 IFDS 3
S
Sbjct: 392 FSSS 395
>At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705:
Eukaryotic protein of unknown function (DUF829)
Length = 420
Score = 29.1 bits (62), Expect = 2.4
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -1
Query: 376 MSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGL 254
+ F S L K +P + MS V+W KG+ S L+GL
Sbjct: 147 LDFTSDLNVKFALHPTIRRMSGPSRLVSWVAKGISSGLDGL 187
>At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 769
Score = 29.1 bits (62), Expect = 2.4
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3
Query: 116 LLSHESSGNFSSKNTSQFIEDNASKLTTTS 27
LLSH S+ N TS+ + + S+LTTTS
Sbjct: 218 LLSHNSTINIDDLRTSKAVSRSLSELTTTS 247
>At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5) /
gibberellin-responsive protein 5 identical to GASA5
[Arabidopsis thaliana] GI:1289320
Length = 97
Score = 28.7 bits (61), Expect = 3.1
Identities = 15/37 (40%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -1
Query: 382 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 281
PCM FC K L PP +C C NW TK
Sbjct: 55 PCMFFCLKCCKKCLCVPPGTFGNKQTCPCYN-NWKTK 90
>At4g35760.1 68417.m05076 expressed protein
Length = 376
Score = 27.5 bits (58), Expect = 7.2
Identities = 17/47 (36%), Positives = 23/47 (48%)
Frame = +1
Query: 223 DARFYALSRKLNRSVMRVNPW*SSSLSNMNKTLTVEADTSRSLTANW 363
D RF A+ K + S + SLS+ + + T E TS S T NW
Sbjct: 36 DRRFPAIPIKCSSSEPENGEDSAPSLSSSSSSSTSEVSTSNSSTYNW 82
>At1g33560.1 68414.m04153 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 787
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/53 (30%), Positives = 24/53 (45%)
Frame = +1
Query: 244 SRKLNRSVMRVNPW*SSSLSNMNKTLTVEADTSRSLTANWSRRTCTERLHMRL 402
+RKL V+R N W + + NK +E SR L + E H+R+
Sbjct: 76 ARKLCEKVLRCNRWNLKHVYHANKMKDLEKQISRFLNSQILLFVLAEVCHLRV 128
>At5g41320.1 68418.m05022 expressed protein
Length = 515
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -3
Query: 221 SEVFKPLSSSGSLKNFPAVNLNFPNSFPG 135
S +F LSSS L++FP + FP+S G
Sbjct: 366 SSLFPKLSSSSLLQSFPVPSSLFPSSSQG 394
>At2g42290.1 68415.m05235 leucine-rich repeat family protein
contains protein kinase domain, Pfam:PF00069; contains
leucine-rich repeats, Pfam:PF00560
Length = 646
Score = 27.1 bits (57), Expect = 9.5
Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 3/29 (10%)
Frame = -1
Query: 418 MSGPNIISYGVSP---CMSFCSSLQSKTL 341
M P+I+S VS CMSFCSSL S L
Sbjct: 1 MKLPSILSLVVSSIFLCMSFCSSLNSDGL 29
>At1g74670.1 68414.m08647 gibberellin-responsive protein, putative
similar to SP|P46690 Gibberellin-regulated protein 4
precursor {Arabidopsis thaliana} GASA4; contains Pfam
profile PF02704: Gibberellin regulated protein
Length = 101
Score = 27.1 bits (57), Expect = 9.5
Identities = 14/37 (37%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -1
Query: 382 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 281
PCM FC +K L PP C C NW T+
Sbjct: 59 PCMFFCQKCCAKCLCVPPGTYGNKQVCPCYN-NWKTQ 94
>At1g62190.1 68414.m07015 expressed protein
Length = 295
Score = 27.1 bits (57), Expect = 9.5
Identities = 10/37 (27%), Positives = 23/37 (62%)
Frame = -1
Query: 508 HPLQRISFLMRWFLPL*LKITCTFLVPGPQMSGPNII 398
+PL+ I+ + R + P +++TC+ PQ++ N++
Sbjct: 9 NPLRPITNIPRSYGPTRVRVTCSVTTTNPQLNHENLV 45
>At1g31910.1 68414.m03921 GHMP kinase family protein contains
TIGRFAM profile TIGR01219: phosphomevalonate kinase;
contains Pfam PF00288: GHMP kinases putative ATP-binding
protein domain; similar to Phosphomevalonate kinase (EC
2.7.4.2) (Swiss-Prot:P24521) [Saccharomyces cerevisiae]
Length = 505
Score = 27.1 bits (57), Expect = 9.5
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +1
Query: 151 GKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKLNRSV 267
GK +T G + + + GL S +ARFYA+ + +N V
Sbjct: 9 GKVLMTGGYLVLE-KPNAGLVLSTNARFYAIVKPINEEV 46
>At1g19100.1 68414.m02376 ATP-binding region, ATPase-like
domain-containing protein-related low similarity to
microrchidia [Homo sapiens] GI:5410257; contains
non-consensus splice site (GC) at intron 8
Length = 663
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/66 (18%), Positives = 32/66 (48%)
Frame = -3
Query: 542 SQIRVYKMCVDTSFAADIFFDEMVLTLVTEDYVYLLGSRTTNVRAEHNLIWSLSVHVLLL 363
+++ +Y M +++ ++ FD + ++ E + GS+ N +SL V++ +L
Sbjct: 303 TKVIIYNMWLNSDAKLELDFDSVAEDILIEGSIKKTGSKIVNDHIASRFSYSLRVYLSIL 362
Query: 362 QFAVKD 345
+ +
Sbjct: 363 YLRIPE 368
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,767,670
Number of Sequences: 28952
Number of extensions: 308279
Number of successful extensions: 824
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 791
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 817
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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