BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0140.Seq (439 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting... 26 2.9 SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomy... 25 5.1 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 24 8.9 >SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting complex subunit Apc4|Schizosaccharomyces pombe|chr 1|||Manual Length = 719 Score = 25.8 bits (54), Expect = 2.9 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = -3 Query: 257 CWNNRI*SRXYXVGFQNSEVMINRDNWGHSVLRR 156 CWN+ VGF+N E+ I + GH V +R Sbjct: 75 CWNHD--GNLIVVGFKNGELKIIDSSTGHLVEQR 106 >SPAC1093.05 |||ATP-dependent RNA helicase Hca4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 735 Score = 25.0 bits (52), Expect = 5.1 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = -2 Query: 165 IATLEVKFLDXRKTNISESICQRCFHQSRTKVXGXKA 55 IA + D +KT+I + CF + K G KA Sbjct: 405 IAVERINVKDGKKTSIRNQLQNLCFKDNDIKYIGQKA 441 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 24.2 bits (50), Expect = 8.9 Identities = 16/52 (30%), Positives = 25/52 (48%), Gaps = 1/52 (1%) Frame = +1 Query: 1 SPGLQEFXAAVRTXAXSNRLXTXNFRS*LMKTPLANAFADVR-LATIQEFHL 153 SP +Q F AVR+ R F + K PL N F ++ ++ Q F++ Sbjct: 3128 SPQVQWFWRAVRSFDEEERAKLLQFATGTSKVPL-NGFKELEGMSGFQRFNI 3178 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,236,864 Number of Sequences: 5004 Number of extensions: 17822 Number of successful extensions: 33 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 33 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 33 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 158122380 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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