Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0124.Seq
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 166 2e-42
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 84 1e-17
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 80 2e-16
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 75 5e-15
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 66 3e-12
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 56 3e-09
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 47 2e-06
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 45 6e-06
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 43 3e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 3.2
SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3 |Schizosaccha... 26 4.2
SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16 |Schizosa... 25 7.3
SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces p... 25 9.7
SPBC2G2.09c |crs1|mug17|meiosis specific cyclin Crs1|Schizosacch... 25 9.7
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 25 9.7
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 166 bits (404), Expect = 2e-42
Identities = 76/85 (89%), Positives = 81/85 (95%)
Frame = -2
Query: 508 DIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILA 329
DIRKDLY N V+SGGTTMYPGIADRMQKEI ALAPS+MK+KI+APPERKYSVWIGGSILA
Sbjct: 288 DIRKDLYGNVVMSGGTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSILA 347
Query: 328 SLSTFQQMWISKQEYDESGPSIVHR 254
SLSTFQQMWISKQEYDESGP IV+R
Sbjct: 348 SLSTFQQMWISKQEYDESGPGIVYR 372
Score = 31.5 bits (68), Expect = 0.085
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = -3
Query: 546 HETTYNSIMKCDV 508
HE TYNSIMKCDV
Sbjct: 275 HEATYNSIMKCDV 287
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 84.2 bits (199), Expect = 1e-17
Identities = 42/98 (42%), Positives = 61/98 (62%), Gaps = 3/98 (3%)
Frame = -2
Query: 538 HIQLHHEVRR---DIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPE 368
HIQL + D+R LY+N VLSGG+T+ G +R E+ A++ ++KI A PE
Sbjct: 280 HIQLFKSILLSDIDLRSTLYSNIVLSGGSTLLRGFGERFISELRAISGKKNQVKIYASPE 339
Query: 367 RKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 254
R ++ W+GGSILASLSTF+++ I+ +EY I R
Sbjct: 340 RMHNAWLGGSILASLSTFRRLLITSEEYKNDQNVIFRR 377
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 80.2 bits (189), Expect = 2e-16
Identities = 39/82 (47%), Positives = 56/82 (68%), Gaps = 3/82 (3%)
Frame = -2
Query: 508 DIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGS 338
DIR L N ++ GGT++ G + R+Q E++ L P + ++KI A ER Y+ W+GGS
Sbjct: 341 DIRASLLNNVIVCGGTSLMQGFSLRLQNELSKLYPGS-RLKIHASGHVVERSYASWLGGS 399
Query: 337 ILASLSTFQQMWISKQEYDESG 272
IL+SL TF Q+WIS+QEY+E G
Sbjct: 400 ILSSLGTFHQLWISRQEYEEHG 421
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 75.4 bits (177), Expect = 5e-15
Identities = 36/81 (44%), Positives = 52/81 (64%), Gaps = 2/81 (2%)
Frame = -2
Query: 508 DIRKDLYANTVLSGGTTMYPGIADRMQKEITALAP-STMKIKIIAPPER-KYSVWIGGSI 335
++R L N V++GGT++ PG+++R+Q E+ LA S + + +VW GGSI
Sbjct: 338 ELRSPLLNNIVVTGGTSLIPGLSERLQAEVQRLATGSRINVHTAETASATSNAVWFGGSI 397
Query: 334 LASLSTFQQMWISKQEYDESG 272
LASL FQ +W+SKQEYDE G
Sbjct: 398 LASLDNFQHLWVSKQEYDEVG 418
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 66.1 bits (154), Expect = 3e-12
Identities = 38/91 (41%), Positives = 57/91 (62%), Gaps = 12/91 (13%)
Frame = -2
Query: 508 DIRKDLYANTVLSGGTTMYPGIADRMQKEITAL--------APSTM---KIKIIAPPERK 362
DIRK LY VLSGG++MY G+ R++KEI L P+ + K+KI P R+
Sbjct: 288 DIRKYLYRAIVLSGGSSMYAGLPSRLEKEIKQLWFERVLHGDPARLPNFKVKIEDAPRRR 347
Query: 361 YSVWIGGSILAS-LSTFQQMWISKQEYDESG 272
++V+IGG++LA ++ MW+SK E++E G
Sbjct: 348 HAVFIGGAVLADIMAQNDHMWVSKAEWEEYG 378
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 56.4 bits (130), Expect = 3e-09
Identities = 30/99 (30%), Positives = 51/99 (51%), Gaps = 14/99 (14%)
Frame = -2
Query: 508 DIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPST--------------MKIKIIAPP 371
D+RK LY N VLSGG+T++ +R+Q+++ + + + +I+
Sbjct: 321 DVRKGLYKNIVLSGGSTLFKNFGNRLQRDLKRIVDERIHRSEMLSGAKSGGVDVNVISHK 380
Query: 370 ERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHR 254
++ +VW GGS+LA F +K +Y+E G SI R
Sbjct: 381 RQRNAVWFGGSLLAQTPEFGSYCHTKADYEEYGASIARR 419
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 46.8 bits (106), Expect = 2e-06
Identities = 20/81 (24%), Positives = 41/81 (50%)
Frame = -2
Query: 502 RKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASL 323
++ L +N +++GG PG+ R+++E+T++ P I + W G S +
Sbjct: 631 QQKLVSNVLITGGLGSLPGMETRIKRELTSIMPVGSSINVFRASNPLLDAWKGASEWSVT 690
Query: 322 STFQQMWISKQEYDESGPSIV 260
F+ ++++EY E GP +
Sbjct: 691 EKFKAAKVTREEYLEKGPEYI 711
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 45.2 bits (102), Expect = 6e-06
Identities = 25/85 (29%), Positives = 42/85 (49%)
Frame = -2
Query: 508 DIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILA 329
+I+ L N V GG +PG R+ E+ +LAP+ ++K+ P + W S +
Sbjct: 314 NIQAMLLENVVTIGGNCKFPGFHKRLSSELRSLAPANWEVKVFEPSDPICFPWKKASHM- 372
Query: 328 SLSTFQQMWISKQEYDESGPSIVHR 254
L + I++ EY E G +I+ R
Sbjct: 373 PLEHWNANKITRSEYSEHGANIMTR 397
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 43.2 bits (97), Expect = 3e-05
Identities = 19/83 (22%), Positives = 41/83 (49%), Gaps = 3/83 (3%)
Frame = -2
Query: 499 KDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPP---ERKYSVWIGGSILA 329
K+LY++ ++ GG +PG A +++ I + + I +I PP + ++ W G I
Sbjct: 527 KNLYSSILIVGGAGQFPGFAHLLEERIHSKRANIPTISVIPPPRSMDAQFVAWKGACIYN 586
Query: 328 SLSTFQQMWISKQEYDESGPSIV 260
+ ++WI ++ G ++
Sbjct: 587 RIRIVSELWIKNSDWKMLGSRVL 609
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 26.2 bits (55), Expect = 3.2
Identities = 11/35 (31%), Positives = 23/35 (65%), Gaps = 3/35 (8%)
Frame = -2
Query: 352 WIGGSILASLSTFQQM---WISKQEYDESGPSIVH 257
++GGSI+A S + + +++ +EY + GP+ +H
Sbjct: 486 FLGGSIVAKTSFNESVSSHYVTLEEYAQHGPTAIH 520
>SPAC16.04 |dus3||tRNA dihydrouridine synthase Dus3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 617
Score = 25.8 bits (54), Expect = 4.2
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -2
Query: 427 KEITALAPSTMKIKIIAPPERKYSVWIGGSILASLST 317
KEI++ A S + + + P E+ W ILA L+T
Sbjct: 212 KEISSQARSNIALPTLRPQEKNLIDWRDRKILAPLTT 248
>SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 25.0 bits (52), Expect = 7.3
Identities = 15/48 (31%), Positives = 18/48 (37%)
Frame = +2
Query: 149 LNTEFIYKITSVI*ATLLRFCCRAS*LKVSTLRSTSVYNGGTRLVVLL 292
LN F Y + A ++ C S L L TSV N LL
Sbjct: 416 LNIRFAYSFNDINQACVIEERCNFSSLSFGNLEQTSVNNDSEEFETLL 463
>SPAC56E4.04c |cut6||acetyl-CoA carboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2280
Score = 24.6 bits (51), Expect = 9.7
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -2
Query: 325 LSTFQQMWISKQEYDESGPSIV 260
L + + W KQ++DES S+V
Sbjct: 2195 LQKWYEEWCGKQDWDESDKSVV 2216
>SPBC2G2.09c |crs1|mug17|meiosis specific cyclin
Crs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 229
Score = 24.6 bits (51), Expect = 9.7
Identities = 9/21 (42%), Positives = 16/21 (76%)
Frame = +2
Query: 50 TLINKNLCQKKKRQQQHSVRF 112
+++N+N+ K+KRQQ SV +
Sbjct: 75 SILNQNIAYKEKRQQLFSVLY 95
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 24.6 bits (51), Expect = 9.7
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +2
Query: 293 FRDPHLLEGREGGEDRSTDPYGVLPLW 373
F HLL + G+D + + YG L W
Sbjct: 797 FHQGHLLYLSDEGKDETLERYGTLSSW 823
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,255,333
Number of Sequences: 5004
Number of extensions: 44588
Number of successful extensions: 143
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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