Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0123.Seq
         (548 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   105   3e-24
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   105   3e-24
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   105   3e-24
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    46   5e-06
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.064
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar...    27   1.8  
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe...    25   7.3  
SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|...    25   9.7  
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb...    25   9.7  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  105 bits (253), Expect = 3e-24
 Identities = 50/78 (64%), Positives = 60/78 (76%), Gaps = 1/78 (1%)
 Frame = -3

Query: 525 HCPH-CLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGR 349
           H  H   KFAE+  K+DR +GK  E +PK +KS DA I  +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421

Query: 348 FAVRDMRQTVAVGVIKAV 295
           FAVRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439



 Score = 32.7 bits (71), Expect = 0.037
 Identities = 12/13 (92%), Positives = 13/13 (100%)
 Frame = -1

Query: 548 YTPVLDCHTAHIA 510
           Y+PVLDCHTAHIA
Sbjct: 355 YSPVLDCHTAHIA 367


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  105 bits (253), Expect = 3e-24
 Identities = 50/78 (64%), Positives = 60/78 (76%), Gaps = 1/78 (1%)
 Frame = -3

Query: 525 HCPH-CLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGR 349
           H  H   KFAE+  K+DR +GK  E +PK +KS DA I  +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421

Query: 348 FAVRDMRQTVAVGVIKAV 295
           FAVRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439



 Score = 32.7 bits (71), Expect = 0.037
 Identities = 12/13 (92%), Positives = 13/13 (100%)
 Frame = -1

Query: 548 YTPVLDCHTAHIA 510
           Y+PVLDCHTAHIA
Sbjct: 355 YSPVLDCHTAHIA 367


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  105 bits (253), Expect = 3e-24
 Identities = 50/78 (64%), Positives = 60/78 (76%), Gaps = 1/78 (1%)
 Frame = -3

Query: 525 HCPH-CLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGR 349
           H  H   KFAE+  K+DR +GK  E +PK +KS DA I  +VPSKP+CVE+F ++ PLGR
Sbjct: 362 HTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGR 421

Query: 348 FAVRDMRQTVAVGVIKAV 295
           FAVRDMRQTVAVGVIKAV
Sbjct: 422 FAVRDMRQTVAVGVIKAV 439



 Score = 32.7 bits (71), Expect = 0.037
 Identities = 12/13 (92%), Positives = 13/13 (100%)
 Frame = -1

Query: 548 YTPVLDCHTAHIA 510
           Y+PVLDCHTAHIA
Sbjct: 355 YSPVLDCHTAHIA 367


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 45.6 bits (103), Expect = 5e-06
 Identities = 24/74 (32%), Positives = 42/74 (56%), Gaps = 1/74 (1%)
 Frame = -3

Query: 510 LKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDM 331
           + FA++  K+D+ T + ++  P         I  L    P+C+E F+++  +GRF +RD 
Sbjct: 590 VSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQ 648

Query: 330 RQTVAVG-VIKAVN 292
             TVAVG V+K ++
Sbjct: 649 GTTVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.064
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = -3

Query: 393 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 301
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPAC23A1.04c |mnl1||alpha mannosidase-like
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 787

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 12/47 (25%), Positives = 21/47 (44%)
 Frame = +1

Query: 406 QVDNGCIXRLDGFWVDFSRFTSTTVNFXFDFCKFEAMWAVWQSNTGV 546
           +V   C        ++FS  T+ T N  F      A ++VW+  +G+
Sbjct: 198 EVTENCAAAASSLVLEFSMLTALTGNNKFKASAENAFFSVWKRRSGI 244


>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 972

 Score = 25.0 bits (52), Expect = 7.3
 Identities = 13/32 (40%), Positives = 14/32 (43%)
 Frame = -1

Query: 170 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 75
           G S E     S+   IFY       FYNL KV
Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793


>SPAC25H1.07 |||DUF1620 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 885

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 11/35 (31%), Positives = 20/35 (57%)
 Frame = -1

Query: 236 QEVARAVNSTIFHTTAILHSPKGVSKEKRATNSFL 132
           + +A A+N +I  TT +    K +  E+ ++ SFL
Sbjct: 369 EALAYAINPSILPTTLLTSYQKSIQDEENSSVSFL 403


>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 631

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 11/23 (47%), Positives = 14/23 (60%)
 Frame = +2

Query: 443 FGLTSVDLPVXRSTXSLISANLR 511
           FGL  +  PV RST +L+  N R
Sbjct: 352 FGLPIIVAPVIRSTQTLLDKNYR 374


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,021,506
Number of Sequences: 5004
Number of extensions: 35278
Number of successful extensions: 98
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)