Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0123.Seq
(548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 102 2e-22
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 102 2e-22
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 102 2e-22
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 102 2e-22
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 95 2e-20
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 49 2e-06
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 47 1e-05
At4g27900.2 68417.m04005 expressed protein 29 2.0
At4g27900.1 68417.m04004 expressed protein 29 2.0
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 2.7
At5g42860.1 68418.m05224 expressed protein 25 4.4
At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 28 4.7
At2g04500.1 68415.m00455 DC1 domain-containing protein contains ... 27 8.3
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 102 bits (244), Expect = 2e-22
Identities = 47/79 (59%), Positives = 61/79 (77%), Gaps = 1/79 (1%)
Frame = -3
Query: 525 HCPH-CLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGR 349
H H +KF+EI K+DR +GK E PK +K+ DA +V + P+KP+ VE+F E+PPLGR
Sbjct: 352 HTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGR 411
Query: 348 FAVRDMRQTVAVGVIKAVN 292
FAVRDMRQTVAVGVIK+V+
Sbjct: 412 FAVRDMRQTVAVGVIKSVD 430
Score = 31.1 bits (67), Expect = 0.51
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = -1
Query: 548 YTPVLDCHTAHIA 510
Y PVLDCHT+HIA
Sbjct: 345 YAPVLDCHTSHIA 357
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 102 bits (244), Expect = 2e-22
Identities = 47/79 (59%), Positives = 61/79 (77%), Gaps = 1/79 (1%)
Frame = -3
Query: 525 HCPH-CLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGR 349
H H +KF+EI K+DR +GK E PK +K+ DA +V + P+KP+ VE+F E+PPLGR
Sbjct: 352 HTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGR 411
Query: 348 FAVRDMRQTVAVGVIKAVN 292
FAVRDMRQTVAVGVIK+V+
Sbjct: 412 FAVRDMRQTVAVGVIKSVD 430
Score = 31.1 bits (67), Expect = 0.51
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = -1
Query: 548 YTPVLDCHTAHIA 510
Y PVLDCHT+HIA
Sbjct: 345 YAPVLDCHTSHIA 357
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 102 bits (244), Expect = 2e-22
Identities = 47/79 (59%), Positives = 61/79 (77%), Gaps = 1/79 (1%)
Frame = -3
Query: 525 HCPH-CLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGR 349
H H +KF+EI K+DR +GK E PK +K+ DA +V + P+KP+ VE+F E+PPLGR
Sbjct: 352 HTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGR 411
Query: 348 FAVRDMRQTVAVGVIKAVN 292
FAVRDMRQTVAVGVIK+V+
Sbjct: 412 FAVRDMRQTVAVGVIKSVD 430
Score = 31.1 bits (67), Expect = 0.51
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = -1
Query: 548 YTPVLDCHTAHIA 510
Y PVLDCHT+HIA
Sbjct: 345 YAPVLDCHTSHIA 357
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 102 bits (244), Expect = 2e-22
Identities = 47/79 (59%), Positives = 61/79 (77%), Gaps = 1/79 (1%)
Frame = -3
Query: 525 HCPH-CLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGR 349
H H +KF+EI K+DR +GK E PK +K+ DA +V + P+KP+ VE+F E+PPLGR
Sbjct: 352 HTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPPLGR 411
Query: 348 FAVRDMRQTVAVGVIKAVN 292
FAVRDMRQTVAVGVIK+V+
Sbjct: 412 FAVRDMRQTVAVGVIKSVD 430
Score = 31.1 bits (67), Expect = 0.51
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = -1
Query: 548 YTPVLDCHTAHIA 510
Y PVLDCHT+HIA
Sbjct: 345 YAPVLDCHTSHIA 357
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 95.5 bits (227), Expect = 2e-20
Identities = 43/78 (55%), Positives = 58/78 (74%), Gaps = 1/78 (1%)
Frame = -3
Query: 525 HCPH-CLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGR 349
H H +KF+EI K+D TG E PK +K+ +AAI+N+ P+KP+ VE++ +PPLGR
Sbjct: 18 HTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTPTKPMVVEAYSAYPPLGR 77
Query: 348 FAVRDMRQTVAVGVIKAV 295
FA+RDMRQTV VGVIK+V
Sbjct: 78 FAIRDMRQTVGVGVIKSV 95
Score = 33.1 bits (72), Expect = 0.13
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = -1
Query: 548 YTPVLDCHTAHIA 510
YTPVLDCHT+HIA
Sbjct: 11 YTPVLDCHTSHIA 23
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 49.2 bits (112), Expect = 2e-06
Identities = 21/65 (32%), Positives = 37/65 (56%)
Frame = -3
Query: 498 EIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTV 319
E+K ++D T K + +K+ A + + + +C+E F +FP LGRF +R +T+
Sbjct: 458 ELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFSDFPQLGRFTLRTEGKTI 517
Query: 318 AVGVI 304
AVG +
Sbjct: 518 AVGKV 522
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 46.8 bits (106), Expect = 1e-05
Identities = 21/77 (27%), Positives = 41/77 (53%)
Frame = -3
Query: 525 HCPHCLKFAEIKXKVDRXTGKSTEVNPKSIKSXDAAIVNLVPSKPLCVESFQEFPPLGRF 346
H ++ +D TG+ T+ +P+ + + +A++ + P+CVE+F E LGR
Sbjct: 587 HAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRALGRV 646
Query: 345 AVRDMRQTVAVGVIKAV 295
+R +TVA+G + +
Sbjct: 647 FLRSSGRTVAMGKVTRI 663
>At4g27900.2 68417.m04005 expressed protein
Length = 261
Score = 29.1 bits (62), Expect = 2.0
Identities = 10/31 (32%), Positives = 20/31 (64%)
Frame = -3
Query: 447 PKSIKSXDAAIVNLVPSKPLCVESFQEFPPL 355
PKS+ S + + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At4g27900.1 68417.m04004 expressed protein
Length = 261
Score = 29.1 bits (62), Expect = 2.0
Identities = 10/31 (32%), Positives = 20/31 (64%)
Frame = -3
Query: 447 PKSIKSXDAAIVNLVPSKPLCVESFQEFPPL 355
PKS+ S + + +++V K ++ F +FPP+
Sbjct: 144 PKSVSSGNLSSMDMVEHKDAVIQGFPDFPPV 174
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 28.7 bits (61), Expect = 2.7
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -1
Query: 125 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 27
I+ K C+ V LFY ++ N S FCY C+L+C
Sbjct: 619 IYLKPCHIFKVGLFYKEVEIARNDGNSRLFCYICRLRC 656
>At5g42860.1 68418.m05224 expressed protein
Length = 320
Score = 25.0 bits (52), Expect(2) = 4.4
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = -1
Query: 254 RKGHQGQEVARAVNSTIFHTTAILHSPKGVSKEKRATNS 138
R H G++ A T FH+T +L SP G +++S
Sbjct: 33 RDSHDGEKTA-----TSFHSTPVLTSPMGSPPHSHSSSS 66
Score = 21.4 bits (43), Expect(2) = 4.4
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -1
Query: 398 PSLYV*SPSRNSH 360
P+ +V SPSR+SH
Sbjct: 24 PAYFVQSPSRDSH 36
>At1g55380.1 68414.m06334 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 661
Score = 27.9 bits (59), Expect = 4.7
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -1
Query: 125 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 27
I+ K C+ V L+Y ++ N S FCY C+L+C
Sbjct: 579 IYLKPCHIFKVGLYYKEVEIARNDGNSRLFCYTCELRC 616
>At2g04500.1 68415.m00455 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 495
Score = 27.1 bits (57), Expect = 8.3
Identities = 7/17 (41%), Positives = 13/17 (76%)
Frame = -1
Query: 71 HNISETFCYDCKLKCKF 21
+ ++ FC+ C+L+CKF
Sbjct: 444 NGVTRPFCFHCELRCKF 460
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,473,436
Number of Sequences: 28952
Number of extensions: 182395
Number of successful extensions: 479
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 460
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 479
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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