BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0121.Seq (459 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3H5.11 |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1||... 37 0.001 SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3... 36 0.002 SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces po... 36 0.002 SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1|... 31 0.085 SPAC25B8.01 |dap1|SPAC26H5.15|cytochrome P450 regulator Dap1|Sch... 25 4.2 SPBC25B2.06c |btb2||BTB/POZ domain protein Btb2|Schizosaccharomy... 25 4.2 SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 25 4.2 SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|... 25 4.2 SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc... 25 5.6 SPBC36B7.06c |mug20||sequence orphan|Schizosaccharomyces pombe|c... 25 7.3 SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|ch... 24 9.7 SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr... 24 9.7 SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 24 9.7 SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces ... 24 9.7 SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|ch... 24 9.7 >SPAC3H5.11 |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 393 Score = 37.1 bits (82), Expect = 0.001 Identities = 20/66 (30%), Positives = 32/66 (48%) Frame = +3 Query: 207 GVTGTLXDASSLFPAIVPP*WHSTWARWGFLTPFEFNNFQEQVMNVLEGHAALTLRSRLQ 386 G GT+ S LF VPP GFLT F+ ++ ++ + + LR+R + Sbjct: 148 GGDGTVLYTSRLFQRTVPPIMPFAMGTLGFLTHFDVKKYKTSILEIC-NEMYVHLRTRFE 206 Query: 387 CVVLRK 404 C V++K Sbjct: 207 CRVMKK 212 Score = 28.7 bits (61), Expect = 0.45 Identities = 23/98 (23%), Positives = 42/98 (42%) Frame = +2 Query: 2 DAQILAPFVQLVHWLVHDKSMVVFVEAAVLDDTLLAEYGDFTSVKERLMTFRASTDDLTD 181 D ++ + V+WL+ ++ VF++ ++ D L E + L+ + S Sbjct: 88 DEEVEEKLKEFVYWLISLDNITVFIQKSMED---LFEKTEKIQYWTTLLCTKHS-----Q 139 Query: 182 KIDFIICLGGDRDPXGC*LTFSSNRAAVMAFHLGSLGF 295 D ++ LGGD F +M F +G+LGF Sbjct: 140 LFDLVLTLGGDGTVLYTSRLFQRTVPPIMPFAMGTLGF 177 >SPCC24B10.02c |||NAD/NADH kinase|Schizosaccharomyces pombe|chr 3|||Manual Length = 449 Score = 36.3 bits (80), Expect = 0.002 Identities = 25/62 (40%), Positives = 31/62 (50%), Gaps = 1/62 (1%) Frame = +3 Query: 207 GVTGTLXDASSLFPAIVPP*WH-STWARWGFLTPFEFNNFQEQVMNVLEGHAALTLRSRL 383 G TL S LF I PP S GFLT F +N+Q+ + VL + +L SRL Sbjct: 182 GDNSTLLYTSWLFQKIGPPVLSFSDDDVPGFLTHFSLSNYQQHLYQVLTQNVSLRFCSRL 241 Query: 384 QC 389 QC Sbjct: 242 QC 243 >SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 361 Score = 36.3 bits (80), Expect = 0.002 Identities = 21/72 (29%), Positives = 36/72 (50%), Gaps = 1/72 (1%) Frame = +3 Query: 207 GVTGTLXDASSLFPAI-VPP*WHSTWARWGFLTPFEFNNFQEQVMNVLEGHAALTLRSRL 383 G GT+ A+SLF +PP + GFL PF+F +FQ + + + +R RL Sbjct: 132 GGDGTILHAASLFARSGMPPILSFSLGTLGFLLPFDFGSFQTAFADFYNSRSFVLMRMRL 191 Query: 384 QCVVLRKSQDDN 419 + + K +++ Sbjct: 192 RVAMKTKLYNES 203 Score = 30.7 bits (66), Expect = 0.11 Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 1/43 (2%) Frame = +2 Query: 170 DLTDKIDFIICLGGDRDPXGC*LTFS-SNRAAVMAFHLGSLGF 295 DL K+D II +GGD F+ S +++F LG+LGF Sbjct: 120 DLEQKVDAIITVGGDGTILHAASLFARSGMPPILSFSLGTLGF 162 >SPAC1B1.02c |||NAD/NADH kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 31.1 bits (67), Expect = 0.085 Identities = 17/57 (29%), Positives = 28/57 (49%) Frame = +3 Query: 231 ASSLFPAIVPP*WHSTWARWGFLTPFEFNNFQEQVMNVLEGHAALTLRSRLQCVVLR 401 AS LF +VPP + A+ GFL+ + + + + + LR R QC ++R Sbjct: 294 ASWLFQDVVPPVLSFSTAKAGFLSILPIAEYTKTLDLIFHRGFTVNLRMRFQCSIMR 350 >SPAC25B8.01 |dap1|SPAC26H5.15|cytochrome P450 regulator Dap1|Schizosaccharomyces pombe|chr 1|||Manual Length = 166 Score = 25.4 bits (53), Expect = 4.2 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = -1 Query: 303 ASENPNEPKWNAITAARLLE 244 ASE P +P+W T A L E Sbjct: 31 ASEEPKQPEWRDYTPAELKE 50 >SPBC25B2.06c |btb2||BTB/POZ domain protein Btb2|Schizosaccharomyces pombe|chr 2|||Manual Length = 284 Score = 25.4 bits (53), Expect = 4.2 Identities = 16/55 (29%), Positives = 25/55 (45%) Frame = -3 Query: 172 IVGASSESHQTFLHRRKVPILRKECVIQNCRLDEHHHAFVMNEPVY*LNEGCQYL 8 I G + H FL R P+L ++ + N + D H+ V E Y E C ++ Sbjct: 43 IKGETYHLHALFLGRS--PVLLQKLIENNPKGDVHYTIEVETEDPYVTKESCLFV 95 >SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 538 Score = 25.4 bits (53), Expect = 4.2 Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 3/52 (5%) Frame = +2 Query: 131 VKERLMTFRASTDDLTDKIDFIICLGGDR---DPXGC*LTFSSNRAAVMAFH 277 V+ ++ + +++ DKI + L GDR D SSN +AV AF+ Sbjct: 257 VRREMVVEKGLKEEVADKIKEYVLLKGDRSLLDKLEADSLLSSNSSAVAAFN 308 >SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 25.4 bits (53), Expect = 4.2 Identities = 13/40 (32%), Positives = 18/40 (45%) Frame = +3 Query: 150 LSELAPTISLIRSTSSYAWGVTGTLXDASSLFPAIVPP*W 269 L E P + I + YA+ + D S+L VPP W Sbjct: 179 LKEFLPYVDAIGTPEGYAFVILYKKVDQSALSKLPVPPGW 218 >SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 940 Score = 25.0 bits (52), Expect = 5.6 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = +2 Query: 167 DDLTDKIDFIICLGGDRDPXGC 232 +DL K+D I+ L G DP C Sbjct: 707 EDLASKLDHIVQLAGFTDPVYC 728 >SPBC36B7.06c |mug20||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 169 Score = 24.6 bits (51), Expect = 7.3 Identities = 12/39 (30%), Positives = 22/39 (56%) Frame = -3 Query: 187 DLISEIVGASSESHQTFLHRRKVPILRKECVIQNCRLDE 71 +++SE V A SE H + LH + K+ ++++ R E Sbjct: 18 NMVSEAVLAFSEHHDSALHSYFLNARVKDVILESLRTTE 56 >SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 525 Score = 24.2 bits (50), Expect = 9.7 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = -3 Query: 265 HGGTIAGKSELASXRVPVTPQAY 197 +G I +S+LAS RVP+ A+ Sbjct: 503 YGDRIRARSKLASQRVPINTAAH 525 >SPBPB7E8.02 |||PSP1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 749 Score = 24.2 bits (50), Expect = 9.7 Identities = 11/22 (50%), Positives = 11/22 (50%) Frame = -2 Query: 296 KTPTSPSGMPSRRHDCWKK*AS 231 K PS MPS H W K AS Sbjct: 131 KASLPPSEMPSHFHPLWSKSAS 152 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 24.2 bits (50), Expect = 9.7 Identities = 13/38 (34%), Positives = 16/38 (42%) Frame = -2 Query: 131 PT*SPHTPQGVCHPKLPPRRTPPCFCHERASVLAERRV 18 P S ++ G LPP PP F E A L E + Sbjct: 16 PFESENSSLGATPLSLPPSSPPPEFSDEAAEALVEEDI 53 >SPAC9G1.06c |cyk3||cytokinesis protein Cyk3|Schizosaccharomyces pombe|chr 1|||Manual Length = 886 Score = 24.2 bits (50), Expect = 9.7 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = +2 Query: 245 SSNRAAVMAFHLGSLGFSDALR 310 ++NRAAV+ + G LG S +R Sbjct: 735 ANNRAAVLRIYAGRLGVSSPVR 756 >SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 24.2 bits (50), Expect = 9.7 Identities = 8/15 (53%), Positives = 11/15 (73%) Frame = +3 Query: 81 RQFWMTHSLRSMGTL 125 R FW+ H L+S G+L Sbjct: 284 RHFWLLHGLKSHGSL 298 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,802,925 Number of Sequences: 5004 Number of extensions: 33177 Number of successful extensions: 107 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 101 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 107 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 172312850 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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