Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0114.Seq
(648 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 150 2e-37
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 150 2e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 150 2e-37
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 51 2e-07
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 32 0.082
SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 4.1
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 26 5.4
SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr 3|||Ma... 25 7.1
SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr 1|||Ma... 25 9.4
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 25 9.4
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 9.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 150 bits (363), Expect = 2e-37
Identities = 75/127 (59%), Positives = 88/127 (69%)
Frame = -2
Query: 632 GDSKNNPPKGAADFTAQVIVL*PSWSNLKRLHTSLGLPHCPLACKFAEIKEKVDRRTGKS 453
GDSKN+PP G A FTAQVI+L L +ACKFAE+ EK+DRR+GK
Sbjct: 325 GDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKK 384
Query: 452 TEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEA 273
E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 385 IEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAP 444
Query: 272 GGGKVTK 252
G KVTK
Sbjct: 445 GAAKVTK 451
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 150 bits (363), Expect = 2e-37
Identities = 75/127 (59%), Positives = 88/127 (69%)
Frame = -2
Query: 632 GDSKNNPPKGAADFTAQVIVL*PSWSNLKRLHTSLGLPHCPLACKFAEIKEKVDRRTGKS 453
GDSKN+PP G A FTAQVI+L L +ACKFAE+ EK+DRR+GK
Sbjct: 325 GDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKK 384
Query: 452 TEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEA 273
E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 385 IEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAP 444
Query: 272 GGGKVTK 252
G KVTK
Sbjct: 445 GAAKVTK 451
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 150 bits (363), Expect = 2e-37
Identities = 75/127 (59%), Positives = 88/127 (69%)
Frame = -2
Query: 632 GDSKNNPPKGAADFTAQVIVL*PSWSNLKRLHTSLGLPHCPLACKFAEIKEKVDRRTGKS 453
GDSKN+PP G A FTAQVI+L L +ACKFAE+ EK+DRR+GK
Sbjct: 325 GDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGKK 384
Query: 452 TEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEA 273
E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 385 IEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAVEKVAP 444
Query: 272 GGGKVTK 252
G KVTK
Sbjct: 445 GAAKVTK 451
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 50.8 bits (116), Expect = 2e-07
Identities = 37/121 (30%), Positives = 61/121 (50%), Gaps = 7/121 (5%)
Frame = -2
Query: 626 SKNNPPKGAADFTAQVIVL*-PS-----WSNLKRLHTSLGLPHCPLACKFAEIKEKVDRR 465
S NP F AQ+ +L PS +S + +HT++ FA++ K+D+
Sbjct: 549 STKNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVE------EVSFAKLLHKLDK- 601
Query: 464 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAV 288
T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG V+K +
Sbjct: 602 TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKIL 661
Query: 287 N 285
+
Sbjct: 662 D 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 31.9 bits (69), Expect = 0.082
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -2
Query: 386 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 294
PLC+ +E P LGRF +R TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 325
Score = 26.2 bits (55), Expect = 4.1
Identities = 19/51 (37%), Positives = 23/51 (45%)
Frame = +1
Query: 397 TKLTMAASPDLMDFGLTSVDLPVRRSTFSLISANLQASGQCGNPRLVCNRL 549
T L+ AA P L D TSV + + SL SA A P +VC L
Sbjct: 256 TSLSEAALPPLTDVQRTSVLSLLTSTLHSLESARQIADSNTWKPIIVCLEL 306
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.8 bits (54), Expect = 5.4
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = +1
Query: 409 MAASPDLMDFGLTSVDLPVRRSTFSLISANLQASGQCGNPRLVCNRLRFDQDG 567
++ +PDL D L+SVD P++ +T S + GN L+C+ F++DG
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS----------ESGNQYLLCD---FNRDG 66
>SPCC777.03c |||nifs homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 396
Score = 25.4 bits (53), Expect = 7.1
Identities = 19/72 (26%), Positives = 33/72 (45%), Gaps = 1/72 (1%)
Frame = +1
Query: 340 KRPRGGNSWKDSTHRGLEGTKLTMAASPDLMDFGLTSVDLPVRRSTFSL-ISANLQASGQ 516
+ P G W GLE + + S D+ ++ + V T ++ IS+ + SGQ
Sbjct: 116 EHPNHGFGWISLQEAGLE---VRLIPSKDIYYADASTFEPYVDEKTKAIGISSVMFHSGQ 172
Query: 517 CGNPRLVCNRLR 552
N + +CN+ R
Sbjct: 173 LNNVKDICNKFR 184
>SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 899
Score = 25.0 bits (52), Expect = 9.4
Identities = 14/47 (29%), Positives = 22/47 (46%)
Frame = -2
Query: 563 SWSNLKRLHTSLGLPHCPLACKFAEIKEKVDRRTGKSTEVNPKSIKS 423
S +N LH + L + K +EIKE D + E+ K ++S
Sbjct: 792 SEANAPELHNPVNLNYDAQLSKISEIKENYDELLTRYRELEGKFLES 838
>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 972
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/32 (40%), Positives = 14/32 (43%)
Frame = -3
Query: 163 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKV 68
G S E S+ IFY FYNL KV
Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKV 793
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +1
Query: 256 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 378
+ L P +S + TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,625,531
Number of Sequences: 5004
Number of extensions: 51870
Number of successful extensions: 143
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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