Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0110.Seq
(618 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14770.2 68414.m01766 expressed protein 36 0.022
At1g14770.1 68414.m01765 expressed protein 36 0.022
At2g22795.1 68415.m02704 expressed protein 31 0.61
At3g48860.2 68416.m05337 expressed protein 30 1.1
At3g48860.1 68416.m05336 expressed protein 30 1.1
At1g23280.1 68414.m02912 MAK16 protein-related contains similari... 30 1.1
At1g75310.1 68414.m08748 DNAJ heat shock N-terminal domain-conta... 29 1.9
At3g12810.1 68416.m01598 SNF2 domain-containing protein / helica... 29 2.5
At5g20680.1 68418.m02456 expressed protein predicted proteins, A... 29 3.3
At3g53670.1 68416.m05927 expressed protein 28 4.3
At3g10070.1 68416.m01207 transcription initiation factor IID (TF... 28 5.7
At3g01100.1 68416.m00015 early-responsive to dehydration protein... 28 5.7
At1g01320.1 68414.m00048 tetratricopeptide repeat (TPR)-containi... 28 5.7
At5g13540.2 68418.m01564 expressed protein HERC2 - Homo sapiens,... 27 7.5
At5g13540.1 68418.m01563 expressed protein HERC2 - Homo sapiens,... 27 7.5
At2g04865.1 68415.m00502 expressed protein ; expression supporte... 27 7.5
At1g28400.1 68414.m03492 expressed protein similar to E6 (GI:100... 27 7.5
At4g17565.1 68417.m02626 F-box family protein contains F-box dom... 27 10.0
At3g50050.1 68416.m05472 aspartyl protease family protein contai... 27 10.0
At2g27790.1 68415.m03369 expressed protein 27 10.0
>At1g14770.2 68414.m01766 expressed protein
Length = 429
Score = 35.9 bits (79), Expect = 0.022
Identities = 19/49 (38%), Positives = 26/49 (53%)
Frame = -1
Query: 408 PRDEANFSVGKNEKIDSDEQMIGIAANLQTTEKDEVKTGDKESDKIETS 262
P DE + +G NEK DEQ + I L EKD+V D E + + T+
Sbjct: 203 PTDEQHVHIG-NEKESIDEQQVHIGLELNRNEKDKVIAIDDEDEPMHTN 250
>At1g14770.1 68414.m01765 expressed protein
Length = 429
Score = 35.9 bits (79), Expect = 0.022
Identities = 19/49 (38%), Positives = 26/49 (53%)
Frame = -1
Query: 408 PRDEANFSVGKNEKIDSDEQMIGIAANLQTTEKDEVKTGDKESDKIETS 262
P DE + +G NEK DEQ + I L EKD+V D E + + T+
Sbjct: 203 PTDEQHVHIG-NEKESIDEQQVHIGLELNRNEKDKVIAIDDEDEPMHTN 250
>At2g22795.1 68415.m02704 expressed protein
Length = 734
Score = 31.1 bits (67), Expect = 0.61
Identities = 27/101 (26%), Positives = 44/101 (43%), Gaps = 4/101 (3%)
Frame = -1
Query: 477 EPFSFXETQSLEYTTSTTQDIISPRDEANFSVGKNEKIDSDEQMIGIAANLQTTEKDEV- 301
E S E++ E T ++ S + + EK +S Q + + EK E
Sbjct: 421 EVSSQEESKGKESETKDKEESSSQEESKDRETETKEKEESSSQEETMDKETEAKEKVESS 480
Query: 300 ---KTGDKESDKIETSGIERM*NVKTHTKLKQTIKSLIKSK 187
K DKE++KIE+S +E + TK K+ S K++
Sbjct: 481 SQEKNEDKETEKIESSFLEETKEKEDETKEKEESSSQEKTE 521
Score = 29.5 bits (63), Expect = 1.9
Identities = 17/41 (41%), Positives = 26/41 (63%), Gaps = 1/41 (2%)
Frame = -3
Query: 244 KREDTYEVKTDDKKSNKIETSGIIENVERE-ETIEPELSDT 125
K E + + K +DK++ KIE+S + E E+E ET E E S +
Sbjct: 476 KVESSSQEKNEDKETEKIESSFLEETKEKEDETKEKEESSS 516
>At3g48860.2 68416.m05337 expressed protein
Length = 577
Score = 30.3 bits (65), Expect = 1.1
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = -1
Query: 483 EXEPFSFXETQSLEYTTSTTQDIISPRDEANFSVGKNEKIDSDEQMIGIAANLQTTEKDE 304
E + S E SLE + ++ + EA +V K +K DE+++ + + L+ KDE
Sbjct: 253 EKQVASLGEGVSLEAKLLSRKEAALRQREAALNVAKQKKSGKDEEIVSLRSELENL-KDE 311
Query: 303 VKTGDKESDKIET 265
T + + E+
Sbjct: 312 ATTAAERLQEAES 324
>At3g48860.1 68416.m05336 expressed protein
Length = 494
Score = 30.3 bits (65), Expect = 1.1
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = -1
Query: 483 EXEPFSFXETQSLEYTTSTTQDIISPRDEANFSVGKNEKIDSDEQMIGIAANLQTTEKDE 304
E + S E SLE + ++ + EA +V K +K DE+++ + + L+ KDE
Sbjct: 253 EKQVASLGEGVSLEAKLLSRKEAALRQREAALNVAKQKKSGKDEEIVSLRSELENL-KDE 311
Query: 303 VKTGDKESDKIET 265
T + + E+
Sbjct: 312 ATTAAERLQEAES 324
>At1g23280.1 68414.m02912 MAK16 protein-related contains similarity
to MAK16 protein SP:P10962 from [Saccharomyces
cerevisiae]
Length = 303
Score = 30.3 bits (65), Expect = 1.1
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -3
Query: 226 EVKTDDKKSNKIETSGIIENVEREETIEPELSDTLSD 116
E++T+D+ + E G+IE VE ++ +E E + + D
Sbjct: 193 EIETNDEVEKEEEEEGVIEYVEGDDELEAEEEEDMED 229
>At1g75310.1 68414.m08748 DNAJ heat shock N-terminal domain-containing
protein low similarity to SP|Q27974 Auxilin {Bos taurus};
contains Pfam profile PF00226: DnaJ domain
Length = 1448
Score = 29.5 bits (63), Expect = 1.9
Identities = 18/62 (29%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
Frame = -1
Query: 432 STTQDIISPRDEANFSVGKNEKIDSDEQMIGIAANLQTTEKDEVKTGDKESDKIETS-GI 256
S T++ I D + G + D D + A+ ++ DE++ +ESD+++TS G+
Sbjct: 976 SNTEEHIEEIDSDSIQSGWSVVEDDDRSLQDGGASQAESKHDELEETKEESDEMKTSLGV 1035
Query: 255 ER 250
ER
Sbjct: 1036 ER 1037
>At3g12810.1 68416.m01598 SNF2 domain-containing protein / helicase
domain-containing protein similar to transcriptional
activator SRCAP [Homo sapiens] GI:5106572; contains Pfam
profiles PF00271: Helicase conserved C-terminal domain,
PF00176: SNF2 family N-terminal domain
Length = 2055
Score = 29.1 bits (62), Expect = 2.5
Identities = 18/67 (26%), Positives = 31/67 (46%), Gaps = 5/67 (7%)
Frame = -2
Query: 569 SHQLERNKLTVTKIDDAHVSKAWPILCWTNXSRFRSRKHNL---SNIQHQRLKI--LFHL 405
S +R K +D+AH+ K W W F S++ L + +Q+ +++ L H
Sbjct: 650 SKMFKRKKWKYLILDEAHLIKNWKSQRWQTLLNFNSKRRILLTGTPLQNDLMELWSLMHF 709
Query: 404 EMKPIFQ 384
M +FQ
Sbjct: 710 LMPHVFQ 716
>At5g20680.1 68418.m02456 expressed protein predicted proteins,
Arabidopsis thaliana
Length = 551
Score = 28.7 bits (61), Expect = 3.3
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -3
Query: 163 EREETIEPELSDTLSDAVPINVVDPITNNHINLKPDNFWINKY 35
E T+ S TL D P+N+ DP T + ++L ++ +Y
Sbjct: 337 ETNTTVLYHWSSTLCDIEPLNITDPATEHAMHLDRPPAFLRQY 379
>At3g53670.1 68416.m05927 expressed protein
Length = 203
Score = 28.3 bits (60), Expect = 4.3
Identities = 12/44 (27%), Positives = 26/44 (59%)
Frame = -3
Query: 223 VKTDDKKSNKIETSGIIENVEREETIEPELSDTLSDAVPINVVD 92
V +DD + + + SG I EE+ + +S+ + +++P+N +D
Sbjct: 34 VGSDDAQESDGDDSGYIHQTVIEESKDKAISEPIPESLPLNSLD 77
>At3g10070.1 68416.m01207 transcription initiation factor IID
(TFIID) subunit A family protein similar to hypothetical
protein GB:CAB10099 [Schizosaccharomyces pombe];
contains Pfam profile PF03847: Transcription initiation
factor TFIID subunit A
Length = 539
Score = 27.9 bits (59), Expect = 5.7
Identities = 13/45 (28%), Positives = 26/45 (57%)
Frame = -3
Query: 217 TDDKKSNKIETSGIIENVEREETIEPELSDTLSDAVPINVVDPIT 83
+DD+ K +++ ++ E ++PE+ D LSD + + V+ IT
Sbjct: 395 SDDRILGKRSIHELLQQIDPSEKLDPEVEDILSD-IAEDFVESIT 438
>At3g01100.1 68416.m00015 early-responsive to dehydration
protein-related / ERD protein-related low similarity to
ERD4 protein (early-responsive to dehydration stress)
[Arabidopsis thaliana] GI:15375406; contains Pfam
profile PF02714: Domain of unknown function DUF221
Length = 703
Score = 27.9 bits (59), Expect = 5.7
Identities = 23/81 (28%), Positives = 35/81 (43%), Gaps = 8/81 (9%)
Frame = +2
Query: 125 CIGQFRF-NSLFTFYIL-YNTGS------FDFIRLFIVCFNFVCVFTFHILSIPEVSILS 280
C+G + N L Y Y TG + +V + + V F + +P S L+
Sbjct: 574 CLGYIIYRNQLLNVYAAKYETGGKFWPIVHSYTIFSLVLMHIIAVGLFGLKELPVASSLT 633
Query: 281 DSLSPVLTSSFSVVCKLAAMP 343
L PVLT FS+ C+ +P
Sbjct: 634 IPL-PVLTVLFSIYCQRRFLP 653
>At1g01320.1 68414.m00048 tetratricopeptide repeat (TPR)-containing
protein low similarity to SP|P46825 Kinesin light chain
(KLC) {Loligo pealeii}; contains Pfam profile PF00515:
TPR Domain
Length = 1797
Score = 27.9 bits (59), Expect = 5.7
Identities = 21/85 (24%), Positives = 44/85 (51%), Gaps = 8/85 (9%)
Frame = -1
Query: 432 STTQDIISPRDEANFSVGKNEKIDSDEQM---IGIA--ANLQ---TTEKDEVKTGDKESD 277
+++++ +S E + + + E+ID D M +G +LQ TEKD+ +TG+K +
Sbjct: 522 TSSREFVSKMLEESIAKLEGEEIDRDSIMRWELGACWIQHLQDQKNTEKDKKQTGEKSKN 581
Query: 276 KIETSGIERM*NVKTHTKLKQTIKS 202
+++ G+ + +K K + S
Sbjct: 582 ELKVEGLGKPLKSLNSSKKKTDVSS 606
>At5g13540.2 68418.m01564 expressed protein HERC2 - Homo sapiens,
EMBL:AF071172; isoform contains non-consensus GG
acceptor splice site at intron 6
Length = 788
Score = 27.5 bits (58), Expect = 7.5
Identities = 21/71 (29%), Positives = 30/71 (42%)
Frame = -1
Query: 495 SVLDEXEPFSFXETQSLEYTTSTTQDIISPRDEANFSVGKNEKIDSDEQMIGIAANLQTT 316
SV D + F Q + T S TQ + +E ++GK +ID D + QT
Sbjct: 332 SVTDVEKVTPFHVGQEIHMTPSITQPRLGWSNETPATIGKVMRIDMDGTLSAQVTGRQTL 391
Query: 315 EKDEVKTGDKE 283
+ V GD E
Sbjct: 392 WR--VSPGDAE 400
>At5g13540.1 68418.m01563 expressed protein HERC2 - Homo sapiens,
EMBL:AF071172; isoform contains non-consensus GG
acceptor splice site at intron 6
Length = 558
Score = 27.5 bits (58), Expect = 7.5
Identities = 21/71 (29%), Positives = 30/71 (42%)
Frame = -1
Query: 495 SVLDEXEPFSFXETQSLEYTTSTTQDIISPRDEANFSVGKNEKIDSDEQMIGIAANLQTT 316
SV D + F Q + T S TQ + +E ++GK +ID D + QT
Sbjct: 336 SVTDVEKVTPFHVGQEIHMTPSITQPRLGWSNETPATIGKVMRIDMDGTLSAQVTGRQTL 395
Query: 315 EKDEVKTGDKE 283
+ V GD E
Sbjct: 396 WR--VSPGDAE 404
>At2g04865.1 68415.m00502 expressed protein ; expression supported
by MPSS
Length = 667
Score = 27.5 bits (58), Expect = 7.5
Identities = 16/47 (34%), Positives = 28/47 (59%), Gaps = 1/47 (2%)
Frame = -3
Query: 250 NVKREDTY-EVKTDDKKSNKIETSGIIENVEREETIEPELSDTLSDA 113
+VK+ED +V+ DD + SG EN REE E E+ ++++++
Sbjct: 609 DVKKEDKESKVEDDDAAKGFSDVSGE-ENANREEEDETEMGESVAES 654
>At1g28400.1 68414.m03492 expressed protein similar to E6
(GI:1000090) [Gossypium barbadense]
Length = 335
Score = 27.5 bits (58), Expect = 7.5
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = -3
Query: 274 NRNFWY*ENVKREDTYEVKTDDKKSNKIETSGIIENVERE 155
N N+ Y ENVK E+++ +D K N ++ +ERE
Sbjct: 189 NYNYKYDENVK-EESFPENNEDNKKNVYNSNAYGTELERE 227
>At4g17565.1 68417.m02626 F-box family protein contains F-box domain
Pfam:PF00646
Length = 378
Score = 27.1 bits (57), Expect = 10.0
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = -2
Query: 551 NKLTVTKIDDAHVSKAWPILCWTNXSRFRSRKHNLSNIQHQRLKI 417
N LT+ +ID H + P +C+ N +RS ++NI L +
Sbjct: 127 NPLTLERIDLPHSTSINPNMCYPNFKYYRSACLWINNITKDYLVV 171
>At3g50050.1 68416.m05472 aspartyl protease family protein contains
Pfam PF00026: Eukaryotic aspartyl protease
Length = 632
Score = 27.1 bits (57), Expect = 10.0
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 4/58 (6%)
Frame = -3
Query: 211 DKKSNKIETSGII----ENVEREETIEPELSDTLSDAVPINVVDPITNNHINLKPDNF 50
D KS+++ S + E++ R + PE S S+ N+V TN+ I L P+ F
Sbjct: 507 DVKSSQVSLSNLTSKGNESLVRMVVLPPEPSTWFSNVTATNIVSRFTNHQIKL-PEIF 563
>At2g27790.1 68415.m03369 expressed protein
Length = 240
Score = 27.1 bits (57), Expect = 10.0
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = -3
Query: 223 VKTDDKKSNKIETSGIIENVEREETIEPELSDTLSDAVPINVVDP 89
VKT +K NKIE SG + ++ +PE S P + + P
Sbjct: 183 VKTVREKLNKIEESGNQKRLQPSSQTQPEESGNQKRLQPSSQIQP 227
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,257,682
Number of Sequences: 28952
Number of extensions: 202776
Number of successful extensions: 762
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 721
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 759
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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