BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0108.Seq (618 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 29 0.41 SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 3.8 SPAPB1A10.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 3.8 SPAC27E2.05 |cdc1|mis1|DNA polymerase delta small subunit Cdc1|S... 25 6.6 SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 25 6.6 SPBP4H10.20 |nhm1|DcpS|m7G|Schizosaccharomyces pombe|chr 2|||Manual 25 6.6 SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pom... 25 6.6 SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 25 8.8 SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosa... 25 8.8 >SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 406 Score = 29.5 bits (63), Expect = 0.41 Identities = 15/69 (21%), Positives = 30/69 (43%) Frame = +3 Query: 171 IAEKLQSDRKRNGYQREPKRY*QMCLLGPITPAIQTARTKSISEAKKAFLNATVSPNAPP 350 + EK + + K+N Q +P+ Y ++ PA+ + + ++K + SP P Sbjct: 99 VKEKDRLESKKNERQGKPRSYNELLRQASSAPAVNETSSSGLLQSKDKRSQSPHSPKKPV 158 Query: 351 TKPDKRIEP 377 R +P Sbjct: 159 KNSSSRDQP 167 >SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 751 Score = 26.2 bits (55), Expect = 3.8 Identities = 22/79 (27%), Positives = 38/79 (48%), Gaps = 4/79 (5%) Frame = +3 Query: 255 PITPAIQTARTKSISEAKKAFLNATVSPNAPPTKPDKRIEPTVHYAQLDIITK--SEDTR 428 P + ++ ++K+++ N T +P + K KRI+PT A L+ +T+ +R Sbjct: 296 PTKKSNRSEQSKTVANTNVGSKNGT-TPRSFAQKSSKRIKPTEGSANLNTVTELTGIGSR 354 Query: 429 STGGGHDLKTS--SDVKSK 479 G +K S S KSK Sbjct: 355 IAMNGSSVKASNISTEKSK 373 Score = 25.8 bits (54), Expect = 5.0 Identities = 29/99 (29%), Positives = 45/99 (45%), Gaps = 4/99 (4%) Frame = +3 Query: 285 TKSISEAKKAFLNATVSPNAP--PTKPDKRIEPTVHYAQLDIITKSEDTRSTGGGHDLKT 458 TK +S+ +KAFLN +PNA ++ D+ E T+ A SE T+ + KT Sbjct: 255 TKLVSK-EKAFLNTVTTPNADLIISEEDELTEMTLRTA-----NGSEPTKKSNRSEQSKT 308 Query: 459 --SSDVKSKETPNEATHAGVMLKRKIDPNQRKVSFKIKT 569 +++V SK + A KR I P + + T Sbjct: 309 VANTNVGSKNGTTPRSFAQKSSKR-IKPTEGSANLNTVT 346 >SPAPB1A10.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 26.2 bits (55), Expect = 3.8 Identities = 25/83 (30%), Positives = 34/83 (40%), Gaps = 1/83 (1%) Frame = +3 Query: 261 TPAIQTARTKSISEAKKAFLNATVSPNAPPTKPDKRIEPTVHYAQLDIITKSEDTRSTGG 440 T + + A TKS EA + L T SP+ P K E + K ED S+ Sbjct: 111 TGSTKAADTKSSVEATISPLKDTKSPSNASVFPIKPTESKTSTTKDSETAKEEDDASSST 170 Query: 441 GHDLK-TSSDVKSKETPNEATHA 506 ++ T+S S T AT A Sbjct: 171 TKAVEATTSKASSAHTDTLATSA 193 >SPAC27E2.05 |cdc1|mis1|DNA polymerase delta small subunit Cdc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 462 Score = 25.4 bits (53), Expect = 6.6 Identities = 13/37 (35%), Positives = 18/37 (48%) Frame = -2 Query: 599 DSRXPVECSGSFNFKADFSLIRIYFSL*HDSSVGRFV 489 D ++CSGSF + A + L H+ GRFV Sbjct: 122 DDYGRIDCSGSFLYDAGVVTGVVLAVLGHEDEQGRFV 158 >SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 386 Score = 25.4 bits (53), Expect = 6.6 Identities = 13/35 (37%), Positives = 21/35 (60%) Frame = -2 Query: 290 FRSSCLYRWRYGTE*THLLVSFWFSLIAISFSVTL 186 +R +CL R+ +G + LLV F FS++ I + L Sbjct: 149 WRHTCL-RYPFGMQQMELLVDFSFSILLIFLGMNL 182 >SPBP4H10.20 |nhm1|DcpS|m7G|Schizosaccharomyces pombe|chr 2|||Manual Length = 304 Score = 25.4 bits (53), Expect = 6.6 Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 2/51 (3%) Frame = +1 Query: 79 TSRLLKLMEAVHTKELIIISDDAKQHGTVKALLRNFRVTE--KEMAIKENQ 225 T L LM VH ++ I D +H + +RN +TE K+ ++ +NQ Sbjct: 179 TMSALNLMAIVHATDIASIRDLKYKHIPLLENIRNKVLTEVPKQFSVDKNQ 229 >SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 569 Score = 25.4 bits (53), Expect = 6.6 Identities = 21/70 (30%), Positives = 33/70 (47%) Frame = +3 Query: 276 TARTKSISEAKKAFLNATVSPNAPPTKPDKRIEPTVHYAQLDIITKSEDTRSTGGGHDLK 455 TA +EAK A A + + + +K + + A L + +DT+ T K Sbjct: 396 TAEISEKTEAKNADSEANLEEKSDVGE-EKESKDENNKASLHKDVEEKDTKITNEDTG-K 453 Query: 456 TSSDVKSKET 485 T +DVK+KET Sbjct: 454 TETDVKAKET 463 >SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 25.0 bits (52), Expect = 8.8 Identities = 18/50 (36%), Positives = 30/50 (60%), Gaps = 1/50 (2%) Frame = +1 Query: 85 RLLKLMEAVHTKELIIISDDAKQHG-TVKALLRNFRVTEKEMAIKENQND 231 ++L L+E +E IS ++ TVK L R R++E EMA+KE +++ Sbjct: 323 KILDLLEMKQQEENDRISHIEYENDLTVKKLKR--RISELEMAVKEYESE 370 >SPAC30D11.09 |cwf19||complexed with Cdc5 protein Cwf19 |Schizosaccharomyces pombe|chr 1|||Manual Length = 639 Score = 25.0 bits (52), Expect = 8.8 Identities = 12/33 (36%), Positives = 19/33 (57%) Frame = -1 Query: 510 LQRGSLRLAFLCS*HHLMFLSHVLRQCFSCLRF 412 ++RG + LA + S L ++HVL C CL + Sbjct: 386 MKRGDVSLAQISSGGDLKKINHVLDTCPLCLNY 418 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,234,868 Number of Sequences: 5004 Number of extensions: 41673 Number of successful extensions: 138 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 128 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 138 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 271646730 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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