Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= msgV0105.Seq
         (648 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q8T7L8 Cluster: Seroin 1; n=1; Bombyx mori|Rep: Seroin ...   140   3e-32
UniRef50_Q8T7L7 Cluster: Seroin 2; n=1; Bombyx mori|Rep: Seroin ...    60   6e-08
UniRef50_O76192 Cluster: Seroin precursor; n=1; Galleria mellone...    36   0.64 
UniRef50_Q0Q006 Cluster: Serpin-like protein; n=1; Antheraea myl...    34   2.6  

>UniRef50_Q8T7L8 Cluster: Seroin 1; n=1; Bombyx mori|Rep: Seroin 1 -
           Bombyx mori (Silk moth)
          Length = 108

 Score =  140 bits (339), Expect = 3e-32
 Identities = 65/66 (98%), Positives = 65/66 (98%)
 Frame = +3

Query: 33  MAFTKFLFVITLITIASAGFVWEDDDDLFPGFSDTFKMPEIPEIKSLEFDDIKTHVAGDN 212
           MAFTKFLFVITLITIASAGFVWEDDDDLFPGFSDTFKM EIPEIKSLEFDDIKTHVAGDN
Sbjct: 1   MAFTKFLFVITLITIASAGFVWEDDDDLFPGFSDTFKMREIPEIKSLEFDDIKTHVAGDN 60

Query: 213 EQYTGE 230
           EQYTGE
Sbjct: 61  EQYTGE 66



 Score = 71.7 bits (168), Expect = 1e-11
 Identities = 33/34 (97%), Positives = 34/34 (100%)
 Frame = +2

Query: 254 TTVNGKTVSSGGVSELTNDGKAVEEKVMEYKDGD 355
           +TVNGKTVSSGGVSELTNDGKAVEEKVMEYKDGD
Sbjct: 75  STVNGKTVSSGGVSELTNDGKAVEEKVMEYKDGD 108


>UniRef50_Q8T7L7 Cluster: Seroin 2; n=1; Bombyx mori|Rep: Seroin 2 -
           Bombyx mori (Silk moth)
          Length = 112

 Score = 59.7 bits (138), Expect = 6e-08
 Identities = 30/45 (66%), Positives = 37/45 (82%), Gaps = 1/45 (2%)
 Frame = +3

Query: 33  MAFTKFLFVITLITIASAGFVWEDDDDLFPGF-SDTFKMPEIPEI 164
           MAFTKFLF+++LITIASAGFVW+DD+  FPGF SD +   +IP I
Sbjct: 1   MAFTKFLFMLSLITIASAGFVWQDDN--FPGFPSDMWPSIQIPTI 43


>UniRef50_O76192 Cluster: Seroin precursor; n=1; Galleria
           mellonella|Rep: Seroin precursor - Galleria mellonella
           (Wax moth)
          Length = 167

 Score = 36.3 bits (80), Expect = 0.64
 Identities = 16/41 (39%), Positives = 26/41 (63%), Gaps = 1/41 (2%)
 Frame = +3

Query: 42  TKFLFVITLITIASAGFVWEDDD-DLFPGFSDTFKMPEIPE 161
           TK L  ++ + ++SAGFVW DDD + FP     + +P +P+
Sbjct: 3   TKILIFLSFVALSSAGFVWVDDDNNSFPKLRQLY-VPPLPQ 42


>UniRef50_Q0Q006 Cluster: Serpin-like protein; n=1; Antheraea
           mylitta|Rep: Serpin-like protein - Antheraea mylitta
           (Tasar silkworm)
          Length = 158

 Score = 34.3 bits (75), Expect = 2.6
 Identities = 12/30 (40%), Positives = 20/30 (66%)
 Frame = +3

Query: 33  MAFTKFLFVITLITIASAGFVWEDDDDLFP 122
           MA TK    ++L+ +++A  +W +DDD FP
Sbjct: 1   MALTKIFLALSLVALSNAVLMWPNDDDRFP 30


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 537,739,414
Number of Sequences: 1657284
Number of extensions: 9620550
Number of successful extensions: 23625
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 22958
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23611
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 48955894634
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)