BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= msgV0104.Seq (469 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 136 1e-33 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 136 1e-33 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 74 9e-15 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 72 4e-14 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 41 1e-04 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 31 0.066 SPBC1734.13 |atp3||F1-ATPase gamma subunit |Schizosaccharomyces ... 27 1.1 SPBC887.04c |lub1||WD repeat protein Lub1|Schizosaccharomyces po... 27 1.1 SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 27 1.1 SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual 27 1.9 SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 26 2.5 SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 4.4 SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|c... 25 4.4 SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyce... 25 7.6 SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|... 25 7.6 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 7.6 SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 25 7.6 SPCC550.07 |||acetamidase |Schizosaccharomyces pombe|chr 3|||Manual 25 7.6 SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 25 7.6 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 136 bits (330), Expect = 1e-33 Identities = 64/87 (73%), Positives = 69/87 (79%) Frame = +2 Query: 2 EGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRE 181 EGLKRL+KSDP V C ESGEHIVAGAGELHLEICLKDL+EDHA IP+K S PVV YRE Sbjct: 506 EGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRE 565 Query: 182 TVXEESDQLCLSKSPNKHNRLFMKVSP 262 +V E S LSKSPNKHNR+FM P Sbjct: 566 SVSEPSSMTALSKSPNKHNRIFMTAEP 592 Score = 51.2 bits (117), Expect = 8e-08 Identities = 22/48 (45%), Positives = 30/48 (62%) Frame = +1 Query: 256 QPMPDGLPEDIDEGRVNPRDDFKTRARYLTEXYEYDVTEARXFGALAP 399 +PM + L I+ G VNPRDDFK RAR + + + +DVT+AR P Sbjct: 591 EPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGP 638 Score = 44.8 bits (101), Expect = 7e-06 Identities = 17/26 (65%), Positives = 21/26 (80%) Frame = +3 Query: 381 IWCFGPEGTGPNILVDCSKGVQYLNE 458 IWCFGP+ TG N++VD +K V YLNE Sbjct: 633 IWCFGPDTTGANVVVDQTKAVAYLNE 658 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 136 bits (330), Expect = 1e-33 Identities = 64/87 (73%), Positives = 69/87 (79%) Frame = +2 Query: 2 EGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRE 181 EGLKRL+KSDP V C ESGEHIVAGAGELHLEICLKDL+EDHA IP+K S PVV YRE Sbjct: 506 EGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRE 565 Query: 182 TVXEESDQLCLSKSPNKHNRLFMKVSP 262 +V E S LSKSPNKHNR+FM P Sbjct: 566 SVSEPSSMTALSKSPNKHNRIFMTAEP 592 Score = 51.2 bits (117), Expect = 8e-08 Identities = 22/48 (45%), Positives = 30/48 (62%) Frame = +1 Query: 256 QPMPDGLPEDIDEGRVNPRDDFKTRARYLTEXYEYDVTEARXFGALAP 399 +PM + L I+ G VNPRDDFK RAR + + + +DVT+AR P Sbjct: 591 EPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWCFGP 638 Score = 44.8 bits (101), Expect = 7e-06 Identities = 17/26 (65%), Positives = 21/26 (80%) Frame = +3 Query: 381 IWCFGPEGTGPNILVDCSKGVQYLNE 458 IWCFGP+ TG N++VD +K V YLNE Sbjct: 633 IWCFGPDTTGANVVVDQTKAVAYLNE 658 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 74.1 bits (174), Expect = 9e-15 Identities = 37/72 (51%), Positives = 46/72 (63%) Frame = +2 Query: 5 GLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRET 184 GL L ++DP VQ EE+GEH++ AGE+HLE CLKDL E A I I+ S P+V YRET Sbjct: 582 GLDMLNQADPCVQIAVEENGEHVIMCAGEIHLERCLKDLRERFAKIEIQASQPLVPYRET 641 Query: 185 VXEESDQLCLSK 220 D L +K Sbjct: 642 TIATPDLLAKNK 653 Score = 25.8 bits (54), Expect = 3.3 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = +3 Query: 381 IWCFGPEGTGPNILVDCSK 437 I FGP+ GPNIL D +K Sbjct: 751 IIAFGPKRVGPNILFDKTK 769 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 72.1 bits (169), Expect = 4e-14 Identities = 36/87 (41%), Positives = 52/87 (59%) Frame = +2 Query: 2 EGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRE 181 +GL++ KS P+ EESGEH + G GE++++ L DL ++ I I+ SDPV + E Sbjct: 618 DGLRKTNKSYPLSITKVEESGEHTIFGTGEMYMDCLLYDLRTLYSEIEIRVSDPVARFCE 677 Query: 182 TVXEESDQLCLSKSPNKHNRLFMKVSP 262 T + S C S +PNK NR+ M V P Sbjct: 678 TAVDTSSIKCFSDTPNKKNRITMVVEP 704 Score = 31.9 bits (69), Expect = 0.050 Identities = 13/48 (27%), Positives = 25/48 (52%) Frame = +1 Query: 256 QPMPDGLPEDIDEGRVNPRDDFKTRARYLTEXYEYDVTEARXFGALAP 399 +P+ G+ DI+ G+VN K + + + Y++D+ +R A P Sbjct: 703 EPLEKGISNDIENGKVNINWPQKRISEFFQKNYDWDLLASRSIWAFGP 750 Score = 27.1 bits (57), Expect = 1.4 Identities = 11/19 (57%), Positives = 12/19 (63%) Frame = +3 Query: 372 SPXIWCFGPEGTGPNILVD 428 S IW FGP+ G NIL D Sbjct: 742 SRSIWAFGPDDRGTNILRD 760 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 40.7 bits (91), Expect = 1e-04 Identities = 20/66 (30%), Positives = 39/66 (59%), Gaps = 1/66 (1%) Frame = +2 Query: 2 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYR 178 + L R + DP + ++ ES E I++G GELHLE+ ++ + ++ + + P V +R Sbjct: 492 KALNRFQREDPTFRVQLDNESKETIISGMGELHLEVYVERMRREYK-VDCETGKPRVAFR 550 Query: 179 ETVXEE 196 ET+ ++ Sbjct: 551 ETLSKK 556 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 31.5 bits (68), Expect = 0.066 Identities = 20/80 (25%), Positives = 36/80 (45%), Gaps = 1/80 (1%) Frame = +2 Query: 2 EGLKRLAKSDPMVQCINE-ESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYR 178 E L + + DP + + E+G+ ++ G G +HL++ + L + V YR Sbjct: 462 EALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQVSYERLVSEFGA-RASLGKVQVGYR 520 Query: 179 ETVXEESDQLCLSKSPNKHN 238 ET+ + S + NK N Sbjct: 521 ETLIDVSFNSVTLSTENKEN 540 >SPBC1734.13 |atp3||F1-ATPase gamma subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 301 Score = 27.5 bits (58), Expect = 1.1 Identities = 11/38 (28%), Positives = 22/38 (57%) Frame = -2 Query: 333 SASLEVIAGIHATLINVLWQTIRHGLTFMNRRLCLLGD 220 S+ + GIH+++ ++ + + TF N LC+LG+ Sbjct: 102 SSDKGLCGGIHSSISRLIRRELHDPKTFENTSLCILGE 139 >SPBC887.04c |lub1||WD repeat protein Lub1|Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 27.5 bits (58), Expect = 1.1 Identities = 20/81 (24%), Positives = 35/81 (43%) Frame = +2 Query: 140 IPIKKSDPVVXYRETVXEESDQLCLSKSPNKHNRLFMKVSPCLMVCQRTLMRVA*IPAMT 319 +P+ +D VV + E + L K PN ++ K+ + + L A +PAM Sbjct: 417 LPLSYTDEVVKFIEK-NTQGHSLESKKEPNLESQSSNKIKTTIFPVSQLLFSNANVPAMC 475 Query: 320 SRLALGILQKXTNTMLPKPVD 382 RL + +N + K +D Sbjct: 476 QRLR-SLNNTKSNPLPAKSID 495 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 27.5 bits (58), Expect = 1.1 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = +2 Query: 176 RETVXEESDQLCLSKSPNKHNRLFMKVSPCL 268 R T EE + L+KSP+ HNR+ ++P + Sbjct: 466 RLTDDEEREIRALAKSPDIHNRIIASMAPSI 496 >SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual Length = 973 Score = 26.6 bits (56), Expect = 1.9 Identities = 18/56 (32%), Positives = 26/56 (46%) Frame = -2 Query: 195 SSXTVSRYXTTGSDFLIGMQAWSSSRSLRQISRWSSPAPATMCSPDSSLIHCTMGS 28 S T Y TTG+D ++ SS R WS+ P +P ++ I+ T GS Sbjct: 117 SDYTSVYYDTTGTDDVVYEYHPSSERHKSTNDTWSTNLPT---NPTTTAIYSTSGS 169 >SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1253 Score = 26.2 bits (55), Expect = 2.5 Identities = 11/18 (61%), Positives = 14/18 (77%) Frame = +1 Query: 274 LPEDIDEGRVNPRDDFKT 327 LPE IDEG+++ DD KT Sbjct: 194 LPEKIDEGKLSDVDDGKT 211 >SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.4 bits (53), Expect = 4.4 Identities = 10/33 (30%), Positives = 22/33 (66%), Gaps = 2/33 (6%) Frame = -2 Query: 93 SSPAPATMCSPDSSL--IHCTMGSDLARRLRPS 1 S+ +P+T+CSPD+++ ++ G L ++ P+ Sbjct: 403 SNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPN 435 >SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 25.4 bits (53), Expect = 4.4 Identities = 12/24 (50%), Positives = 14/24 (58%) Frame = -1 Query: 394 PKHQIXGLR*HRIRXFL*DTERES 323 PKH++ LR HRI FL R S Sbjct: 241 PKHKLTKLRSHRIATFLKGISRRS 264 >SPCC11E10.03 |mug1||dynactin complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 351 Score = 24.6 bits (51), Expect = 7.6 Identities = 11/39 (28%), Positives = 19/39 (48%) Frame = +2 Query: 35 MVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIK 151 M + IN +S + +E CL L+E H +P++ Sbjct: 1 MKEMINLDSSSEPSIVTKQFSVEECLSKLKEQHCYVPMR 39 >SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 24.6 bits (51), Expect = 7.6 Identities = 11/24 (45%), Positives = 16/24 (66%), Gaps = 2/24 (8%) Frame = +3 Query: 393 GPEGTGPNILVD--CSKGVQYLNE 458 GP G+G ++LVD S+ Q +NE Sbjct: 582 GPRGSGKSVLVDDILSRAAQEINE 605 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 24.6 bits (51), Expect = 7.6 Identities = 12/43 (27%), Positives = 17/43 (39%) Frame = +1 Query: 214 LKVAQQAQPSIHEGQPMPDGLPEDIDEGRVNPRDDFKTRARYL 342 L + P+ +P PDG P + P D K +R L Sbjct: 366 LSIPSTLPPADGVPKPTPDGFPRRFERDPTIPPDSIKVYSRTL 408 >SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1375 Score = 24.6 bits (51), Expect = 7.6 Identities = 10/19 (52%), Positives = 14/19 (73%) Frame = +1 Query: 319 FKTRARYLTEXYEYDVTEA 375 F++R RYLT YE+ V +A Sbjct: 1068 FESRRRYLTNLYEHIVLKA 1086 >SPCC550.07 |||acetamidase |Schizosaccharomyces pombe|chr 3|||Manual Length = 533 Score = 24.6 bits (51), Expect = 7.6 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +2 Query: 11 KRLAKSDPMVQCINEESGEHIVAGAGEL 94 KR A + +V C+NE E +A A EL Sbjct: 83 KRAAIAQQLVNCVNELFYEEALARAAEL 110 >SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 882 Score = 24.6 bits (51), Expect = 7.6 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = -2 Query: 336 PSASLEVIAGIHATLINVLWQTIRHGLT 253 P ASLE + + TLIN ++ +R T Sbjct: 468 PRASLETLCSLCVTLINDTFEPVRMATT 495 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,935,536 Number of Sequences: 5004 Number of extensions: 37403 Number of successful extensions: 130 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 130 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 178394480 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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