Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= msgV0104.Seq
(469 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 127 4e-30
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 85 2e-17
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 84 4e-17
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 84 4e-17
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 68 4e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 44 7e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 44 7e-05
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 39 0.001
At5g27640.1 68418.m03311 eukaryotic translation initiation facto... 29 2.1
At5g25780.1 68418.m03060 eukaryotic translation initiation facto... 29 2.1
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 28 2.7
At1g31480.1 68414.m03854 shoot gravitropism 2 (SGR2) Plant Cell ... 28 2.7
At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger) fa... 28 3.6
At5g23570.1 68418.m02765 XS domain-containing protein / XS zinc ... 28 3.6
At5g17300.1 68418.m02026 myb family transcription factor similar... 27 6.3
At4g08395.1 68417.m01387 hypothetical protein 27 8.4
At3g63180.1 68416.m07097 expressed protein 27 8.4
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 27 8.4
At3g05200.1 68416.m00567 zinc finger (C3HC4-type RING finger) fa... 27 8.4
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 127 bits (306), Expect = 4e-30
Identities = 62/88 (70%), Positives = 70/88 (79%), Gaps = 1/88 (1%)
Frame = +2
Query: 2 EGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDH-ACIPIKKSDPVVXYR 178
EGLKRLAKSDPMV C EESGEHIVAGAGELHLEICLKDL++D I KSDPVV +R
Sbjct: 506 EGLKRLAKSDPMVVCTMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFR 565
Query: 179 ETVXEESDQLCLSKSPNKHNRLFMKVSP 262
ETV + S + +SKSPNKHNRL+M+ P
Sbjct: 566 ETVCDRSTRTVMSKSPNKHNRLYMEARP 593
Score = 52.0 bits (119), Expect = 2e-07
Identities = 23/55 (41%), Positives = 32/55 (58%)
Frame = +1
Query: 250 EGQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEXYEYDVTEARXFGALAPRVPAP 414
E +PM +GL E ID+GR+ PRDD K R++ L E + +D A+ A P P
Sbjct: 590 EARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWAFGPETTGP 644
Score = 49.6 bits (113), Expect = 1e-06
Identities = 20/26 (76%), Positives = 22/26 (84%)
Frame = +3
Query: 381 IWCFGPEGTGPNILVDCSKGVQYLNE 458
IW FGPE TGPN++VD KGVQYLNE
Sbjct: 634 IWAFGPETTGPNMVVDMCKGVQYLNE 659
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 85.0 bits (201), Expect = 2e-17
Identities = 39/87 (44%), Positives = 58/87 (66%)
Frame = +2
Query: 2 EGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRE 181
EGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV + E
Sbjct: 605 EGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVVSFCE 664
Query: 182 TVXEESDQLCLSKSPNKHNRLFMKVSP 262
TV E S C +++PNK N+L M P
Sbjct: 665 TVVESSSMKCFAETPNKKNKLTMIAEP 691
Score = 27.9 bits (59), Expect = 3.6
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +3
Query: 381 IWCFGPEGTGPNILVD 428
IW FGP+ G NIL+D
Sbjct: 732 IWAFGPDKQGTNILLD 747
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 84.2 bits (199), Expect = 4e-17
Identities = 38/87 (43%), Positives = 58/87 (66%)
Frame = +2
Query: 2 EGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRE 181
EGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV + E
Sbjct: 619 EGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCE 678
Query: 182 TVXEESDQLCLSKSPNKHNRLFMKVSP 262
TV E S C +++PNK N++ M P
Sbjct: 679 TVVESSSMKCFAETPNKKNKITMIAEP 705
Score = 31.1 bits (67), Expect = 0.39
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 381 IWCFGPEGTGPNILVD 428
IW FGP+ GPNIL+D
Sbjct: 746 IWAFGPDKQGPNILLD 761
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 84.2 bits (199), Expect = 4e-17
Identities = 38/87 (43%), Positives = 58/87 (66%)
Frame = +2
Query: 2 EGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRE 181
EGL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVV + E
Sbjct: 619 EGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCE 678
Query: 182 TVXEESDQLCLSKSPNKHNRLFMKVSP 262
TV E S C +++PNK N++ M P
Sbjct: 679 TVVESSSMKCFAETPNKKNKITMIAEP 705
Score = 31.1 bits (67), Expect = 0.39
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 381 IWCFGPEGTGPNILVD 428
IW FGP+ GPNIL+D
Sbjct: 746 IWAFGPDKQGPNILLD 761
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 67.7 bits (158), Expect = 4e-12
Identities = 30/69 (43%), Positives = 46/69 (66%)
Frame = +2
Query: 2 EGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRE 181
+GL+ L ++DP V+ GEH++A AGE+HLE C+KDL+E A + ++ S P+V YRE
Sbjct: 556 KGLRLLNRADPFVEITVSARGEHVLAAAGEVHLERCVKDLKERFAKVNLEVSPPLVSYRE 615
Query: 182 TVXEESDQL 208
T+ + L
Sbjct: 616 TIEGDGSNL 624
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 43.6 bits (98), Expect = 7e-05
Identities = 21/68 (30%), Positives = 40/68 (58%), Gaps = 1/68 (1%)
Frame = +2
Query: 2 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYR 178
+ L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V +R
Sbjct: 480 KALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFR 538
Query: 179 ETVXEESD 202
ET+ + ++
Sbjct: 539 ETITQRAE 546
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 43.6 bits (98), Expect = 7e-05
Identities = 21/68 (30%), Positives = 40/68 (58%), Gaps = 1/68 (1%)
Frame = +2
Query: 2 EGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYR 178
+ L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V +R
Sbjct: 480 KALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFR 538
Query: 179 ETVXEESD 202
ET+ + ++
Sbjct: 539 ETITQRAE 546
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 39.1 bits (87), Expect = 0.001
Identities = 22/62 (35%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
Frame = +2
Query: 5 GLKRLAKSDPMVQCI-NEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVXYRE 181
GL +LA+ DP +EE + ++ G GELHLEI + L+ + + P V YRE
Sbjct: 513 GLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRLKREFK-VEANVGAPQVNYRE 571
Query: 182 TV 187
++
Sbjct: 572 SI 573
>At5g27640.1 68418.m03311 eukaryotic translation initiation factor 3
subunit 9 / eIF-3 eta / eIF3b (TIF3B1) nearly identical
to SP|Q9C5Z1 Eukaryotic translation initiation factor 3
subunit 9 (eIF-3 eta) (eIF3 p110) (eIF3b) {Arabidopsis
thaliana}
Length = 712
Score = 28.7 bits (61), Expect = 2.1
Identities = 17/51 (33%), Positives = 23/51 (45%), Gaps = 1/51 (1%)
Frame = -2
Query: 405 YPRGQSTKSTGFGNIVFVXFCK-IPSASLEVIAGIHATLINVLWQTIRHGL 256
Y QS TGFGNI+ V +P E + G+ + N L +GL
Sbjct: 43 YQDDQSEFDTGFGNIIVVDHLPVVPKEKFEKLEGVVKKIYNQLGVIKENGL 93
>At5g25780.1 68418.m03060 eukaryotic translation initiation factor 3
subunit 9, putative / eIF-3 eta, putative / eIF3b,
putative nearly identical to SP|Q9C5Z1 Eukaryotic
translation initiation factor 3 subunit 9 (eIF-3 eta)
(eIF3 p110) (eIF3b) {Arabidopsis thaliana}
Length = 714
Score = 28.7 bits (61), Expect = 2.1
Identities = 17/51 (33%), Positives = 23/51 (45%), Gaps = 1/51 (1%)
Frame = -2
Query: 405 YPRGQSTKSTGFGNIVFVXFCK-IPSASLEVIAGIHATLINVLWQTIRHGL 256
Y QS TGFGNI+ V +P E + G+ + N L +GL
Sbjct: 44 YQDDQSEFDTGFGNIIVVDHLPVVPKEKFEKLEGVVKKIYNQLGVIKENGL 94
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 28.3 bits (60), Expect = 2.7
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +2
Query: 47 INEESGEHIVAGAGELHLEICLKDLEEDH 133
I EE G G G+ ICL++ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At1g31480.1 68414.m03854 shoot gravitropism 2 (SGR2) Plant Cell
2002 Jan;14:33-46 PMID:11826297; similar to
phospholipase [Homo sapiens] GI:4760647; identical to
cDNA PF02862: DDHD domain
Length = 933
Score = 28.3 bits (60), Expect = 2.7
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = -2
Query: 417 CWGRYPRGQSTKSTGFGNIVFVXFCKIPSASLEVIAGIHAT 295
C GR T+S G+ V FCK+P A E+ A + T
Sbjct: 38 CHGRQKYLAQTRSPSDGSDVRWYFCKVPLAENELAASVPRT 78
>At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 161
Score = 27.9 bits (59), Expect = 3.6
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 80 GAGELHLEICLKDLEEDHACIPIKK 154
G E+ ICL++LE+ H I IKK
Sbjct: 111 GFDEIGCSICLEELEDGHEIIRIKK 135
>At5g23570.1 68418.m02765 XS domain-containing protein / XS zinc
finger domain-containing protein-related contains Pfam
profiles PF03468: XS domain, weak hit to PF03470: XS
zinc finger domain
Length = 625
Score = 27.9 bits (59), Expect = 3.6
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -2
Query: 432 SNPPGCWGRYPRGQSTKSTGFGNIV 358
SNPP WG +G+ + +G GN V
Sbjct: 68 SNPPRAWGGQQQGRGSNVSGRGNNV 92
>At5g17300.1 68418.m02026 myb family transcription factor similar to
CCA1 [Arabidopsis thaliana] GI:4090569; contains Pfam
profile PF00249: Myb-like DNA-binding domain
Length = 387
Score = 27.1 bits (57), Expect = 6.3
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = -2
Query: 198 DSSXTVSRYXTTGSDFLIGMQAWSSSRSLRQISRWSSPAPAT 73
D+ S T GS+ L + + S +RSL +S S PA T
Sbjct: 154 DTQSPTSVLSTVGSEALCSLDSSSPNRSLSPVSSASPPAALT 195
>At4g08395.1 68417.m01387 hypothetical protein
Length = 232
Score = 26.6 bits (56), Expect = 8.4
Identities = 13/44 (29%), Positives = 25/44 (56%), Gaps = 3/44 (6%)
Frame = +2
Query: 98 LEICLKDLEEDHACIPIKKSDPVVXYR---ETVXEESDQLCLSK 220
L+ C++ LEEDH+ + ++ +V +T+ +ES L + K
Sbjct: 151 LKTCIETLEEDHSTVMVETCRRMVVVETCIQTLEKESSMLVVEK 194
>At3g63180.1 68416.m07097 expressed protein
Length = 978
Score = 26.6 bits (56), Expect = 8.4
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2
Query: 356 NTMLPKPVDLVLWPRGYRPQHPGGLLQ 436
+++LP PV + WP G PQ L+Q
Sbjct: 382 SSVLPLPVAVASWPSGVPPQGHVALIQ 408
>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 720
Score = 26.6 bits (56), Expect = 8.4
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = -1
Query: 184 GLTVXHDRVRLLDWNASMVLLKILKTDLKMEFSSTSDNV 68
G TV DRV W +++ +++TD +EF N+
Sbjct: 348 GGTVKEDRVSEWLWTLHRIVVDVVRTDSHLEFYEDPGNL 386
>At3g05200.1 68416.m00567 zinc finger (C3HC4-type RING finger)
family protein (ATL6) contains Pfam profile: PF00097:
Zinc finger, C3HC4 type (RING finger)
Length = 398
Score = 26.6 bits (56), Expect = 8.4
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +2
Query: 80 GAGELHLEICLKDLEEDHACIPIKKSDPV 166
G GEL ICL + E+D + K D V
Sbjct: 122 GKGELECAICLNEFEDDETLRLLPKCDHV 150
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,501,997
Number of Sequences: 28952
Number of extensions: 211548
Number of successful extensions: 690
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 689
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 791932800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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